| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 897,968:1 | +A | 13.3% | intergenic (+39/‑21) | potI → / → ybjO | putrescine ABC transporter permease/DUF2593 family inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 897,968 | 1 | . | A | 13.3% | 97.1 / 14.0 | 30 | intergenic (+39/‑21) | potI/ybjO | putrescine ABC transporter permease/DUF2593 family inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (12/14); new base A (2/2); total (14/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.62e-01 | |||||||||||
AACATTGATCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCTGATGA > NC_000913/897821‑898115 | aaCATTGATCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCgcg‑c > 3:249989/1‑149 (MQ=255)aaCATTGATCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCgcg‑c > 3:22316/1‑149 (MQ=255) cATTGATCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CAt < 2:146807/149‑1 (MQ=255) tCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTcgcg < 4:53059/149‑1 (MQ=255) tCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTcgcg > 4:39879/1‑149 (MQ=255) tCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTcgcg < 4:22316/149‑1 (MQ=255) cGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGACATG‑CGCGGCAt < 1:277041/149‑1 (MQ=255) cgGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGttt < 1:176842/149‑1 (MQ=255) gTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGAc < 4:304504/149‑1 (MQ=255) tCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGACATG‑CGCGGCAt < 2:186103/132‑1 (MQ=255) tCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGACATG‑CGCGGCAt > 1:186103/1‑132 (MQ=255) tCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACg > 1:132527/1‑149 (MQ=255) tCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACg > 4:277789/1‑149 (MQ=255) ggTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGAtttt < 2:132527/149‑1 (MQ=255) gATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGaa < 4:400782/149‑1 (MQ=255) cTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACAt < 4:249989/149‑1 (MQ=255) tCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATc > 4:257672/1‑149 (MQ=255) aaCAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGaaaa < 1:421264/134‑1 (MQ=255) aaCAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGaaaa > 2:421264/1‑134 (MQ=255) gATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCt > 2:9030/1‑149 (MQ=255) aGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAg < 1:9030/149‑1 (MQ=255) cTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTggcggc < 3:39879/149‑1 (MQ=255) cTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTggcggc > 3:356223/1‑149 (MQ=255) tGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGGGCTGGTGCAGGTGgcggcg > 3:354500/1‑149 (MQ=255) aGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTaa < 2:438146/88‑1 (MQ=255) aGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTaa > 1:438146/1‑88 (MQ=255) tAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCATGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCatt > 3:299199/1‑149 (MQ=255) aaaTTTGCCAACCTGGCTACATAATGCCCCG‑CATGTCGCGGCATTGTTTTCATGGAAGTCGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCattat < 4:408512/149‑1 (MQ=255) aacaaTGCCGCGACATG‑CGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGAc > 4:394727/4‑149 (MQ=255) tGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGt > 4:164367/1‑101 (MQ=255) tGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGt < 3:164367/101‑1 (MQ=255) tGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCt > 2:170857/1‑149 (MQ=255) cg‑cATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCtgatga < 3:257672/149‑1 (MQ=255) | AACATTGATCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCTGATGA > NC_000913/897821‑898115 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |