New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3527476	41 (0.800)	3 (0.060)	3/240	NT	6.8%	coding (1008/2136 nt)	yrfF	putative RcsCDB‑response attenuator, inner membrane protein
	?	NC_000913	= 3527515	43 (0.890)					coding (1047/2136 nt)	yrfF	putative RcsCDB‑response attenuator, inner membrane protein

TCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCG  >  NC_000913/3527373‑3527658                                                                                                                                               |                                                                                                                                                tcGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGTTGCTGgtgctg                                                                                                                                           <  3:123959‑M2/149‑7 (MQ=255) tcGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGgtgctg                                                                                                                                           <  3:67685‑M2/149‑7 (MQ=255)   ggTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTCTCGCTGCGTGCTGGCTGCTGGTGATGtt                                                                                                                                         <  2:456301/149‑1 (MQ=255)    gTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGttt                                                                                                                                        <  4:84855/149‑1 (MQ=255)      caacaaACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTAt                                                                                                                                      <  3:282155/149‑1 (MQ=255)        acaaACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGc                                                                                                                                    <  2:447128/149‑1 (MQ=255)          aaaCCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTg                                                                                                                                  >  4:406699/1‑149 (MQ=255)           aaCCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGt                                                                                                                                 <  4:438316/149‑1 (MQ=255)            aCCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGtt                                                                                                                                >  4:430491/1‑149 (MQ=255)            aCCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGtt                                                                                                                                <  3:312272/149‑1 (MQ=255)               gatatcgatatcTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTAtt                                                                                                                             >  4:338073/1‑149 (MQ=255)                atatcgatatcTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTc                                                                                                                            <  1:186743/149‑1 (MQ=255)                     gatatcTATCTCGACCGCCATGTAGTGCGGCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGAt                                                                                                                       <  3:430491/149‑1 (MQ=255)                       tatctatcTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATcc                                                                                                                     >  2:475354/1‑149 (MQ=255)                           tatctCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCt                                                                                                                 >  1:66236/1‑149 (MQ=255)                             tctcGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTgg                                                                                                               >  4:334562/1‑149 (MQ=255)                                cGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGTTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGata                                                                                                            <  4:123959/149‑1 (MQ=255)                                  aCCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATg                                                                                                          >  3:174185/1‑149 (MQ=255)                                                cGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCAc                                                                                            <  2:315699/149‑1 (MQ=255)                                                 gTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCAct                                                                                           >  3:100364/1‑149 (MQ=255)                                                     gggACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCActctct                                                                                       <  1:475354/149‑1 (MQ=255)                                                         cGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATg                                                                                   >  1:297892/1‑149 (MQ=255)                                                         cGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATg                                                                                   <  3:406699/149‑1 (MQ=255)                                                               cTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGaa                                                                             >  4:125533/1‑149 (MQ=255)                                                                tttCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGaaa                                                                            <  3:198277/149‑1 (MQ=255)                                                                tttCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGaaa                                                                            >  4:198277/1‑149 (MQ=255)                                                                           gatgaAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGAc                                                                 >  1:70836/1‑149 (MQ=255)                                                                               aaGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGgc                                                                         >  3:190193/1‑137 (MQ=255)                                                                               aaGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGgc                                                                         <  4:190193/137‑1 (MQ=255)                                                                                  tAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGGCCATTGaa                                                          <  3:334562/149‑1 (MQ=255)                                                                                   aaaaaaCTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAg                                                         >  3:376421/1‑149 (MQ=255)                                                                                    aaaaaCTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGc                                                        >  4:291976/1‑149 (MQ=255)                                                                                            tCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCg                                                <  3:338073/149‑1 (MQ=255)                                                                                             ccGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGt                                               <  1:170424/149‑1 (MQ=255)                                                                                                ttACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGgtgctgtaacggaaagttttttacttcatcatgcagcgaaagatatcgtccctgacgcactacatggcggtcgagatagatatcgatatcggtttgttgac                                            <  2:232617‑M2/149‑102 (MQ=255)                                                                                                ttACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTaaa                                            >  2:376180/1‑149 (MQ=255)                                                                                                 tACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCTCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCATCGTaaaa                                           <  2:433989/149‑1 (MQ=255)                                                                                                      cACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGgtgctgtaacggaaagttttttacttcatcatgcagcgaaagatatcgtccctgacgcactacatggcggtcg                                                                        <  3:187209‑M2/115‑74 (MQ=255)                                                                                                      cACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGgtgctgtaacggaaagttttttacttcatcatgcagcgaaagatatcgtccctgacgcactacatggcggtcg                                                                        >  4:187209‑M2/1‑42 (MQ=255)                                                                                                         tGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAAACCATTGAAGCCACCAGCGTAAAACAACTGGc                                   >  4:354757/1‑149 (MQ=255)                                                                                                             tGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCTGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGAc                               <  4:174185/149‑1 (MQ=255)                                                                                                             tGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGaaa                                                                            <  4:11021/104‑1 (MQ=255)                                                                                                             tGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGaaa                                                                            >  3:11021/1‑104 (MQ=255)                                                                                                                gcAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCt                            >  2:330421/1‑149 (MQ=255)                                                                                                                  aGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGTCGCTgg                          <  4:100364/149‑1 (MQ=255)                                                                                                                   gTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGc                         >  2:274231/1‑149 (MQ=255)                                                                                                                     aCGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGt                       <  3:291976/149‑1 (MQ=255)                                                                                                                            tCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTa                >  1:394601/1‑149 (MQ=255)                                                                                                                                      cTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACAt      <  4:67685/149‑1 (MQ=255)                                                                                                                                         gctgctGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGc   >  3:2492/1‑149 (MQ=255)                                                                                                                                          ctgctgGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCg  <  1:330421/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                TCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCG  >  NC_000913/3527373‑3527658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.