Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,999,023 G→C 10.0% noncoding (35/74 nt) glyU ← tRNA‑Gly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,999,0230GC10.0% 166.1 / 8.3 50noncoding (35/74 nt)glyUtRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base G (22/23);  new base C (2/3);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

AACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGCTGCTGACTGCTGATTAATTCGCCATCAGCATTTTGGCTTGCTGCCCTGAGGCGGCAAGT  >  NC_000913/2998880‑2999170
                                                                                                                                               |                                                                                                                                                   
aacTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAg                                                                                                                                                >  1:215874/1‑149 (MQ=255)
   ttGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGctc                                                                                                                                             >  1:30963/1‑149 (MQ=255)
     gAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAGGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCtctc                                                                                                                                           >  4:41857/1‑149 (MQ=255)
     gAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCtctc                                                                                                                                           <  2:115825/149‑1 (MQ=255)
       aaaTAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTCCCAAGctctata                                                                                                                                         <  3:238574/149‑4 (MQ=255)
         aTAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTCCCAAGctctatacg                                                                                                                                       >  2:229550/1‑144 (MQ=255)
           aaGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTa                                                                                                                                     <  3:256625/149‑1 (MQ=255)
           aaGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTa                                                                                                                                     <  4:124949/149‑1 (MQ=255)
           aaGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTa                                                                                                                                     >  1:60786/1‑149 (MQ=255)
            aGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTAc                                                                                                                                    <  3:171278/149‑1 (MQ=255)
            aGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTAc                                                                                                                                    >  3:338806/1‑149 (MQ=255)
             ggTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTAcc                                                                                                                                   >  3:110705/1‑149 (MQ=255)
              gtgtTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTCCCAAGctctatacga                                                                                                                                      >  1:430668/1‑139 (MQ=255)
              gtgtTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTCCCAAGctctatacga                                                                                                                                      <  2:430668/145‑7 (MQ=255)
                                tACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTc                                                                                                                <  4:110705/149‑1 (MQ=255)
                                tACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTc                                                                                                                <  3:15564/149‑1 (MQ=255)
                                  cAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCga                                                                                                              <  2:30963/149‑1 (MQ=255)
                                  cAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCga                                                                                                              <  4:54776/149‑1 (MQ=255)
                                   aaaaGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCgag                                                                                                             >  2:170667/1‑149 (MQ=255)
                                   aaaaGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCgag                                                                                                             >  4:46258/1‑149 (MQ=255)
                                           tgcattgcGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTaa                                                                                                     <  2:60786/149‑1 (MQ=255)
                                            tcattgcGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAGGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAg                                                                                                    <  3:41857/148‑1 (MQ=255)
                                                tgcGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCAt                                                                                                <  4:315416/149‑1 (MQ=255)
                                                 gcGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCAtt                                                                                               >  3:274857/1‑149 (MQ=255)
                                                   gTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGtt                                                                                                                                        <  1:257998/106‑1 (MQ=255)
                                                   gTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGtt                                                                                                                                        >  2:257998/1‑106 (MQ=255)
                                                       aCGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAAc                                                                                         >  4:19459/1‑149 (MQ=255)
                                                                       acaaAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATACGTAAGGCATTATAAACCTTACGCTCTCCt                                                                            >  1:464729/1‑146 (MQ=255)
                                                                       acaaAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATACGTAAGGCATTATAAACCTTACGCTCTCCt                                                                            <  2:464729/146‑1 (MQ=255)
                                                                            aTGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGt                                                                    <  2:215874/149‑1 (MQ=255)
                                                                                   aGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACgc                                                             <  4:333211/149‑1 (MQ=255)
                                                                                    gTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACgct                                                            >  4:385980/1‑149 (MQ=255)
                                                                                      aTTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACgctgc                                                          <  4:331173/149‑1 (MQ=255)
                                                                                       ttACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTAGAATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACgctgct                                                         >  4:396271/1‑149 (MQ=255)
                                                                                              gAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGctgctgactgct                                                  >  4:128086/1‑149 (MQ=255)
                                                                                                  aTAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGctgctgactgctga                                                <  2:116865/147‑1 (MQ=255)
                                                                                                  aTAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGctgctgactgctga                                                >  1:116865/1‑147 (MQ=255)
                                                                                                    aaaTTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGCTGCTGACTGCTGATTaa                                            <  4:338806/149‑1 (MQ=255)
                                                                                                      aTTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGCTGCTGACTGCTGATTAAtt                                          <  3:19459/149‑1 (MQ=255)
                                                                                                                        aaTCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCa                                                                                                 >  3:299179/1‑76 (MQ=255)
                                                                                                                        aaTCGAACCCTCGTATAGAGCTTGGGAAACTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCa                                                                                                 <  4:299179/76‑1 (MQ=255)
                                                                                                                           cGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGCTGCTGACTGCTGATTAATTCGCCATCAGCATTTTGGCTtg                     <  3:128086/149‑1 (MQ=255)
                                                                                                                                ccTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGCTGCTGACTGCTGATTAATTCGCCATCAGCATTTTGGCTTGCTGcc                >  4:441203/1‑149 (MQ=255)
                                                                                                                                ccTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGCTGCTGACTGCTGATTAATTCGCCATCAGCATTTTGGCTTGCTGcc                <  3:46258/149‑1 (MQ=255)
                                                                                                                                  tCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGCTGCTGACTGCTGATTAATTCGCCATCAGCATTTTGGCTTGCTGCCCt              >  3:368928/1‑149 (MQ=255)
                                                                                                                                     acgagagCTTCCCAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGCTGCTGACTGCTGAt                                               <  4:56517/110‑1 (MQ=255)
                                                                                                                                        agagCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGc                                                                                                         <  4:12892/52‑1 (MQ=255)
                                                                                                                                        agagCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGc                                                                                                         >  3:12892/1‑52 (MQ=255)
                                                                                                                                           gCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGCTGCTGACTGCTGATTAATTTGCCATCAGCATTTTGGCTTGCTGCCCTGAGGCGGCa     >  2:4663/1‑149 (MQ=255)
                                                                                                                                              tGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGCTGCTGACTGCTGATTAATTCGCCATCAGCATTTTGGCTTGCTGCCCTGAGGCGGCAAGt  >  3:464989/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
AACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGCTGCTGACTGCTGATTAATTCGCCATCAGCATTTTGGCTTGCTGCCCTGAGGCGGCAAGT  >  NC_000913/2998880‑2999170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑