Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,736,323 A→G 6.1% intergenic (+166/+30) xylR → / ← bax xylose divergent operon transcriptional activator/putative glucosaminidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,736,3230AG 6.1% 541.7 / 17.4 164intergenic (+166/+30)xylR/baxxylose divergent operon transcriptional activator/putative glucosaminidase
Reads supporting (aligned to +/- strand):  ref base A (79/75);  new base G (4/6);  total (83/81)
Fisher's exact test for biased strand distribution p-value = 5.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01

Only 100 of 169 total aligned reads displayed.

GGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGCTGTGGCAGT  >  NC_000913/3736175‑3736469
                                                                                                                                                    |                                                                                                                                                  
ggCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACGACGCTTCAGGCTCGTAGCTCAACTGCCTAAGACAAAGTAAAAAGCCTTATCCGACTGa                                                                                                                                                    <  2:198218/149‑1 (MQ=255)
ggCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGa                                                                                                                                                    >  4:222951/1‑149 (MQ=255)
ggCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGa                                                                                                                                                    <  3:400855/149‑1 (MQ=255)
      acaTCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTc                                                                                                                                              <  4:376930/149‑1 (MQ=255)
        aTCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCgg                                                                                                                                            >  3:439481/1‑149 (MQ=255)
           aGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATa                                                                                                                                         >  2:308717/1‑149 (MQ=255)
            ggTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATaa                                                                                                                                        <  2:220362/149‑1 (MQ=255)
             gTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCAGTCGGATAAg                                                                                                                                       <  1:156948/149‑1 (MQ=255)
                aCGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCt                                                                                                                                    >  3:417958/1‑149 (MQ=255)
                  gCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCa                                                                                                                                  >  4:269311/1‑149 (MQ=255)
                     gCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAgg                                                                                                                                      >  4:138651/1‑142 (MQ=255)
                     gCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAgg                                                                                                                                      <  3:138651/142‑1 (MQ=255)
                         ggTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGTACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAAt                                                                                                                           <  1:190871/149‑1 (MQ=255)
                              tCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAAt                                                                                                                      <  4:439481/149‑1 (MQ=255)
                                      ggcgTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATg                                                                                                              >  4:140655/1‑149 (MQ=255)
                                       gcgTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATgc                                                                                                             >  1:142053/1‑149 (MQ=255)
                                         gTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATgcgc                                                                                                           >  2:422254/1‑149 (MQ=255)
                                            aaCGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCAGTCGGATAAg                                                                                                                                       >  4:242447/1‑118 (MQ=39)
                                            aaCGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCAGTCGGATAAg                                                                                                                                       <  3:242447/118‑1 (MQ=39)
                                            aaCGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTc                                                                                                                                              >  2:137425/1‑111 (MQ=255)
                                            aaCGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTc                                                                                                                                              <  1:137425/111‑1 (MQ=255)
                                               gCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCAGTCGGATaa                                                                                                                                        >  4:350477/1‑114 (MQ=39)
                                               gCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGAt                                                                                                     >  3:158411/1‑149 (MQ=255)
                                               gCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGAt                                                                                                     <  4:417958/149‑1 (MQ=255)
                                                 cTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGa                                                                                                      <  2:371328/146‑1 (MQ=255)
                                                 cTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGa                                                                                                      >  1:371328/1‑146 (MQ=255)
                                                 cTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGAtt                                                                                                    >  4:63631/1‑148 (MQ=255)
                                                 cTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGAtt                                                                                                    <  3:63631/148‑1 (MQ=255)
                                                 cTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTa                                                                                                   >  2:205005/1‑149 (MQ=255)
                                                 cTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTa                                                                                                   >  1:473459/1‑149 (MQ=255)
                                                   tATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAAc                                                                                                 <  2:63383/149‑1 (MQ=255)
                                                    aTCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAg                                                                                                                                >  2:142574/1‑117 (MQ=255)
                                                    aTCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAg                                                                                                                                <  1:142574/117‑1 (MQ=255)
                                                    aTCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAAcc                                                                                                >  2:121944/1‑149 (MQ=255)
                                                     tCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCg                                                                                               >  2:188357/1‑149 (MQ=255)
                                                     tCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCg                                                                                               >  4:262831/1‑149 (MQ=255)
                                                      ccGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGt                                                                                              <  4:158411/149‑1 (MQ=255)
                                                      ccGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGt                                                                                              <  2:69703/149‑1 (MQ=255)
                                                        gACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTCGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTg                                                                                            >  2:141904/1‑149 (MQ=255)
                                                        gACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTg                                                                                            <  3:269311/149‑1 (MQ=255)
                                                         aCCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAg                                                                                                                                       <  1:348306/105‑1 (MQ=255)
                                                         aCCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAg                                                                                                                                       >  2:348306/1‑105 (MQ=255)
                                                              cccGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATc                                                                                      <  1:391618/149‑1 (MQ=255)
                                                               ccGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATcc                                                                                     >  3:468378/1‑149 (MQ=255)
                                                                 gCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTg                                                                                   >  2:385888/1‑149 (MQ=255)
                                                                    aGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTa                                                                                >  2:241180/1‑149 (MQ=255)
                                                                          taTAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTTGTACATTGc                                                                          <  4:306345/148‑1 (MQ=255)
                                                                          gATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGc                                                                          >  3:367091/1‑149 (MQ=255)
                                                                           aTAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTc                                                                                                                                              >  2:383144/1‑80 (MQ=255)
                                                                           aTAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTc                                                                                                                                              <  1:383144/80‑1 (MQ=255)
                                                                           aTAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCg                                                                         >  3:70650/1‑149 (MQ=255)
                                                                           aTAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCg                                                                         >  2:246568/1‑149 (MQ=255)
                                                                             aaaaCGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGaa                                                                       <  4:468378/149‑1 (MQ=255)
                                                                             aaaaCGCACCAACAACGCCTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTg                                                                                            <  1:164536/128‑1 (MQ=255)
                                                                             aaaaCGCACCAACAACGCCTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTg                                                                                            >  2:164536/1‑128 (MQ=255)
                                                                               aaCGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACa                                                                     <  1:205005/149‑1 (MQ=255)
                                                                               aaCGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACa                                                                     >  3:354964/1‑149 (MQ=255)
                                                                               aaCGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACa                                                                     >  1:23093/1‑149 (MQ=255)
                                                                                aCGCACCAACAACGCTTCAGGCTCGTAGCTGAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAg                                                                    <  1:308717/149‑1 (MQ=255)
                                                                                aCGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAg                                                                    <  4:367091/149‑1 (MQ=255)
                                                                                  gCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAgg                                                                                                                                      >  3:25180/1‑81 (MQ=255)
                                                                                  gCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAgg                                                                                                                                      <  4:25180/81‑1 (MQ=255)
                                                                                      caacaaCGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAgtt                                                              <  1:177359/149‑1 (MQ=255)
                                                                                       aacaacGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATc                                                                                      <  2:450362/124‑1 (MQ=255)
                                                                                       aacaacGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATc                                                                                      >  1:450362/1‑124 (MQ=255)
                                                                                        gcaacGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAgttgt                                                            >  2:404117/2‑149 (MQ=255)
                                                                                         caacGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAGAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTAc                                                                               >  3:310798/1‑129 (MQ=255)
                                                                                         caacGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTAc                                                                               <  4:310798/129‑1 (MQ=255)
                                                                                          aacGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCTGATAAGGCt                                                                                                                                    >  1:38707/1‑75 (MQ=255)
                                                                                                  aGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTGGTTATAACTCtt                                                  >  3:198316/1‑149 (MQ=255)
                                                                                                    gCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTcc                                                <  3:262831/149‑1 (MQ=255)
                                                                                                        gTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTg                                                                                   >  3:73370/1‑110 (MQ=255)
                                                                                                         tAGCTCAACTGCCTGAGACAAAGTAACAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTg                                           >  4:88253/1‑149 (MQ=255)
                                                                                                         tAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGCACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTg                                           <  4:392902/149‑1 (MQ=255)
                                                                                                             tCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCtt                                             <  1:326880/143‑1 (MQ=255)
                                                                                                               aaCTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTc                                        >  4:178389/1‑146 (MQ=255)
                                                                                                                    ccTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGcc                                <  1:110182/149‑1 (MQ=255)
                                                                                                                     cTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGccc                               <  4:196428/149‑1 (MQ=255)
                                                                                                                      tGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCt                              >  3:391973/1‑149 (MQ=255)
                                                                                                                         gaCAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCa                           >  1:111717/1‑149 (MQ=255)
                                                                                                                            aaaGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTc                                        <  3:307441/133‑1 (MQ=255)
                                                                                                                            aaaGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCa                           >  3:196539/1‑146 (MQ=255)
                                                                                                                            aaaGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCAATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTc                                        >  4:307441/1‑133 (MQ=255)
                                                                                                                              aGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCCGGTACAtt                                                                            <  3:334478/95‑1 (MQ=255)
                                                                                                                               gTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTtgt                     <  2:473459/149‑1 (MQ=255)
                                                                                                                               gTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTtgt                     >  4:344089/1‑149 (MQ=255)
                                                                                                                                  aaaaGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCtt                       <  1:220930/144‑1 (MQ=255)
                                                                                                                                       ccTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAatcatc             <  2:341646/149‑1 (MQ=255)
                                                                                                                                        cTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGt                                                                                 <  4:23636/80‑1 (MQ=255)
                                                                                                                                        cTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGt                                                                                 >  3:23636/1‑80 (MQ=255)
                                                                                                                                         ttATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGc           >  2:185874/1‑149 (MQ=255)
                                                                                                                                            tCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGCtgt        >  3:145207/1‑149 (MQ=255)
                                                                                                                                            tCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGCtgt        <  1:51502/149‑1 (MQ=255)
                                                                                                                                            tCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGCtgt        >  3:170646/1‑149 (MQ=255)
                                                                                                                                            tCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGCtgt        <  1:246568/149‑1 (MQ=255)
                                                                                                                                              cGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGCTGTgg      >  2:439607/1‑149 (MQ=255)
                                                                                                                                               gACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTgg                                                                                  <  2:227084/72‑1 (MQ=255)
                                                                                                                                                aCTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGCTGTGGCa    <  4:198316/149‑1 (MQ=255)
                                                                                                                                                  tGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTg                                           >  1:253253/1‑108 (MQ=255)
                                                                                                                                                  tGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGCTGTGGCAGt  <  4:70650/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGCTGTGGCAGT  >  NC_000913/3736175‑3736469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: