Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,928,799 A→C 9.4% L14R (CTT→CGT)  ptrB ← protease II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,928,7990AC 9.4% 165.5 / 10.2 53L14R (CTT→CGT) ptrBprotease II
Reads supporting (aligned to +/- strand):  ref base A (21/27);  new base C (3/2);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.27e-01

TTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGG  >  NC_000913/1928652‑1928941
                                                                                                                                                   |                                                                                                                                              
tttAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAg                                                                                                                                               >  1:391427/1‑149 (MQ=255)
   aaGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGt                                                                                                                                            <  1:175111/149‑1 (MQ=255)
                 cAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGaa                                                                                                                              <  3:72426/149‑1 (MQ=255)
                   aGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATg                                                                                                                            <  2:116305/149‑1 (MQ=255)
                       ttgttgTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcggg                                                                                                                        >  3:249968/1‑149 (MQ=255)
                             tgAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCtt                                                                                                                  >  1:372842/1‑149 (MQ=255)
                               aGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCtttt                                                                                                                <  4:13042/149‑1 (MQ=255)
                               aGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCtttt                                                                                                                <  4:349924/149‑1 (MQ=255)
                               aGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCtttt                                                                                                                <  4:57100/149‑1 (MQ=255)
                                 gCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTgg                                                                                                              >  1:103059/1‑149 (MQ=255)
                                      cACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGACGCTTCATGGc                                                                                                                                      <  3:286416/120‑1 (MQ=255)
                                      cACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGACGCTTCATGGc                                                                                                                                      >  4:286416/1‑120 (MQ=255)
                                             tGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTAtt                                                                                                  <  2:267595/149‑1 (MQ=255)
                                               aCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGt                                                                                                >  2:249253/1‑149 (MQ=255)
                                                    aaCTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttcttt                                                                                           <  1:190307/149‑1 (MQ=255)
                                                     aCTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttctttc                                                                                          <  2:465438/149‑1 (MQ=255)
                                                       tATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTg                                                                                        >  1:131335/1‑149 (MQ=255)
                                                        aTTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTgg                                                                                       <  3:278626/149‑1 (MQ=255)
                                                          tttCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGtt                                                                                     >  4:213922/1‑149 (MQ=255)
                                                                  tgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATc                                                                             <  2:409256/149‑1 (MQ=255)
                                                                     aGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTta                                                                          >  4:100851/1‑149 (MQ=255)
                                                                            ccAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGt                                                                   <  1:249253/149‑1 (MQ=255)
                                                                             cAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTCTTGTTCTTTCTGGTTGAAACATCTTATAAGGGTg                                                                  >  3:389040/1‑149 (MQ=255)
                                                                              aGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTgg                                                                 >  2:21351/1‑149 (MQ=255)
                                                                                   ttCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACAt                                                                              >  3:140520/1‑131 (MQ=255)
                                                                                   ttCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACAt                                                                              <  4:140520/131‑1 (MQ=255)
                                                                                   ttCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCaaaa                                                            <  1:162866/149‑1 (MQ=255)
                                                                                      tGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCaaaa                                                            >  4:27299/1‑146 (MQ=255)
                                                                                      tGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCaaaa                                                            <  3:27299/146‑1 (MQ=255)
                                                                                      tGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTc                                                         >  2:464145/1‑149 (MQ=255)
                                                                                          tGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTg                                                                                                               >  1:309752/1‑91 (MQ=255)
                                                                                          tGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTg                                                                                                               <  2:309752/91‑1 (MQ=255)
                                                                                             gaACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCgggg                                                  >  2:436092/1‑149 (MQ=255)
                                                                                                         tCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCtt                                                                           >  3:448201/1‑112 (MQ=255)
                                                                                                         tCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCtt                                                                           <  4:448201/112‑1 (MQ=255)
                                                                                                         tCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGaaa                                      <  2:372842/149‑1 (MQ=255)
                                                                                                             gCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAgg                                  <  3:213922/149‑1 (MQ=255)
                                                                                                             gCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAgg                                  <  3:100851/149‑1 (MQ=255)
                                                                                                                         aaTTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCt                      <  1:216785/149‑1 (MQ=255)
                                                                                                                           ttATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCt                                                                            >  1:111863/1‑93 (MQ=255)
                                                                                                                           ttATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCt                                                                            <  2:111863/93‑1 (MQ=255)
                                                                                                                             aTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCAt                          >  3:151436/1‑141 (MQ=255)
                                                                                                                             aTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCAt                          <  4:151436/141‑1 (MQ=255)
                                                                                                                             aTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAga                  <  4:249968/149‑1 (MQ=255)
                                                                                                                                     tccccacGCCATGACGCTTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACa          >  1:111318/7‑149 (MQ=255)
                                                                                                                                     gcgTATCGCCATGAAGCGTCATGGCGTGGGGAACGCGGGCGGCTTTTGGTAGCATGTTATTGttctttct                                                                                         <  3:443779/70‑1 (MQ=255)
                                                                                                                                     gcgTATCGCCATGAAGCGTCATGGCGTGGGGAACGCGGGCGGCTTTTGGTAGCATGTTATTGttctttct                                                                                         >  4:443779/1‑70 (MQ=255)
                                                                                                                                         cacGCCATGACGCTTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCaa                                            >  4:262256/3‑111 (MQ=255)
                                                                                                                                         cacGCCATGACGCTTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCaa                                            <  3:262256/109‑1 (MQ=255)
                                                                                                                                            gCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATg   <  2:103059/149‑1 (MQ=255)
                                                                                                                                             ccATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATgg  >  4:65454/1‑149 (MQ=255)
                                                                                                                                               aTGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGaa                                                                                  >  2:426449/1‑67 (MQ=255)
                                                                                                                                               aTGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGaa                                                                                  <  1:426449/67‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
TTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGG  >  NC_000913/1928652‑1928941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑