Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,334,703	A→G	7.5%	intergenic (+22/‑206)	ispB → / → sfsB	octaprenyl diphosphate synthase/malPQ operon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,334,703	0	A	G	7.5%	162.7 / 4.1	54	intergenic (+22/‑206)	ispB/sfsB	octaprenyl diphosphate synthase/malPQ operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (27/22);  new base G (2/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.57e-01

CGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTG  >  NC_000913/3334562‑3334851                                                                                                                                              |                                                                                                                                                      cgTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGc‑gcgcg                                                                                                                                               <  4:303441/149‑1 (MQ=255)      gCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATg                                                                                                                                          >  1:374238/1‑149 (MQ=255)       cGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATgg                                                                                                                                         <  3:273398/149‑1 (MQ=255)        gTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGt                                                                                                                                        >  1:236708/1‑149 (MQ=255)                gaagaaGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTaa                                                                                                                                <  1:9621/149‑1 (MQ=255)                     agCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTc                                                                                                                            >  2:309767/1‑148 (MQ=255)                     agCAGACAAAGCCATCGCAGCGTTACAGGAGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTc                                                                                                                            <  1:309767/148‑1 (MQ=255)                        aGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATa                                                                                                                        >  2:419244/1‑149 (MQ=255)                        aGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATa                                                                                                                        >  1:51521/1‑149 (MQ=255)                         gACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGtt                                                                                                                                       >  3:213928/1‑133 (MQ=255)                         gACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGtt                                                                                                                                       <  4:213928/133‑1 (MQ=255)                            aaaGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATaaaaa                                                                                                                    <  2:437282/149‑1 (MQ=255)                               gCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAacac                                                                                                                 <  1:24598/149‑1 (MQ=255)                               gCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAacac                                                                                                                 >  1:322825/1‑149 (MQ=255)                               gCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCCCATCCCGCGCGCT‑GCGCGGGATgag                                                                                                                                        >  1:302667/1‑124 (MQ=255)                               gCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCCCATCCCGCGC‑GCTGCGCGGGATgag                                                                                                                                        <  2:302667/126‑3 (MQ=255)                                      cAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCa                                                                                                          >  1:472867/1‑149 (MQ=255)                                       aGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCCCATCCCGCGCGCT‑GCGCgg                                                                                                                                              <  4:320437/112‑1 (MQ=255)                                          gTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGCT‑GCGCGGGa                                                                                                                                            <  1:402967/111‑1 (MQ=255)                                          gTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGCT‑GCGCGGGa                                                                                                                                            >  2:402967/1‑111 (MQ=255)                                           ttACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATaaaaa                                                                                                                    <  2:4121/134‑1 (MQ=255)                                           ttACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATaaaaa                                                                                                                    >  1:4121/1‑134 (MQ=255)                                               aGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATaa                                                                                                 >  2:98354/1‑149 (MQ=255)                                                  tGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAacac                                                                                              <  2:472867/149‑1 (MQ=255)                                                             acCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAg                                                                                   <  1:98354/149‑1 (MQ=255)                                                               cccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAgat                                                                                 >  3:216530/1‑147 (MQ=255)                                                                ccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGa                                                                                >  1:98307/1‑149 (MQ=255)                                                                       gagaAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGtt                                                                                                                                       >  2:381568/1‑87 (MQ=255)                                                                       gagaAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGtt                                                                                                                                       <  1:381568/87‑1 (MQ=255)                                                                         gaAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAg                                                                                   <  3:119830/137‑1 (MQ=255)                                                                         gaAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAg                                                                                   >  4:119830/1‑137 (MQ=255)                                                                           aGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAtgt                                                                             >  3:195438/1‑141 (MQ=255)                                                                           aGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAtgt                                                                             <  4:195438/141‑1 (MQ=255)                                                                             cACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtat                                                                   >  1:355771/1‑149 (MQ=255)                                                                              aCTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtata                                                                  >  2:40697/1‑149 (MQ=255)                                                                              aCTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtata                                                                  >  1:235103/1‑149 (MQ=255)                                                                                tCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtatata                                                                >  4:144419/1‑149 (MQ=255)                                                                                  aTCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATAtt                                                              <  1:419244/149‑1 (MQ=255)                                                                                        cTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATGTATTCTGAat                                                        >  4:265353/1‑149 (MQ=255)                                                                                         tCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAata                                                       <  2:374238/149‑1 (MQ=255)                                                                                           gcgcACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATAtt                                                     >  1:218772/1‑149 (MQ=255)                                                                                                  tCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACActc                                              <  3:144419/149‑1 (MQ=255)                                                                                                           aaCGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTcaca                                                 <  3:149201/137‑1 (MQ=255)                                                                                                           aaCGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTcaca                                                 >  4:149201/1‑137 (MQ=255)                                                                                                           aaCGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGa                                     >  3:366365/1‑149 (MQ=255)                                                                                                               cgATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACtt                                 <  2:355771/149‑1 (MQ=255)                                                                                                               cgATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACtt                                 <  4:366365/149‑1 (MQ=255)                                                                                                                gATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACttt                                <  2:236708/149‑1 (MQ=255)                                                                                                                       aaTCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGc                         <  2:98307/149‑1 (MQ=255)                                                                                                                            cctcccctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAg                    >  3:147661/1‑149 (MQ=255)                                                                                                                            cctcccctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAg                    >  2:465457/1‑149 (MQ=255)                                                                                                                                      tCCCGCGCAGC‑GCGCGGGATGGCTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACGCTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa          <  2:235103/149‑1 (MQ=255)                                                                                                                                         cgcgCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGta       >  3:303973/1‑149 (MQ=255)                                                                                                                                         cgcgCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGta       >  1:10449/1‑149 (MQ=255)                                                                                                                                              aGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTg  >  1:425231/1‑149 (MQ=255)                                                                                                                                              |                                                                                                                                                      CGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTG  >  NC_000913/3334562‑3334851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑