Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,059,939 G→T 8.8% intergenic (+13/+25) asnU → / ← cbl tRNA‑Asn/ssuEADCB/tauABCD operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,059,9390GT 8.8% 186.2 / 6.2 56intergenic (+13/+25)asnU/cbltRNA‑Asn/ssuEADCB/tauABCD operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (29/22);  new base T (2/3);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTAAAAATCGGGTTTGACAAAAGA‑TTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCT  >  NC_000913/2059794‑2060087
                                                                                                                                                  |                                                                                                                                                    
ttAAAAATCGGGTTTGACAAAAGA‑TTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGccc                                                                                                                                                   >  2:253508/1‑149 (MQ=255)
  aaaaaTCGGGTTTGACAAAAGATTTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCg                                                                                                                                                  <  2:327623/149‑1 (MQ=255)
       tCGGGTTTGACAAAAGA‑TTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTta                                                                                                                                            <  1:217029/149‑1 (MQ=255)
                  aaaaGA‑TTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGAttt                                                                                                                                 >  3:138736/1‑149 (MQ=255)
                   aaaGA‑TTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAATCCCGCTATAAAAGCGGGCtttt                                                                                                                                >  2:21462/1‑149 (MQ=21)
                   aaaGA‑TTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGAtttt                                                                                                                                >  1:66451/1‑149 (MQ=255)
                     aGA‑TTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAATCCCGCTATAAAAGCGGGCttttca                                                                                                                              <  2:388108/149‑3 (MQ=18)
                     aGA‑TTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAATCCCGCTATAAAAGCGGGCttttca                                                                                                                              <  1:21462/149‑3 (MQ=21)
                         tttttCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCta                                                                                                                           >  3:187577/1‑149 (MQ=255)
                               gCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAATCCCGCTATAAAAGCGGGCttttca                                                                                                                              >  1:109541/1‑138 (MQ=18)
                               gCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAATCCCGCTATAAAAGCGGGCttttca                                                                                                                              <  2:109541/140‑3 (MQ=18)
                                           tgCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTc                                                                                                         <  1:253508/149‑1 (MQ=255)
                                             ccTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGTGCCAAATTCCTGAAAAGCCCGCtt                                                                                                                                               <  1:456711/109‑1 (MQ=255)
                                             ccTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCtt                                                                                                                                               >  2:456711/1‑109 (MQ=255)
                                               tCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCctct                                                                                                     >  1:158007/1‑149 (MQ=255)
                                               tCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCctct                                                                                                     >  4:304458/1‑149 (MQ=255)
                                               tCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCctct                                                                                                     >  3:389850/1‑149 (MQ=255)
                                                caacaaCGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCtt                                                                                                    <  4:138736/149‑1 (MQ=255)
                                                  acaacGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCa                                                                                                  >  4:415579/1‑149 (MQ=255)
                                                          tCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTc                                                                                          >  3:79755/1‑149 (MQ=255)
                                                             tctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCAt                                                                                       <  1:233243/149‑1 (MQ=255)
                                                             tctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCAt                                                                                       >  1:412468/1‑149 (MQ=255)
                                                               tGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCa                                                                                     >  2:195742/1‑149 (MQ=255)
                                                                          cGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTg                                                                          <  2:158007/149‑1 (MQ=255)
                                                                          cGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTg                                                                          >  3:424426/1‑149 (MQ=255)
                                                                          cGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTg                                                                          <  3:304458/149‑1 (MQ=255)
                                                                             tAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGata                                                                       >  1:33107/1‑149 (MQ=255)
                                                                               gAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATc                                                                     <  3:415579/149‑1 (MQ=255)
                                                                                 aCGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCt                                                                   <  2:412468/149‑1 (MQ=255)
                                                                                        aCTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGa                                                            >  3:3077/1‑149 (MQ=255)
                                                                                        aCTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGa                                                            <  4:424426/149‑1 (MQ=255)
                                                                                                      tGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTAc                                              >  4:24682/1‑149 (MQ=255)
                                                                                                         cACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACaaa                                           <  1:195742/149‑1 (MQ=255)
                                                                                                          aCTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAg                                          >  1:63561/1‑149 (MQ=255)
                                                                                                           cTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCctc                                                                                                      >  4:476070/1‑88 (MQ=255)
                                                                                                           cTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCctc                                                                                                      <  3:476070/88‑1 (MQ=255)
                                                                                                               ttCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACaa                                            >  3:122253/1‑142 (MQ=255)
                                                                                                               ttCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACaa                                            <  4:122253/142‑1 (MQ=255)
                                                                                                               ttCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCa                                     >  4:375290/1‑149 (MQ=255)
                                                                                                                       cAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcgc                             >  3:144874/1‑149 (MQ=255)
                                                                                                                       cAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcgc                             <  3:442008/149‑1 (MQ=255)
                                                                                                                          tCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAg                          >  2:53214/1‑149 (MQ=255)
                                                                                                                          tCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAg                          >  3:97346/1‑149 (MQ=255)
                                                                                                                              aggagCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCTCTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCAtt                                                <  2:141734/123‑1 (MQ=255)
                                                                                                                              aggagCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCAtt                                                >  1:141734/1‑123 (MQ=255)
                                                                                                                                gagCCAAATTCCTGGAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAg                    >  3:223695/1‑149 (MQ=255)
                                                                                                                                 agCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGt                   <  2:168263/149‑1 (MQ=255)
                                                                                                                                    cAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTAc                <  4:3077/149‑1 (MQ=255)
                                                                                                                                     aaaTTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACg               >  3:92741/1‑149 (MQ=255)
                                                                                                                                        ttCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTg            >  1:220696/1‑149 (MQ=255)
                                                                                                                                          ccTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGaa          <  4:187577/149‑1 (MQ=255)
                                                                                                                                          ccTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGaa          <  2:66451/149‑1 (MQ=255)
                                                                                                                                          ccTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGaa          <  4:389850/149‑1 (MQ=255)
                                                                                                                                          ccTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGCTCCAGAAAGCGCCAGACATAGTTACGCTGaa          >  2:471661/1‑149 (MQ=255)
                                                                                                                                           cTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAg         >  4:224954/1‑149 (MQ=255)
                                                                                                                                                aaGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTc    <  3:224954/149‑1 (MQ=255)
                                                                                                                                                  gCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGCTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCt  <  1:471661/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TTAAAAATCGGGTTTGACAAAAGA‑TTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCT  >  NC_000913/2059794‑2060087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑