Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,982,923 A→G 9.1% intergenic (+112/‑35) proM → / → aslB tRNA‑Pro/putative AslA‑specific sulfatase‑maturating enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,982,9230AG 9.1% 130.5 / 4.2 44intergenic (+112/‑35)proM/aslBtRNA‑Pro/putative AslA‑specific sulfatase‑maturating enzyme
Reads supporting (aligned to +/- strand):  ref base A (23/17);  new base G (2/2);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

GGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGC  >  NC_000913/3982784‑3983071
                                                                                                                                           |                                                                                                                                                    
ggAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGaa                                                                                                                                             >  3:295802/1‑149 (MQ=255)
                    gCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGa                                                                                                                                          <  1:48630/132‑1 (MQ=255)
                    gCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGa                                                                                                                                          >  2:48630/1‑132 (MQ=255)
                       gACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGa                                                                                                                      <  3:430456/149‑1 (MQ=255)
                                                  ggTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCa                                                                                                                   >  1:413543/1‑125 (MQ=255)
                                                  ggTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCa                                                                                                                   <  2:413543/125‑1 (MQ=255)
                                                       tttGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCATTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCtttt                                                                                      <  4:295802/149‑1 (MQ=255)
                                                           ttttCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAAc                                                                                                                                                     >  3:418924/1‑82 (MQ=255)
                                                           ttttCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAAc                                                                                                                                                     <  4:418924/82‑1 (MQ=255)
                                                               cTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACATCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGAt                                                                              <  2:408960/149‑1 (MQ=255)
                                                                tgtgCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATg                                                                             >  3:78265/1‑149 (MQ=255)
                                                                             aCCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCg                                                                >  4:198052/1‑149 (MQ=255)
                                                                                 tccctTCGCAATAAACGCCCGTAATAACTCATTGCCCCATGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTc                                                            >  4:140004/1‑149 (MQ=255)
                                                                                 tccctTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTc                                                            <  4:9165/149‑1 (MQ=255)
                                                                                 tccctTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTc                                                            >  1:198754/1‑149 (MQ=255)
                                                                                           aTAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAAt                                                  <  3:22755/149‑1 (MQ=255)
                                                                                                    cGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACtgt                                         >  2:13371/1‑149 (MQ=255)
                                                                                                      taataaCTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGa                                       >  1:344214/1‑149 (MQ=255)
                                                                                                           aCTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGactact                                  >  2:425531/1‑149 (MQ=255)
                                                                                                             tCATTGCCCCACGGTATGATTTCGCCCTTAGCGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGt                                >  4:147374/1‑149 (MQ=255)
                                                                                                             tCATTGCCCCACGGTATGATTTCGCCCTTAGCGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGt                                >  2:401834/1‑149 (MQ=255)
                                                                                                             tCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGAtt                                                       <  1:131507/126‑1 (MQ=255)
                                                                                                             tCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGAtt                                                       >  2:131507/1‑126 (MQ=255)
                                                                                                             tCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGt                                >  3:3363/1‑149 (MQ=255)
                                                                                                              cATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGtt                               >  2:205202/1‑149 (MQ=255)
                                                                                                               aTTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCAtgt                                                                                 <  2:305095/98‑1 (MQ=255)
                                                                                                               aTTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCAtgt                                                                                 >  1:305095/1‑98 (MQ=255)
                                                                                                                ttGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGc                                                                                          >  3:267404/1‑88 (MQ=255)
                                                                                                                ttGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGc                                                                                          <  4:267404/88‑1 (MQ=255)
                                                                                                                ttGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGtttt                             <  2:344214/149‑1 (MQ=255)
                                                                                                                   ccccACGGTATGATTTCGCCCTTAGCGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTAt                          <  1:401834/149‑1 (MQ=255)
                                                                                                                   ccccACGGTATGATTTCGCCCTTAGCGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTAt                          <  3:147374/149‑1 (MQ=255)
                                                                                                                    cccACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTAtc                         >  1:51721/1‑149 (MQ=255)
                                                                                                                      cACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTAtctc                       <  4:3363/149‑1 (MQ=255)
                                                                                                                           tATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCTACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGCTTGTAATCTGAACTGTGACTACTGTGTTTATCTCGaaaa                  >  3:390015/1‑149 (MQ=255)
                                                                                                                           tATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGaaaa                  >  3:221935/1‑149 (MQ=255)
                                                                                                                           tATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGaaaa                  >  1:344750/1‑149 (MQ=255)
                                                                                                                              gATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACa               >  2:256562/1‑149 (MQ=255)
                                                                                                                               aTTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACaa              >  4:8409/1‑149 (MQ=255)
                                                                                                                               aTTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACaa              <  4:78265/149‑1 (MQ=255)
                                                                                                                               aTTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACaa              >  2:472366/1‑149 (MQ=255)
                                                                                                                                tttCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAAt             <  3:198052/149‑1 (MQ=255)
                                                                                                                                       cTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTa      <  1:425531/149‑1 (MQ=255)
                                                                                                                                        ttAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTAc     <  1:13371/149‑1 (MQ=255)
                                                                                                                                           aCGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACcgc  >  2:369210/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                                    
GGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGC  >  NC_000913/3982784‑3983071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑