Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,724,099 T→G 9.1% intergenic (+17/‑29) pssA → / → yfiM phosphatidylserine synthase; CDP‑diacylglycerol‑serine O‑phosphatidyltransferase/putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,724,0990TG 9.1% 181.4 / 11.0 55intergenic (+17/‑29)pssA/yfiMphosphatidylserine synthase; CDP‑diacylglycerol‑serine O‑phosphatidyltransferase/putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (23/27);  new base G (3/2);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01

CGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCG  >  NC_000913/2723951‑2724235
                                                                                                                                                    |                                                                                                                                        
cgcgAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGt                                                                                                                                          >  4:387049/1‑149 (MQ=255)
    aGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACgg                                                                                                                                      <  1:180622/149‑1 (MQ=255)
      cATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACgggg                                                                                                                                    <  3:267504/149‑1 (MQ=255)
         accaccATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTACAGGACGGGgttg                                                                                                                                 <  1:122467/149‑2 (MQ=255)
         accaccATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTACAGGACGGGgttg                                                                                                                                 <  1:172082/149‑2 (MQ=255)
            accaTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgtttgtt                                                                                                                              >  2:28652/1‑149 (MQ=255)
             ccaTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgtttgttt                                                                                                                             <  3:387049/149‑1 (MQ=255)
              caTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGtttt                                                                                                                            <  2:117622/149‑1 (MQ=255)
                tCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGtttttt                                                                                                                          >  4:112377/1‑149 (MQ=255)
                tCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGtttttt                                                                                                                          <  3:373934/149‑1 (MQ=255)
                tCGTTAAACACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGtttttt                                                                                                                          >  3:187971/1‑149 (MQ=255)
                   ttAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGa                                                                                                                       >  1:171753/1‑149 (MQ=255)
                    tAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAg                                                                                                                      <  2:305930/149‑1 (MQ=255)
                     aaGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCAGTACGGGGTTTGTTTTTTg                                                                                                                         <  3:148237/145‑1 (MQ=255)
                     aaGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCAGTACGGGGTTTGTTTTTTg                                                                                                                         >  4:148237/1‑145 (MQ=255)
                       gCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGcc                                                                                                                   >  2:46995/1‑149 (MQ=255)
                           tATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCATTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGt                                                                                                               >  1:250504/1‑149 (MQ=255)
                             tCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGttt                                                                                                             >  3:100750/1‑149 (MQ=255)
                              cgcgATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGtttt                                                                                                            <  3:112377/149‑1 (MQ=255)
                                cgATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGc                                                                                                          <  1:270994/149‑1 (MQ=255)
                                cgATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGc                                                                                                          <  1:135236/149‑1 (MQ=255)
                                      gCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgttt                                                                                                                                 >  2:205630/1‑120 (MQ=255)
                                      gCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgttt                                                                                                                                 <  1:205630/120‑1 (MQ=255)
                                          aGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCtttt                                                                                                <  3:447416/149‑1 (MQ=255)
                                          aGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCtttt                                                                                                <  4:100750/149‑1 (MQ=255)
                                           gTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCttttt                                                                                               <  1:94933/149‑1 (MQ=255)
                                                 ccGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGt                                                                                         >  4:277846/1‑149 (MQ=255)
                                                 ccGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGt                                                                                         >  2:465591/1‑149 (MQ=255)
                                                   gATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACggg                                                                                                                                     >  3:97432/1‑103 (MQ=255)
                                                   gATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACggg                                                                                                                                     <  4:97432/103‑1 (MQ=255)
                                                         ccGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTAtt                                                                                 <  4:187971/149‑1 (MQ=255)
                                                            gTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTCTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTc                                                                                       >  2:313091/1‑140 (MQ=255)
                                                            gTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTCTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTc                                                                                       <  1:313091/140‑1 (MQ=255)
                                                              gAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTAc                                                                            <  1:402325/149‑1 (MQ=255)
                                                                     cGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTgg                                                                     >  1:154431/1‑149 (MQ=255)
                                                                        aaaCTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTAtt                                                                                 >  1:341256/1‑134 (MQ=255)
                                                                        aaaCTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTAtt                                                                                 <  2:341256/134‑1 (MQ=255)
                                                                         aaCTCATCCGCCATTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGc                                                                 <  2:250504/149‑1 (MQ=255)
                                                                          aCTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCa                                                                >  3:424817/1‑149 (MQ=255)
                                                                                 cgccgTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCatat                                                         <  3:277846/149‑1 (MQ=255)
                                                                                  gccgTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATg                                                        <  2:171753/149‑1 (MQ=255)
                                                                                   ccgTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATgg                                                       <  1:46995/149‑1 (MQ=255)
                                                                                      tttGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTa                                                    >  4:329492/1‑149 (MQ=255)
                                                                                                         gACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCa                                 >  1:133084/1‑149 (MQ=255)
                                                                                                                   ttaGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCt                       >  3:440838/1‑149 (MQ=255)
                                                                                                                       ccGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAac                   >  3:266145/1‑149 (MQ=255)
                                                                                                                                gTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAg                                >  2:195397/1‑127 (MQ=255)
                                                                                                                                gTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAg                                <  1:195397/127‑1 (MQ=255)
                                                                                                                                    tCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTc      <  1:465591/149‑1 (MQ=255)
                                                                                                                                      acaACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCgg    >  3:60376/1‑149 (MQ=255)
                                                                                                                                       caACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTACTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCGACACTTTATCGCCTCGGc   <  1:312112/149‑1 (MQ=255)
                                                                                                                                        aaCCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGc                                                      <  3:7471/97‑1 (MQ=255)
                                                                                                                                        aaCCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGc                                                      >  4:7471/1‑97 (MQ=255)
                                                                                                                                        aaCCCCGTACAGGACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCg  >  1:321307/1‑149 (MQ=255)
                                                                                                                                        aaCCCCGTACAGGACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCg  >  3:412788/1‑149 (MQ=255)
                                                                                                                                        aaCCCCGTACAGGACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCg  >  3:408826/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                        
CGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCG  >  NC_000913/2723951‑2724235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑