Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,048,920	C→T	8.2%	A1328V (GCG→GTG)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,048,920	0	C	T	8.2%	148.3 / 4.3	49	A1328V (GCG→GTG)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand):  ref base C (24/21);  new base T (2/2);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

AATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCT  >  NC_000913/2048772‑2049067                                                                                                                                                     |                                                                                                                                                    aatGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGc                                                                                                                                                     <  3:409645/149‑1 (MQ=255)  atGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCg                                                                                                                                                    >  4:367794/1‑149 (MQ=255)  atGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCg                                                                                                                                                    <  3:310974/149‑1 (MQ=255)    ggCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCgag                                                                                                                                                  >  2:319329/1‑149 (MQ=255)     gCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCgaga                                                                                                                                                 <  3:51803/149‑1 (MQ=255)                aaaGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCGGGAGCGAGACCGGATACCGt                                                                                                                                      >  4:368218/1‑149 (MQ=255)                 aaGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGtt                                                                                                                                     <  2:219536/149‑1 (MQ=255)                    gtgtGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGtt                                                                                                                                     <  1:316594/146‑1 (MQ=255)                    gtgtGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGtt                                                                                                                                     <  3:393374/146‑1 (MQ=255)                    gtgtGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGtt                                                                                                                                     >  4:393374/1‑146 (MQ=255)                    gtgtGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGtt                                                                                                                                     >  2:316594/1‑146 (MQ=255)                      gtgACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGc                                                                                                                                <  2:434765/149‑1 (MQ=255)                            gtgAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCt                                                                                                                          >  4:163012/1‑149 (MQ=255)                               aaCTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGa                                                                                                                       >  2:362541/1‑149 (MQ=255)                                    cACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGACAAACCGTGACGAACGGACAGGAGAAGGCTACCGTCGCATATACCAATACCCGCTCCTCGATAGAATAAGGAGCGAGACCGGATACCCTTGAGGACAGTCGGGAAAATg                                                                                                                  >  3:294823/1‑149 (MQ=255)                                      ccAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGt                                                                                                                <  2:425149/149‑1 (MQ=255)                                      ccAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGt                                                                                                                <  3:367794/149‑1 (MQ=255)                                       cAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTa                                                                                                               >  2:378633/1‑149 (MQ=255)                                                            aaTGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCaa                                                                                          >  1:24373/1‑149 (MQ=255)                                                                             aaGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGTGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCtt                                                                                                    >  2:224606/1‑122 (MQ=255)                                                                             aaGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGTGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCtt                                                                                                    <  1:224606/122‑1 (MQ=255)                                                                             aaGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGTGAGACCGGATACCGTTGAGCCCAGTCTGGAAAATGGTAGCTCCACGcgt                                                                                                    <  3:304131/122‑3 (MQ=255)                                                                             aaGCCGTGACGAACGAACAGGGTAAGGCTACCCTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGTGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCtt                                                                                                    >  4:304131/1‑122 (MQ=255)                                                                                                 ggTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCAc                                                     >  2:47975/1‑149 (MQ=255)                                                                                                           ccGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAg                                           <  1:319329/149‑1 (MQ=255)                                                                                                              tCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCa                                        >  2:466877/1‑149 (MQ=255)                                                                                                                 cTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACtt                                     <  1:362541/149‑1 (MQ=255)                                                                                                                  ttATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGGGGCCAGGCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACttt                                    >  2:430649/1‑149 (MQ=255)                                                                                                                  ttATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAAt                                                                                     <  4:118406/100‑1 (MQ=255)                                                                                                                  ttATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAAt                                                                                     >  3:118406/1‑100 (MQ=255)                                                                                                                  ttATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGt                                                                     <  2:97633/116‑1 (MQ=255)                                                                                                                  ttATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGt                                                                     >  1:97633/1‑116 (MQ=255)                                                                                                                   tataCCAATACACGCTCCTCGATAGAATCATGAGCGAGACCGGATACCGTTGAGGCTAGTCTGGAAAATGGTAGCTCCATGCTTAGCACATCAATTAATCTCAACGCTGATGCGTCTACGGCACATCTCACTTTGCTACAGGCACtttt                                   <  4:294823/149‑1 (MQ=255)                                                                                                                    ataCCAATACCCGCTCCTCGATAGAATCGGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACttttt                                  <  3:368218/149‑1 (MQ=255)                                                                                                                      aCCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGa                                >  1:267889/1‑149 (MQ=255)                                                                                                                        cAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATa                              >  3:36459/1‑149 (MQ=255)                                                                                                                        cAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATa                              >  2:463640/1‑149 (MQ=255)                                                                                                                        cAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATa                              >  1:301047/1‑149 (MQ=255)                                                                                                                        cAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATa                              >  3:64614/1‑149 (MQ=255)                                                                                                                          aTACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACa                            <  4:49708/149‑1 (MQ=255)                                                                                                                              ccGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGtct                        >  1:244188/1‑149 (MQ=255)                                                                                                                                gCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTcc                      >  4:279418/1‑149 (MQ=255)                                                                                                                                  tcctcGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGc                    <  2:24373/149‑1 (MQ=255)                                                                                                                                    ctcGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGcac                                                            <  4:220263/107‑1 (MQ=255)                                                                                                                                    ctcGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGcac                                                            >  3:220263/1‑107 (MQ=255)                                                                                                                                        aTAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCga              <  3:163012/149‑1 (MQ=255)                                                                                                                                            aaTCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGc                                                                       >  3:357953/1‑88 (MQ=255)                                                                                                                                            aaTCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGc                                                                       <  4:357953/88‑1 (MQ=255)                                                                                                                                              tCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAAc        <  3:218776/149‑1 (MQ=255)                                                                                                                                                    gCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCt  <  2:301047/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    AATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCT  >  NC_000913/2048772‑2049067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑