Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,272,543 A→C 5.2% K13Q (AAA→CAA)  chaC → cation transport regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,272,5430AC 5.2% 255.6 / 2.6 73K13Q (AAA→CAA) chaCcation transport regulator
Reads supporting (aligned to +/- strand):  ref base A (36/33);  new base C (2/2);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGA  >  NC_000913/1272401‑1272691
                                                                                                                                              |                                                                                                                                                    
cGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACgg                                                                                                                                                >  2:120959/1‑149 (MQ=255)
 gTGGATTGCATATTGTCCCGTTAGGGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGc                                                                                                                                               >  1:139920/1‑149 (MQ=255)
  tGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCa                                                                                                                                              <  3:88104/149‑1 (MQ=255)
        gCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACTAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGTAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGt                                                                                                                                        <  3:435291/149‑1 (MQ=255)
        gCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGt                                                                                                                                        >  3:147818/1‑149 (MQ=255)
        gCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGt                                                                                                                                        <  2:227076/149‑1 (MQ=255)
            atTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCa                                                                                                                                    >  4:329745/1‑149 (MQ=255)
              tGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCAtt                                                                                                                                  <  1:77594/149‑1 (MQ=255)
               gTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTg                                                                                                                                 >  2:335563/1‑149 (MQ=255)
                       aGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCa                                                                                                                         <  4:427376/149‑1 (MQ=255)
                            tttCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCtt                                                                                                                    >  3:372947/1‑149 (MQ=255)
                             ttCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCAt                                                                                                                                             <  3:233082/123‑1 (MQ=255)
                             ttCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCAt                                                                                                                                             >  4:233082/1‑123 (MQ=255)
                               cAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATg                                                                                                                 <  3:212833/149‑1 (MQ=255)
                               cAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATg                                                                                                                 <  2:130693/149‑1 (MQ=255)
                                 aaaTGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGt                                                                                                               >  3:27905/1‑149 (MQ=255)
                                    tGAGCAGTAAAAATGTCCGGAAGACACGAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGACGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTcag                                                                                                            >  2:204535/1‑149 (MQ=255)
                                      aGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTcagca                                                                                                          <  2:147896/149‑1 (MQ=255)
                                        cAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGa                                                                                                        <  3:79279/149‑1 (MQ=255)
                                         aGTAAAAATGTTCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGGGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGaa                                                                                                       >  1:387909/1‑149 (MQ=255)
                                         aGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGaa                                                                                                       <  1:477852/149‑1 (MQ=255)
                                          gTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACAGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGaac                                                                                                      >  2:332894/1‑149 (MQ=255)
                                           tAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGTTTTACAATCGGCAttcatca                                                                                                                                       >  3:185292/1‑110 (MQ=255)
                                           tAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGTTTTACAATCGGCAttcatca                                                                                                                                       <  4:185292/115‑6 (MQ=255)
                                           tAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGaaca                                                                                                     >  2:428845/1‑149 (MQ=255)
                                             aaaaTGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGaacaac                                                                                                   >  2:31745/1‑149 (MQ=255)
                                                             acCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTggc                                                                                   >  2:82042/1‑149 (MQ=255)
                                                               cAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTgaagaa                                                                                                                            <  4:29999/106‑1 (MQ=255)
                                                               cAAAAAGTTGCCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTgaagaa                                                                                                                            >  3:29999/1‑106 (MQ=255)
                                                                aaaaaGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTggcggc                                                                                >  1:252152/1‑149 (MQ=255)
                                                                aaaaaGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTggcggc                                                                                >  4:151229/1‑149 (MQ=255)
                                                                aaaaaGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTggcggc                                                                                >  2:259747/1‑149 (MQ=255)
                                                                 aaaaGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGggc                                                                                               >  2:8775/1‑133 (MQ=255)
                                                                 aaaaGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGggc                                                                                               <  1:8775/133‑1 (MQ=255)
                                                                      ttGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCt                                                                          <  3:151229/149‑1 (MQ=255)
                                                                        gTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTgg                                                                        <  1:18887/149‑1 (MQ=255)
                                                                             aGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTGGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGTGCGGCTTCGCTGGCGGCGACGCTGGCTTGt                                                                   >  1:308591/1‑149 (MQ=255)
                                                                               ggAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCg                                                                 >  2:39932/1‑149 (MQ=255)
                                                                                    tATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCtg                                                            >  3:348715/1‑149 (MQ=255)
                                                                                    tATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCtg                                                            >  3:69895/1‑149 (MQ=255)
                                                                                     aTGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCtga                                                           >  1:377532/1‑149 (MQ=255)
                                                                                     aTGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCtga                                                           <  3:161614/149‑1 (MQ=255)
                                                                                       gCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCtgatg                                                         >  4:70302/1‑149 (MQ=255)
                                                                                         aGTGGCGGAAGTGTAAGGTGATAACGCGTGATGTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCCGAACAACGGGCGACTTCGGTGGCGGCCACGCTGGCTTGTGGAACTGATGAg                                                       >  2:441600/1‑149 (MQ=255)
                                                                                          gTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAgg                                                      <  1:39932/149‑1 (MQ=255)
                                                                                             gCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGAc                                                   <  2:252152/149‑1 (MQ=255)
                                                                                               ggAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCg                                                 >  1:165685/1‑149 (MQ=255)
                                                                                               ggAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCg                                                 >  1:232077/1‑149 (MQ=255)
                                                                                                 aaGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGgt                                               <  4:389671/149‑1 (MQ=255)
                                                                                                   gtgtAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAAGCACTCTTATGGTCAGCAGAACCACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGgtgt                                             <  1:258406/149‑1 (MQ=255)
                                                                                                    tgtAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGtgtg                                            <  3:235464/149‑1 (MQ=255)
                                                                                                           tGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTc                                     <  1:259747/149‑1 (MQ=255)
                                                                                                           tGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGATCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGAGCTGATGAGGGACCGGTGTGGATCTTc                                     >  3:401419/1‑149 (MQ=255)
                                                                                                              tAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACATGATGAGGGACCGGTGTGGATCTTCGGc                                  >  3:277882/1‑149 (MQ=255)
                                                                                                                 cgcgTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTAt                               <  1:332894/149‑1 (MQ=255)
                                                                                                                  gcgTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATg                              >  4:197455/1‑149 (MQ=255)
                                                                                                                      gATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATc                          <  3:329745/149‑1 (MQ=255)
                                                                                                                       aTTTCTGGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCg                         >  2:337972/1‑149 (MQ=255)
                                                                                                                        tttCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGgtgt                                             >  4:254833/1‑128 (MQ=255)
                                                                                                                        tttCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGgtgt                                             <  3:254833/128‑1 (MQ=255)
                                                                                                                        tttCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGt                        <  2:308591/149‑1 (MQ=255)
                                                                                                                         ttCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGtt                       <  3:197455/149‑1 (MQ=255)
                                                                                                                            ttGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGAt                    >  4:366098/1‑149 (MQ=255)
                                                                                                                              gatgaATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGAtgt                  >  3:2240/1‑149 (MQ=255)
                                                                                                                              gatgaATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGAtgt                  <  4:27905/149‑1 (MQ=255)
                                                                                                                               atgaATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGAtgtg                 <  4:348715/149‑1 (MQ=255)
                                                                                                                               atgaATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGAtgtg                 <  4:69895/149‑1 (MQ=255)
                                                                                                                                caaaTGCCGTTTTACAATCGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTgg                >  4:203985/3‑149 (MQ=255)
                                                                                                                                  aaTGCCGTTTTACAATCGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGaa              <  3:203985/149‑1 (MQ=255)
                                                                                                                                  aaTGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGaa              <  2:387909/149‑1 (MQ=255)
                                                                                                                                       cGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCgg         <  3:70302/149‑1 (MQ=255)
                                                                                                                                        gATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGc        <  4:277882/149‑1 (MQ=255)
                                                                                                                                        gATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGc        >  2:347378/1‑149 (MQ=255)
                                                                                                                                              aaaaCGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGa  <  1:337972/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGA  >  NC_000913/1272401‑1272691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: