Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,703,251 A→T 15.1% intergenic (+17/+17) add → / ← ydgJ adenosine deaminase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,703,2510AT15.1% 91.9 / 8.6 33intergenic (+17/+17)add/ydgJadenosine deaminase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (13/15);  new base T (3/2);  total (16/17)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

TACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCGC  >  NC_000913/1703105‑1703399
                                                                                                                                                  |                                                                                                                                                    
taCCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAAt                                                                                                                                                    <  3:337601/149‑1 (MQ=255)
taCCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAAt                                                                                                                                                    >  4:35214/1‑149 (MQ=255)
   cGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCa                                                                                                                                                 <  3:62894/149‑1 (MQ=255)
       gCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCATTTGCAccat                                                                                                                                             <  2:363250/149‑1 (MQ=255)
               aGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCAttttt                                                                                                                                     >  1:74022/1‑149 (MQ=255)
                    ctgGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGc                                                                                                                                >  3:132490/1‑149 (MQ=255)
                     tgGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCa                                                                                                                               >  2:333129/1‑149 (MQ=255)
                         ttATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGc                                                                                                                           <  2:290183/149‑1 (MQ=255)
                                     cAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTcgcgc                                                                                                               <  4:132490/149‑1 (MQ=255)
                                         tCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATg                                                                                                           >  1:102931/1‑149 (MQ=255)
                                             ccAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTAGCGCGATGTTTg                                                                                                       <  3:35214/149‑1 (MQ=255)
                                              cAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTgg                                                                                                      <  3:340165/149‑1 (MQ=255)
                                               aGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGc                                                                                                     >  1:332525/1‑149 (MQ=255)
                                                   acaGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTc                                                                                                                                    <  4:164585/114‑1 (MQ=255)
                                                   acaGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTc                                                                                                                                    >  3:164585/1‑114 (MQ=255)
                                                         tAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATg                                                                                           <  2:332525/149‑1 (MQ=255)
                                                               tCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGGGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGc                                                                                     <  1:333129/149‑1 (MQ=255)
                                                                           tttCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTcc                                                                         >  3:364185/1‑149 (MQ=255)
                                                                              ccTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAAt                                                                                                                                                    >  4:5624/1‑71 (MQ=255)
                                                                              ccTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAAt                                                                                                                                                    <  3:5624/71‑1 (MQ=255)
                                                                                           gAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCt                                                         >  2:396984/1‑149 (MQ=255)
                                                                                                   cgcACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGcc                                                 <  2:74022/149‑1 (MQ=255)
                                                                                                                  ggTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACGAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCa                                  <  3:458999/148‑1 (MQ=255)
                                                                                                                               tAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATc                             >  3:88177/1‑141 (MQ=255)
                                                                                                                               tAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATc                             <  4:88177/141‑1 (MQ=255)
                                                                                                                               tAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTa                     >  1:278622/1‑149 (MQ=255)
                                                                                                                               atgaaaaaTGGATGGTGCATTTGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGccc                                                                                        <  3:197621/78‑1 (MQ=25)
                                                                                                                               atgaaaaaTGGATGGTGCATTTGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGccc                                                                                        >  4:197621/5‑82 (MQ=25)
                                                                                                                                   aaaaTGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGc                 <  1:396984/149‑1 (MQ=255)
                                                                                                                                     aaTGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCAt               >  1:273719/1‑149 (MQ=255)
                                                                                                                                        ggatggTGCATTTGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCAc            >  1:310407/1‑149 (MQ=255)
                                                                                                                                          atgtTGCATTTGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTTCCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGa          >  4:325234/1‑149 (MQ=255)
                                                                                                                                           tggTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGaa         <  2:278622/149‑1 (MQ=255)
                                                                                                                                                  aaTGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCGc  >  2:318170/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCGC  >  NC_000913/1703105‑1703399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: