Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,938,255 T→A 8.2% L10Q (CTG→CAG)  rbsR → transcriptional repressor of ribose metabolism

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,938,2550TA 8.2% 164.9 / 6.1 49L10Q (CTG→CAG) rbsRtranscriptional repressor of ribose metabolism
Reads supporting (aligned to +/- strand):  ref base T (20/25);  new base A (2/2);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.09e-01

CGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAA  >  NC_000913/3938114‑3938400
                                                                                                                                             |                                                                                                                                                 
cGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCAggcgggc                                                                                                                                            <  1:91214/149‑1 (MQ=255)
  aTTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGt                                                                                                                                          >  1:160018/1‑149 (MQ=255)
  aTTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGt                                                                                                                                          <  1:315509/149‑1 (MQ=255)
  aTTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGt                                                                                                                                          <  3:27164/149‑1 (MQ=255)
   ttCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGtt                                                                                                                                         <  1:349690/149‑1 (MQ=255)
    tCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGttt                                                                                                                                        <  2:290489/149‑1 (MQ=255)
     cGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTc                                                                                                                                       <  4:135915/149‑1 (MQ=255)
       ttttGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTa                                                                                                                                     >  4:171637/1‑149 (MQ=255)
              cATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAAc                                                                                                                              <  4:373655/149‑1 (MQ=255)
               aTGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGttt                                                                                                                                        >  2:134844/1‑138 (MQ=255)
               aTGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGttt                                                                                                                                        <  1:134844/138‑1 (MQ=255)
                             aTTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTAt                                                                                                               <  2:65945/149‑1 (MQ=255)
                             aTTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTAt                                                                                                               >  4:343446/1‑149 (MQ=255)
                              ttGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATc                                                                                                              <  1:472627/149‑1 (MQ=255)
                                 ccGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAAt                                                                                                           <  2:160018/149‑1 (MQ=255)
                                    tAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATaaa                                                                                                        <  1:239491/149‑1 (MQ=255)
                                        acGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATc                                                                                                    <  3:171637/149‑1 (MQ=255)
                                        acGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATc                                                                                                    <  1:230153/149‑1 (MQ=255)
                                        acGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATc                                                                                                    >  4:162882/1‑149 (MQ=255)
                                           tAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCt                                                                                                 >  3:446661/1‑149 (MQ=255)
                                             aaGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTc                                                                                               <  1:447301/149‑1 (MQ=255)
                                                  gcACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTggcgg                                                                                                                                              <  3:242895/97‑1 (MQ=255)
                                                  gcACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTggcgg                                                                                                                                              >  4:242895/1‑97 (MQ=255)
                                                  gcACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTATAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAg                                                                                          <  1:388373/149‑1 (MQ=255)
                                                    acaACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTg                                                                                        >  2:4720/1‑149 (MQ=255)
                                                         cTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATaaa                                                                                                        >  1:324714/1‑128 (MQ=255)
                                                         cTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCGCACGTTATCAATaaa                                                                                                        <  2:324714/128‑1 (MQ=255)
                                                              gTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTAc                                                                              >  4:218165/1‑149 (MQ=255)
                                                                            agagATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAg                                                                <  3:343446/149‑1 (MQ=255)
                                                                                aTCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGAtt                                                                                >  4:118493/1‑129 (MQ=255)
                                                                                aTCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGAtt                                                                                <  3:118493/129‑1 (MQ=255)
                                                                                aTCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAgcggc                                                            <  3:162882/149‑1 (MQ=255)
                                                                                 tCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAgcggcg                                                           >  3:204480/1‑149 (MQ=255)
                                                                                       cATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTaaa                                                     <  3:434713/149‑1 (MQ=255)
                                                                                                            gACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCa                                >  3:164748/1‑149 (MQ=255)
                                                                                                               gCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGct                             >  2:5373/1‑149 (MQ=255)
                                                                                                                         aaTGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTa                   >  2:386791/1‑149 (MQ=255)
                                                                                                                            gAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTaaa                                                     >  1:226511/1‑112 (MQ=255)
                                                                                                                            gAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTaaa                                                     <  2:226511/112‑1 (MQ=255)
                                                                                                                               aGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCa             >  3:194817/1‑149 (MQ=255)
                                                                                                                                gATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCaa            >  4:74551/1‑149 (MQ=255)
                                                                                                                                 gaaacgCCCGCCAGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGctct                           >  4:27455/6‑133 (MQ=255)
                                                                                                                                 gaaacgCCCGCCAGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGctct                           <  3:27455/128‑1 (MQ=255)
                                                                                                                                 aTGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCaaa           <  4:204480/149‑1 (MQ=255)
                                                                                                                                 aTGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCaaa           <  1:5373/149‑1 (MQ=255)
                                                                                                                                 aTGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCaaa           <  3:74551/149‑1 (MQ=255)
                                                                                                                                   aacgCCCGCCAGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACt         >  1:248422/4‑149 (MQ=255)
                                                                                                                                      gCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACtcaa      >  4:178434/1‑149 (MQ=255)
                                                                                                                                          gCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACtcaatcaa  >  4:412768/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
CGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAA  >  NC_000913/3938114‑3938400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑