| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,477,878 | T→A | 24.2% | intergenic (+20/+29) | slyX → / ← slyD | phi X174 lysis protein/FKBP‑type peptidyl prolyl cis‑trans isomerase (rotamase) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,477,878 | 0 | T | A | 24.2% | 65.7 / 18.6 | 33 | intergenic (+20/+29) | slyX/slyD | phi X174 lysis protein/FKBP‑type peptidyl prolyl cis‑trans isomerase (rotamase) |
| Reads supporting (aligned to +/- strand): ref base T (13/12); new base A (5/3); total (18/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01 | |||||||||||
ACCGCTCATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCGTGGTG > NC_000913/3477733‑3478023 | aCCGCTCATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTcc < 2:137241/149‑1 (MQ=255) cGCTCATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTcccc < 1:116476/149‑1 (MQ=255) cGCTCATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTcccc < 1:273187/149‑1 (MQ=255) aTGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGC‑ttt > 2:151500/1‑149 (MQ=255) aTGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGC‑ttt < 2:350401/149‑1 (MQ=255) tGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGC‑tttt > 2:355533/1‑149 (MQ=255) aCTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGC‑TTTTTTGTCACTTTTTCGGTAt < 1:175476/149‑1 (MQ=255) tgctgACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAAccgcc > 4:433296/1‑149 (MQ=255) tgctgACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAAccgcc > 2:359475/1‑149 (MQ=255) cGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAtt > 4:72021/1‑94 (MQ=255) cGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAtt < 3:72021/94‑1 (MQ=255) gagaAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGC‑TTTTTTGTCACTTTTTCGGTATTa < 4:150302/123‑1 (MQ=255) gagaAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGC‑TTTTTTGTCACTTTTTCGGTATTa > 3:150302/1‑123 (MQ=255) gTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCg > 4:322587/1‑149 (MQ=255) agccagccagccGTCGAACATCGCATCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCAcag > 4:335512/1‑149 (MQ=255) agccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGt > 4:191869/1‑124 (MQ=255) agccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGt < 3:191869/124‑1 (MQ=255) ccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGC‑ttttt > 1:90883/1‑83 (MQ=255) ccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGC‑ttttt < 2:90883/83‑1 (MQ=255) cATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGC‑TTTTTTGTCACTTTTTCAGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCgtgt < 4:312288/149‑1 (MQ=255) tGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCg > 3:344003/1‑149 (MQ=255) aagaaACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTg < 3:25997/149‑1 (MQ=255) agaaACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTgg < 1:151500/149‑1 (MQ=255) agaaACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTgg < 1:359475/149‑1 (MQ=255) gaaACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGc > 4:238568/1‑149 (MQ=255) aaaaaaGCGGGGATTCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCgtcgtggtcgt > 1:411464/1‑149 (MQ=255) aaaaaaGCGGGGAATCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCgtcgtggtcgt > 4:433653/1‑149 (MQ=255) aaaaaaGCGGGGAATCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCgtcgtggtcgt > 1:296609/1‑149 (MQ=255) aaaaaaGCGGGGAATCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCgtcgtggtcat > 2:38245/1‑147 (MQ=255) aaaaaGCGGGGAATCCCCGCTTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCgtcgtggtcgt > 1:84922/1‑149 (MQ=255) aaaaGCGGGGAATCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGa > 3:2996/1‑149 (MQ=255) aaGCGGGGATTCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATc < 4:138048/149‑1 (MQ=255) aGCGGGGATTCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCg > 1:455305/1‑149 (MQ=255) gggAATCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCATGATCgtggt < 1:38245/149‑1 (MQ=255) ggAATCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCgtggtg < 2:296609/149‑1 (MQ=255) | ACCGCTCATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGC‑TTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCGTGGTG > NC_000913/3477733‑3478023 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |