| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,639,344 | T→A | 14.7% | intergenic (+203/+41) | pitA → / ← uspB | phosphate transporter, low‑affinity; tellurite importer/universal stress (ethanol tolerance) protein B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,639,344 | 0 | T | A | 14.7% | 100.7 / 9.5 | 34 | intergenic (+203/+41) | pitA/uspB | phosphate transporter, low‑affinity; tellurite importer/universal stress (ethanol tolerance) protein B |
| Reads supporting (aligned to +/- strand): ref base T (14/15); new base A (2/3); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01 | |||||||||||
GCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCA > NC_000913/3639196‑3639490 | gcgcTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGttt > 4:405987/1‑149 (MQ=255) tttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTg > 3:137989/1‑149 (MQ=255) cATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAACACATTGCAGAGAGTATGTAGGCCTGATAAGCGTAGCGCATCAGGGAACTTTGCGTTTGTAATCAGTCTCGAGCGGGCCAGTAAACTGACCCGc < 2:186172/149‑1 (MQ=18) ggATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCtttttt < 3:405987/149‑1 (MQ=255) tGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTAt < 3:230317/149‑1 (MQ=255) cggcgTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGc < 3:16752/149‑1 (MQ=255) aaCGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGttatta < 4:315290/149‑1 (MQ=255) cGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTattatt < 4:428683/149‑1 (MQ=255) ggCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGc > 3:307219/1‑149 (MQ=255) tACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTaa < 4:137989/149‑1 (MQ=255) tACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGCCAGTAAACTGACCCGCtcga < 2:473288/119‑4 (MQ=21) tACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGCCAGTAAACTGACCCGCtcga > 1:473288/1‑116 (MQ=21) gCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAatcat > 2:315184/1‑149 (MQ=255) tCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGc < 1:315184/149‑1 (MQ=255) aaTAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAatcat > 2:234701/1‑141 (MQ=255) aaTAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAatcat < 1:234701/141‑1 (MQ=255) gATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAg < 2:190452/149‑1 (MQ=255) agagATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTCATGCCAAATCATCAATGCAATCAGGCTGac > 4:219604/1‑149 (MQ=255) gCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGa < 2:17305/98‑1 (MQ=255) gCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGa > 1:17305/1‑98 (MQ=255) tGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAatcatc < 4:33857/116‑1 (MQ=255) tGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAatcatc > 3:33857/1‑116 (MQ=255) tGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACca > 3:52344/1‑146 (MQ=255) tGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACca < 4:52344/146‑1 (MQ=255) aGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAAAGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTgg > 2:53506/1‑149 (MQ=255) gCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGAc < 3:37313/149‑1 (MQ=255) tttGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCa > 1:9254/1‑149 (MQ=255) tttGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGC‑‑TTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCAcc > 3:344667/1‑149 (MQ=255) aaaaGCGGGCCAGTAAACTGACCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAAt < 2:414122/72‑1 (MQ=25) aaaaGCGGGCCAGTAAACTGACCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAAt > 1:414122/4‑75 (MQ=37) gAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAAAGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAgcggc < 1:53506/149‑1 (MQ=255) ggTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAAt < 2:188171/101‑1 (MQ=255) ggTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAAt > 1:188171/1‑101 (MQ=255) gTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATaa > 2:175848/1‑149 (MQ=255) cAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAAt > 1:8783/1‑149 (MQ=255) tttACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATtca > 4:463057/1‑149 (MQ=255) | GCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCA > NC_000913/3639196‑3639490 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |