Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,578,180 C→G 9.1% L66L (CTG→CTC)  maeB ← malic enzyme: putative oxidoreductase/phosphotransacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,578,1800CG 9.1% 145.7 / 5.2 44L66L (CTG→CTC) maeBmalic enzyme: putative oxidoreductase/phosphotransacetylase
Reads supporting (aligned to +/- strand):  ref base C (23/17);  new base G (2/2);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

TCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAACCTGGATTTTCCC  >  NC_000913/2578034‑2578323
                                                                                                                                                  |                                                                                                                                                
tCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAg                                                                                                                                                <  1:103857/149‑1 (MQ=255)
                                  tCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAAc                                                                                                             >  3:280692/1‑149 (MQ=255)
                                  tCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAAc                                                                                                             <  3:279634/149‑1 (MQ=255)
                                   cccGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACg                                                                                                            >  3:267527/1‑149 (MQ=255)
                                      ggCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGt                                                                                                         >  4:55088/1‑149 (MQ=255)
                                                   aaCAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTc                                                                                            <  3:243744/149‑1 (MQ=255)
                                                    aCAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTa                                                                                                                       >  4:153402/1‑121 (MQ=255)
                                                    aCAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTa                                                                                                                       <  3:153402/121‑1 (MQ=255)
                                                    aCAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCa                                                                                           >  4:59285/1‑149 (MQ=255)
                                                        aaCGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGAc                                                                                       >  2:231656/1‑149 (MQ=255)
                                                        aaCGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGAc                                                                                       >  2:426086/1‑149 (MQ=255)
                                                           gCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAg                                                                                    <  4:280692/149‑1 (MQ=255)
                                                             ccTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTTGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGt                                                                                  >  4:106892/1‑149 (MQ=255)
                                                             ccTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGt                                                                                  <  3:89772/149‑1 (MQ=255)
                                                                 gCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCgg                                                                              <  2:170439/149‑1 (MQ=255)
                                                                    ttCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCaa                                                                           >  3:153500/1‑149 (MQ=255)
                                                                           aCCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTgg                                                                    >  2:462567/1‑149 (MQ=255)
                                                                                          aGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCCTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAgcgc                                                     >  2:391686/1‑149 (MQ=255)
                                                                                                ccAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATc                                               >  4:373980/1‑149 (MQ=255)
                                                                                                 cAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATcg                                              >  1:472374/1‑149 (MQ=255)
                                                                                                      ttGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCGATCTGGCGCtg                                         <  2:453435/149‑1 (MQ=255)
                                                                                                          cTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGtt                                     >  3:474248/1‑149 (MQ=255)
                                                                                                           tAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGAttt                                                                                             <  4:310217/92‑1 (MQ=255)
                                                                                                           tAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGAttt                                                                                             >  3:310217/1‑92 (MQ=255)
                                                                                                              ccccAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCNCGCTGTGTTGCCa                                 <  1:426086/149‑1 (MQ=255)
                                                                                                                ccAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCaga                               <  1:462567/149‑1 (MQ=255)
                                                                                                                 cAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCagag                              >  1:277628/1‑149 (MQ=255)
                                                                                                                         ccgTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCttggtt                      <  1:231656/149‑1 (MQ=255)
                                                                                                                           gTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCaa                                                                                          <  4:246306/79‑1 (MQ=255)
                                                                                                                           gTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCaa                                                                                          >  3:246306/1‑79 (MQ=255)
                                                                                                                             aCCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGc                                                                                <  1:127451/87‑1 (MQ=255)
                                                                                                                             aCCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGc                                                                                >  2:127451/1‑87 (MQ=255)
                                                                                                                                 ttAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAAc              <  4:267527/149‑1 (MQ=255)
                                                                                                                                 ttAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAAc              >  2:320232/1‑149 (MQ=255)
                                                                                                                                 ttAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAAc              >  2:26083/1‑149 (MQ=255)
                                                                                                                                 tacaaatatACCGCC‑CGAGGTAACCTGGTGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGtt                                     >  3:320824‑M1/10‑126 (MQ=255)
                                                                                                                                 tacaaatatACCGCC‑CGAGGTAACCTGGTGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGtt                                     <  4:320824‑M1/117‑1 (MQ=255)
                                                                                                                                    gagaTCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTa                                                                                                                       >  4:238536/1‑41 (MQ=255)
                                                                                                                                    gagaTCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTa                                                                                                                       <  3:238536/41‑1 (MQ=255)
                                                                                                                                      atatACCGCC‑CGAGGTAACCTGGTGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAg                                                             <  1:351321‑M1/93‑1 (MQ=255)
                                                                                                                                      atatACCGCC‑CGAGGTAACCTGGTGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAg                                                             >  2:351321‑M1/5‑97 (MQ=255)
                                                                                                                                         cACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAACCTGGAttt      >  2:472352/1‑149 (MQ=255)
                                                                                                                                          ACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAACCTGGAtttt     <  3:454969‑M1/149‑1 (MQ=255)
                                                                                                                                             GCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAACCTGGATTTTccc  >  4:153500‑M1/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAACCTGGATTTTCCC  >  NC_000913/2578034‑2578323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑