Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,060,264 A→C 22.8% intergenic (+34/‑183) typA → / → yihL GTP‑binding protein/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,060,2640AC22.8% 83.3 / 30.7 44intergenic (+34/‑183)typA/yihLGTP‑binding protein/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (15/19);  new base C (4/6);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-02

ATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCTTTTTTGGCGTAA  >  NC_000913/4060120‑4060404
                                                                                                                                                |                                                                                                                                             
aTCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGc                                                                                                                                           <  1:177219/149‑1 (MQ=255)
 tCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCa                                                                                                                                          <  4:216340/149‑1 (MQ=255)
 tCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCa                                                                                                                                          >  3:226458/1‑149 (MQ=255)
   gatgatGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAgg                                                                                                                                        >  2:394842/1‑149 (MQ=255)
     tgatgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGG‑tt                                                                                                                                     <  2:31032/149‑1 (MQ=255)
      gatgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGG‑ttt                                                                                                                                    <  2:203761/149‑1 (MQ=255)
             aaCTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTg                                                                                                                             >  3:53438/1‑149 (MQ=255)
                  gTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGt                                                                                                                        >  2:169862/1‑149 (MQ=255)
                    aGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTg                                                                                                                             <  3:205112/142‑1 (MQ=255)
                    aGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTg                                                                                                                             >  4:205112/1‑142 (MQ=255)
                      aaGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGG‑ttttttatga                                                                                                                             >  1:327396/1‑136 (MQ=255)
                      aaGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGG‑ttttttatga                                                                                                                             <  2:327396/140‑5 (MQ=255)
                             tCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑ttttttttt                                                                                                                              <  2:81876/132‑1 (MQ=255)
                             tCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGcac                                                                                                             >  4:119424/1‑149 (MQ=255)
                             tCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAATCATAAAAAACCTGCCAGCGATGGCAGG‑ttttttttt                                                                                                                              >  1:81876/1‑132 (MQ=255)
                             tCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTCTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTg                                                                                                                    >  2:340909/1‑142 (MQ=255)
                             tCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCACGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTg                                                                                                                    <  1:340909/142‑1 (MQ=255)
                                          cGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAGACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGa                                                                                                   <  4:38410/146‑1 (MQ=255)
                                          cGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGa                                                                                                   >  3:38410/1‑146 (MQ=255)
                                           gTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAt                                                                                               >  2:78927/1‑149 (MQ=255)
                                                    aaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtat                                                                                      <  3:119424/149‑1 (MQ=255)
                                                        tCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGt                                                                                  <  4:3445/149‑1 (MQ=255)
                                                           ccTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCt                                                                                <  2:254750/149‑1 (MQ=255)
                                                              gACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTgg                                                                            <  1:169862/149‑1 (MQ=255)
                                                               aCGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGTCGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGt                                                                           <  2:367123/149‑1 (MQ=255)
                                                                     aaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGG‑ttt                                                                                                                                    >  1:160549/1‑86 (MQ=255)
                                                                     aaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGG‑ttt                                                                                                                                    <  2:160549/86‑1 (MQ=255)
                                                                           cgtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATaga                                                               <  4:53438/149‑1 (MQ=255)
                                                                           cgtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATaga                                                               <  4:226458/149‑1 (MQ=255)
                                                                                       aaCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTcac                                                   >  4:150653/1‑149 (MQ=255)
                                                                                                    aaGACGATTAATTTCGTTTTTTAGTCATAAAAATCCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCaaa                                      <  1:78927/149‑1 (MQ=255)
                                                                                                       aCGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTc                                   <  3:32418/149‑1 (MQ=255)
                                                                                                         gATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTAAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCAt                                 <  3:368424/149‑1 (MQ=255)
                                                                                                               tttCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCg                           <  4:183130/149‑1 (MQ=255)
                                                                                                                    ttttttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATa                                                                 >  2:396201/1‑106 (MQ=255)
                                                                                                                    ttttttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATa                                                                 <  1:396201/106‑1 (MQ=255)
                                                                                                                       tttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGc                   <  3:150653/149‑1 (MQ=255)
                                                                                                                                aaaaaaCCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCttttt          >  1:208931/1‑149 (MQ=255)
                                                                                                                                aaaaaaCCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAACTCATACGCCGTTAATTGCTTTCttttt          <  2:208931/149‑1 (MQ=255)
                                                                                                                                 aaaaaCCTGCCATCGCTGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACaa                                                 >  1:122843/1‑109 (MQ=255)
                                                                                                                                 aaaaaCCTGCCATCGCTGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACaa                                                 <  2:122843/109‑1 (MQ=255)
                                                                                                                                    aaCCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCTTTTTTGgc      >  1:158623/1‑149 (MQ=255)
                                                                                                                                      ccTGCCATCGCTGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCTTTTTTGgcgt    >  3:429396/1‑149 (MQ=255)
                                                                                                                                        tGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCTTTTTTGgcgtaa  >  2:433576/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                             
ATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCTTTTTTGGCGTAA  >  NC_000913/4060120‑4060404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑