Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,578,185 T→A 9.1% N65Y (AAC→TAC)  maeB ← malic enzyme: putative oxidoreductase/phosphotransacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,578,1850TA 9.1% 142.7 / 5.2 44N65Y (AAC→TAC) maeBmalic enzyme: putative oxidoreductase/phosphotransacetylase
Reads supporting (aligned to +/- strand):  ref base T (24/16);  new base A (2/2);  total (26/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

TCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAACCTGGATTTTCCCTGGAACT  >  NC_000913/2578068‑2578330
                                                                                                                     |                                                                                                                                                  
tCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAAc                                                                                                                    >  3:280692/1‑149 (MQ=255)
tCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAAc                                                                                                                    <  3:279634/149‑1 (MQ=255)
 cccGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACg                                                                                                                   >  3:267527/1‑149 (MQ=255)
    ggCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGt                                                                                                                >  4:55088/1‑149 (MQ=255)
                 aaCAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTc                                                                                                   <  3:243744/149‑1 (MQ=255)
                  aCAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTa                                                                                                                              >  4:153402/1‑121 (MQ=255)
                  aCAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTa                                                                                                                              <  3:153402/121‑1 (MQ=255)
                  aCAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCa                                                                                                  >  4:59285/1‑149 (MQ=255)
                      aaCGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGAc                                                                                              >  2:231656/1‑149 (MQ=255)
                      aaCGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGAc                                                                                              >  2:426086/1‑149 (MQ=255)
                         gCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAg                                                                                           <  4:280692/149‑1 (MQ=255)
                           ccTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTTGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGt                                                                                         >  4:106892/1‑149 (MQ=255)
                           ccTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGt                                                                                         <  3:89772/149‑1 (MQ=255)
                               gCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCgg                                                                                     <  2:170439/149‑1 (MQ=255)
                                  ttCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCaa                                                                                  >  3:153500/1‑149 (MQ=255)
                                         aCCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTgg                                                                           >  2:462567/1‑149 (MQ=255)
                                                        aGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCCTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAgcgc                                                            >  2:391686/1‑149 (MQ=255)
                                                              ccAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATc                                                      >  4:373980/1‑149 (MQ=255)
                                                               cAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATcg                                                     >  1:472374/1‑149 (MQ=255)
                                                                    ttGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCGATCTGGCGCtg                                                <  2:453435/149‑1 (MQ=255)
                                                                        cTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGtt                                            >  3:474248/1‑149 (MQ=255)
                                                                         tAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGAttt                                                                                                    <  4:310217/92‑1 (MQ=255)
                                                                         tAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGAttt                                                                                                    >  3:310217/1‑92 (MQ=255)
                                                                            ccccAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCNCGCTGTGTTGCCa                                        <  1:426086/149‑1 (MQ=255)
                                                                              ccAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCaga                                      <  1:462567/149‑1 (MQ=255)
                                                                               cAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCagag                                     >  1:277628/1‑149 (MQ=255)
                                                                                       ccgTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCttggtt                             <  1:231656/149‑1 (MQ=255)
                                                                                         gTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCaa                                                                                                 >  3:246306/1‑79 (MQ=255)
                                                                                         gTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCaa                                                                                                 <  4:246306/79‑1 (MQ=255)
                                                                                           aCCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGc                                                                                       <  1:127451/87‑1 (MQ=255)
                                                                                           aCCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGc                                                                                       >  2:127451/1‑87 (MQ=255)
                                                                                               ttAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAAc                     <  4:267527/149‑1 (MQ=255)
                                                                                               ttAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAAc                     >  2:320232/1‑149 (MQ=255)
                                                                                               ttAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAAc                     >  2:26083/1‑149 (MQ=255)
                                                                                               tacaaatatACCGCC‑CGAGGTAACCTGGTGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGtt                                            <  4:320824‑M1/117‑1 (MQ=255)
                                                                                               tacaaatatACCGCC‑CGAGGTAACCTGGTGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGtt                                            >  3:320824‑M1/10‑126 (MQ=255)
                                                                                                  gagaTCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTa                                                                                                                              >  4:238536/1‑41 (MQ=255)
                                                                                                  gagaTCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTa                                                                                                                              <  3:238536/41‑1 (MQ=255)
                                                                                                    atatACCGCC‑CGAGGTAACCTGGTGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAg                                                                    >  2:351321‑M1/5‑97 (MQ=255)
                                                                                                    atatACCGCC‑CGAGGTAACCTGGTGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAg                                                                    <  1:351321‑M1/93‑1 (MQ=255)
                                                                                                       cACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAACCTGGAttt             >  2:472352/1‑149 (MQ=255)
                                                                                                        ACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAACCTGGAtttt            <  3:454969‑M1/149‑1 (MQ=255)
                                                                                                           GCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAACCTGGATTTTccc         >  4:153500‑M1/1‑149 (MQ=255)
                                                                                                                  ggTTAACGCG‑GGCGGTATATGTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAACCTGGATTTTCCctggaact  >  4:315475/1‑149 (MQ=255)
                                                                                                                     |                                                                                                                                                  
TCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAACCTGGATTTTCCCTGGAACT  >  NC_000913/2578068‑2578330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑