Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,907,390	G→C	6.0%	R184R (CGC→CGG)	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,907,390	0	G	C	6.0%	233.8 / 6.2	67	R184R (CGC→CGG)	eno	enolase
Reads supporting (aligned to +/- strand):  ref base G (31/32);  new base C (2/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.25e-01

GCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTC  >  NC_000913/2907247‑2907536                                                                                                                                                |                                                                                                                                                   gcTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGAt                                                                                                                                               <  2:20676/149‑1 (MQ=255)   tttAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATgg                                                                                                                                             >  1:114851/1‑149 (MQ=255)          gCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCttt                                                                                                                                      <  4:179024/149‑1 (MQ=255)                gCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGt                                                                                                                                <  1:187833/149‑1 (MQ=255)                 cGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGtt                                                                                                                               >  1:410851/1‑149 (MQ=255)                 cGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGtt                                                                                                                               <  3:306515/149‑1 (MQ=255)                        aGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGcttctt                                                                                                                                       >  4:378182/1‑134 (MQ=255)                        aGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGcttctt                                                                                                                                       <  3:378182/134‑1 (MQ=255)                         gCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGcc                                                                                                                       >  3:267500/1‑149 (MQ=255)                                 ttCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCt                                                                                                               >  4:439529/1‑149 (MQ=255)                                    aGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGACCTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGaa                                                                                                            <  4:109096/149‑1 (MQ=255)                                             aCCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAAtt                                                                                                   >  3:284635/1‑149 (MQ=255)                                                cAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCt                                                                                                >  4:282076/1‑149 (MQ=255)                                                 aGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTca                                                                                                                                    <  1:3984/112‑1 (MQ=255)                                                 aGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTca                                                                                                                                    >  2:3984/1‑112 (MQ=255)                                                 aGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTg                                                                                               <  4:245561/149‑1 (MQ=255)                                                 aGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTg                                                                                               <  3:277401/149‑1 (MQ=255)                                                 aGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTg                                                                                               <  3:15901/149‑1 (MQ=255)                                                   gTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGa                                                                                             >  4:81401/1‑149 (MQ=255)                                                        gcgcATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCa                                                                                        >  2:465210/1‑149 (MQ=255)                                                          gcATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAc                                                                                      >  4:192538/1‑149 (MQ=255)                                                           cATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACg                                                                                     <  1:94880/149‑1 (MQ=255)                                                               gCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACgttgt                                                                                 >  2:58273/1‑149 (MQ=255)                                                                ccACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACgttgtt                                                                                >  3:168294/1‑149 (MQ=255)                                                                  aCCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGaa                                                                                                     >  1:61393/1‑126 (MQ=255)                                                                  aCCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGaa                                                                                                     <  2:61393/126‑1 (MQ=255)                                                                  aCCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTtgttgt                                                                              >  3:341801/1‑149 (MQ=255)                                                                  aCCTTCGGCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCGGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACTGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTtgttgt                                                                              >  4:402583/1‑149 (MQ=255)                                                                               aCAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTcacc                                                                 >  1:76413/1‑149 (MQ=255)                                                                                    agTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGt                                                            <  3:81401/149‑1 (MQ=255)                                                                                     gtgtTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGtt                                                           >  1:22795/1‑149 (MQ=255)                                                                                       gtTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTtga                                                         <  4:326435/149‑1 (MQ=255)                                                                                          cATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTtgatga                                                      <  4:267500/149‑1 (MQ=255)                                                                                           aTGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTgatgat                                                     >  2:270523/1‑149 (MQ=255)                                                                                            tGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGatgatg                                                    <  4:284635/149‑1 (MQ=255)                                                                                             gCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGata                                                                                           >  1:255376/1‑109 (MQ=255)                                                                                             gCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGata                                                                                           <  2:255376/109‑1 (MQ=255)                                                                                                 ttCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTtcat                                               <  2:438341/149‑1 (MQ=255)                                                                                                 ttCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTtcat                                               <  3:282076/149‑1 (MQ=255)                                                                                                   cGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTtcatca                                             >  3:186803/1‑149 (MQ=255)                                                                                                           gAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAAcc                                     >  3:427152/1‑149 (MQ=255)                                                                                                              cTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGc                                  >  1:91801/1‑149 (MQ=255)                                                                                                                 ttGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATa                               <  3:439529/149‑1 (MQ=255)                                                                                                                   gCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATaga                             <  1:58273/149‑1 (MQ=255)                                                                                                                   gCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATaga                             <  3:250259/149‑1 (MQ=255)                                                                                                                   gCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATaga                             <  2:76413/149‑1 (MQ=255)                                                                                                                   gCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATaga                             >  4:439442/1‑149 (MQ=255)                                                                                                                      aGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACCTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTa                          >  2:191660/1‑149 (MQ=255)                                                                                                                       ggTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTAt                         <  2:114851/149‑1 (MQ=255)                                                                                                                         tGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTAttt                       <  2:22795/149‑1 (MQ=255)                                                                                                                            tGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGaa                                                                                                            >  2:272483/1‑61 (MQ=255)                                                                                                                            tGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGaa                                                                                                            <  1:272483/61‑1 (MQ=255)                                                                                                                              gAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCg                  <  2:410851/149‑1 (MQ=255)                                                                                                                                aaaCTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGa                >  1:292971/1‑149 (MQ=255)                                                                                                                                aaaCTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGa                <  1:270523/149‑1 (MQ=255)                                                                                                                                aaaCTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGa                <  3:402583/149‑1 (MQ=255)                                                                                                                                 aaCTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAg               <  2:292971/149‑1 (MQ=255)                                                                                                                                   cTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTa             <  4:168294/149‑1 (MQ=255)                                                                                                                                     tCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTAcc           <  1:465210/149‑1 (MQ=255)                                                                                                                                     aaaGAAGCCATCCGCATGGGTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACgttgtt                                                                                >  1:273792/3‑80 (MQ=37)                                                                                                                                     aaaGAAGCCATCCGCATGGGTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACgttgtt                                                                                <  2:273792/78‑1 (MQ=37)                                                                                                                                       aGAAGCCATCCGCATGGGTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATaga                             <  3:386668/129‑1 (MQ=255)                                                                                                                                       aGAAGCCATCCGCATGGGTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATaga                             >  4:386668/1‑129 (MQ=255)                                                                                                                                        gAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgtt        >  4:11296/1‑149 (MQ=255)                                                                                                                                            ccATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAACTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgttcagt    >  1:225716/1‑149 (MQ=255)                                                                                                                                              aTGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACGGGCTGAATCTTGAATTCCTGGATATCAACGTTGTTGCCGGCTTGCTCACCACCGTGGATGATGTCCACCATCGGAACCGGCATAGAGTTTTTGCCCGGAGTACCgttcagttc  >  2:405608/1‑149 (MQ=255)                                                                                                                                              aTGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgttcagttc  >  3:128501/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                   GCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTC  >  NC_000913/2907247‑2907536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑