Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,532,275 T→A 18.8% intergenic (+51/‑134) cysZ → / → cysK sulfate transporter, sulfite inhibited/cysteine synthase A, O‑acetylserine sulfhydrolase A subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,532,2750TA18.8% 115.0 / 22.3 48intergenic (+51/‑134)cysZ/cysKsulfate transporter, sulfite inhibited/cysteine synthase A, O‑acetylserine sulfhydrolase A subunit
Reads supporting (aligned to +/- strand):  ref base T (22/17);  new base A (4/5);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

GCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTT  >  NC_000913/2532131‑2532423
                                                                                                                                                |                                                                                                                                                    
gctgctTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATgccg                                                                                                                                                  >  2:56120/1‑149 (MQ=255)
     ttAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGCGGCATAAGCCACCCCt                                                                                                                                             <  2:159273/149‑1 (MQ=255)
       aaTCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGCGGCATAAGCCACCCCtgt                                                                                                                                           <  2:414085/149‑3 (MQ=255)
       aaTCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGCGGCATAAGCCACCCCtgt                                                                                                                                           <  2:223000/149‑3 (MQ=255)
                atcatGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCtt                                                                                                                                  >  2:47143/1‑149 (MQ=255)
                atcatGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCtt                                                                                                                                  >  2:161237/1‑149 (MQ=255)
                       ccGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTc                                                                                                                           <  2:192687/149‑1 (MQ=255)
                          ttGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCatt                                                                                                                        <  1:56120/149‑1 (MQ=255)
                              cGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTAttt                                                                                                                    <  1:234951/149‑1 (MQ=255)
                                      gcgcGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTg                                                                                                            <  3:367312/149‑1 (MQ=255)
                                       cgcgACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGCGGCATAAGCCACCCCt                                                                                                                                             >  4:265214/1‑115 (MQ=255)
                                       cgcgACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGCGGCATAAGCCACCCCt                                                                                                                                             <  3:265214/115‑1 (MQ=255)
                                           aCGGCGATGTTGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTGACAATCTACCGGTTATTTTGTAAACGGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGtatat                                                                                                       >  2:432521/1‑149 (MQ=255)
                                                       gTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATg                                                                                                >  3:472008/1‑144 (MQ=255)
                                                       gTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATg                                                                                                <  4:472008/144‑1 (MQ=255)
                                                           aTTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCt                                                                                       >  4:173055/1‑149 (MQ=255)
                                                                      gATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCat                                                                            >  3:57681/1‑149 (MQ=255)
                                                                      gATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCat                                                                            >  3:228304/1‑149 (MQ=255)
                                                                             acGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGata                                                                                                  >  3:325507/1‑120 (MQ=255)
                                                                             acGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGata                                                                                                  <  4:325507/120‑1 (MQ=255)
                                                                             acGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTctct                                                                     >  1:332996/1‑149 (MQ=255)
                                                                               gcgATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGa                                                                   <  3:238292/149‑1 (MQ=255)
                                                                                   tgtgGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCgg                                                               <  1:161237/149‑1 (MQ=255)
                                                                                   tgtgGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCgg                                                               <  1:47143/149‑1 (MQ=255)
                                                                                                   tACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCtgttgt                                               >  1:143592/1‑149 (MQ=255)
                                                                                                            ttttGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGccc                                                                                                                                               >  1:331172/1‑44 (MQ=255)
                                                                                                            ttttGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGccc                                                                                                                                               <  2:331172/44‑1 (MQ=255)
                                                                                                            ttttGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAAtt                                      >  3:266921/1‑149 (MQ=255)
                                                                                                               tGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCa                                   <  2:381150/149‑1 (MQ=255)
                                                                                                                   aaCCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACa                               >  4:89138/1‑149 (MQ=255)
                                                                                                                    aCCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAg                              >  3:17856/1‑149 (MQ=255)
                                                                                                                      cGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGtt                            >  3:237243/1‑149 (MQ=255)
                                                                                                                              tgAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCa                                   <  2:240882/134‑1 (MQ=255)
                                                                                                                              tgAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCa                                   >  1:240882/1‑134 (MQ=255)
                                                                                                                              tgAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAg                    <  3:173055/149‑1 (MQ=255)
                                                                                                                               gAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAgg                   >  2:373896/1‑149 (MQ=255)
                                                                                                                               gAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAgg                   >  3:101651/1‑149 (MQ=255)
                                                                                                                               gAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAgg                   >  1:220142/1‑149 (MQ=255)
                                                                                                                                  aCAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAg                         <  3:43471/140‑1 (MQ=255)
                                                                                                                                  aCAGGGGTGGCATATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCAGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAg                         >  4:43471/1‑140 (MQ=255)
                                                                                                                                   cAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCAt               <  4:237243/149‑1 (MQ=255)
                                                                                                                                   cAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCAt               <  4:266921/149‑1 (MQ=255)
                                                                                                                                   aaGGGGCGGCATAAGCCACCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCAt               >  2:249304/2‑149 (MQ=255)
                                                                                                                                    aGGGGCGGCATAAGCCACCCCTTATTCCATCTTGCATGTCATTATTTCACTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCCGTTGTATCCCAATTTCATACAGTTAAGGACAGACCATg              >  1:162653/1‑149 (MQ=255)
                                                                                                                                     ggggTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGa             >  2:43166/1‑149 (MQ=255)
                                                                                                                                     ggggCGGCATAAGCCACCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACa                               >  1:304845/1‑131 (MQ=255)
                                                                                                                                     ggggCGGCATAAGCCACCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACa                               <  2:304845/131‑1 (MQ=255)
                                                                                                                                               aTGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGAtttt   <  3:375705/149‑1 (MQ=255)
                                                                                                                                                tGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGAttttt  <  4:101651/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTT  >  NC_000913/2532131‑2532423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑