| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,959,924 | A→T | 11.1% | intergenic (‑111/+88) | ppiC ← / ← yifN | peptidyl‑prolyl cis‑trans isomerase C (rotamase C)/PemK toxin family pseudogene |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,959,924 | 0 | A | T | 11.1% | 111.2 / 5.7 | 36 | intergenic (‑111/+88) | ppiC/yifN | peptidyl‑prolyl cis‑trans isomerase C (rotamase C)/PemK toxin family pseudogene |
| Reads supporting (aligned to +/- strand): ref base A (14/18); new base T (2/2); total (16/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01 | |||||||||||
AGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGT > NC_000913/3959782‑3960064 | aGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCg < 1:380113/149‑1 (MQ=255)aGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTCCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCg < 4:36924/149‑1 (MQ=255) gCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGATTTTCCGGGCtttt < 1:68986/149‑3 (MQ=255) cAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGc > 4:343513/1‑149 (MQ=255) ctgctgTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCt > 1:467324/1‑149 (MQ=255) gCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGaa < 3:441708/149‑1 (MQ=255) aTCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGTAAAAGCCCGGAACATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTa > 1:85989/1‑149 (MQ=255) tCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAg < 3:343513/149‑1 (MQ=255) tGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATggg > 1:342548/1‑149 (MQ=255) aatTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACa > 4:134789/1‑149 (MQ=255) ttGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaaa > 4:274131/1‑149 (MQ=255) gcggcgATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTa > 2:161470/1‑149 (MQ=255) ggcgATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATc < 1:161470/149‑1 (MQ=255) aTTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTGTCgggg < 3:8267/149‑1 (MQ=255) ttttAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGa < 2:370109/149‑1 (MQ=255) tGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt > 3:69963/1‑149 (MQ=255) cGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttatt < 4:69963/149‑1 (MQ=255) aGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTc < 2:467324/149‑1 (MQ=255) agcGTAATTGTCAGTCAGGGAGTGGCTGGTCCCTGTGTGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTGAGGGCCTCTATGGGCTACAAAGTCATTATCGGTTATGGTTATTTTATTGTCGAGTTAt < 2:85989/147‑1 (MQ=255) gTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAg > 4:135339/1‑149 (MQ=255) aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGa > 2:390207/1‑149 (MQ=255) aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGa > 2:185989/1‑149 (MQ=255) tcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGATTTTCCGGGCtttt > 4:436084/1‑50 (MQ=21) tcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGATTTTCCGGGCtttt < 3:436084/52‑3 (MQ=25) gAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCattgatt < 1:185989/149‑1 (MQ=255) gCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACtt < 3:134789/149‑1 (MQ=255) tGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTtga < 2:334443/149‑1 (MQ=255) ggTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTtgat < 3:135339/149‑1 (MQ=255) tatGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGccc < 2:342548/149‑1 (MQ=255) cAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTtga > 3:296231/1‑135 (MQ=255) cAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTtga < 4:296231/135‑1 (MQ=255) aGCCCGGATTTTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGt > 2:34227/1‑149 (MQ=255) cccGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTa > 3:260755/1‑144 (MQ=255) cccGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTa < 4:260755/144‑1 (MQ=255) cGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGccc > 3:426997/1‑136 (MQ=255) cGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGccc < 4:426997/136‑1 (MQ=255) | AGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGT > NC_000913/3959782‑3960064 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |