Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,114,536 C→T 7.5% intergenic (‑64/+33) fpr ← / ← glpX ferredoxin‑NADP reductase; flavodoxin reductase/fructose 1,6‑bisphosphatase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,114,5360CT 7.5% 208.3 / 5.0 66intergenic (‑64/+33)fpr/glpXferredoxin‑NADP reductase; flavodoxin reductase/fructose 1,6‑bisphosphatase II
Reads supporting (aligned to +/- strand):  ref base C (29/32);  new base T (2/3);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGAT  >  NC_000913/4114388‑4114682
                                                                                                                                                    |                                                                                                                                                  
gcgcGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTccc                                                                                                                                                    >  3:282081/4‑149 (MQ=255)
  aCGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGa                                                                                                                                                  >  2:353516/1‑149 (MQ=255)
    gggggCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAg                                                                                                                                                <  3:140063/149‑1 (MQ=255)
     ggggCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAgg                                                                                                                                               <  4:241985/149‑1 (MQ=255)
            gAACGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCTTCGGGACTGGaa                                                                                                                                        <  1:331329/149‑1 (MQ=255)
             aaCGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAg                                                                                                                                       <  1:137841/149‑1 (MQ=255)
                ggTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCt                                                                                                                                    <  3:400467/149‑1 (MQ=255)
                        tAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGat                                                                                                                            <  2:99462/149‑1 (MQ=255)
                          aaCAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCTTCGGGACTGGaat                                                                                                                                       <  1:30555/136‑2 (MQ=255)
                          aaCAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCTTCGGGACTGGaat                                                                                                                                       >  2:30555/1‑135 (MQ=255)
                          aaCAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGatca                                                                                                                          >  2:58721/1‑149 (MQ=255)
                          aaCAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGat                                                                                                                            >  2:293999/1‑147 (MQ=255)
                          aaCAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGat                                                                                                                            <  1:293999/147‑1 (MQ=255)
                              gggCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGatcaaatc                                                                                                                      <  4:444695/149‑1 (MQ=255)
                                            ttCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGAtgtg                                                                                                        <  4:282081/149‑1 (MQ=255)
                                            ttCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGAtgtg                                                                                                        <  1:52911/149‑1 (MQ=255)
                                                  aCTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTg                                                                                                  >  2:263455/1‑149 (MQ=255)
                                                  aCTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTg                                                                                                  >  1:210480/1‑149 (MQ=255)
                                                        gTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAatcaatca                                                                                                                 <  2:61745/128‑1 (MQ=255)
                                                        gTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAatcaatca                                                                                                                 >  1:61745/1‑128 (MQ=255)
                                                         tGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTcc                                                                                           >  2:175245/1‑149 (MQ=255)
                                                             tttGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAaatcaat                                                                                                                   >  4:251829/1‑121 (MQ=255)
                                                             tttGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAaatcaat                                                                                                                   <  3:251829/121‑1 (MQ=255)
                                                             tttGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGt                                                                                       <  1:263455/149‑1 (MQ=255)
                                                             tttGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTAATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGt                                                                                       >  1:402908/1‑149 (MQ=255)
                                                             tttGCCTGTTACCCAATCAGCCATGTTTGTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGt                                                                                       >  3:31822/1‑149 (MQ=255)
                                                                   tGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGc                                                                                 <  1:68908/149‑1 (MQ=255)
                                                                    gTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCgg                                                                                         >  1:162565/1‑140 (MQ=255)
                                                                    gTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCgg                                                                                         <  2:162565/140‑1 (MQ=255)
                                                                     ttACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCAt                                                                                               <  1:292290/133‑1 (MQ=255)
                                                                     ttACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCAt                                                                                               >  2:292290/1‑133 (MQ=255)
                                                                         ccAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATcc                                                                            >  3:30622/1‑148 (MQ=255)
                                                                         ccAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATcc                                                                            <  4:30622/148‑1 (MQ=255)
                                                                           aaTCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAatcaatca                                                                                                                 >  4:128157/1‑109 (MQ=255)
                                                                           aaTCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAatcaatca                                                                                                                 <  3:128157/109‑1 (MQ=255)
                                                                           aaTCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGa                                                                         <  3:120686/149‑1 (MQ=255)
                                                                                gCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGtg                                                                    <  1:175245/149‑1 (MQ=255)
                                                                                    tGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCaga                                                                                                               <  1:94062/102‑1 (MQ=255)
                                                                                    tGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCaga                                                                                                               >  2:94062/1‑102 (MQ=255)
                                                                                    tGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGtg                                                                    <  3:337549/145‑1 (MQ=255)
                                                                                    tGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGtg                                                                    >  4:337549/1‑145 (MQ=255)
                                                                                       ttttCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGAc                                                             <  2:210480/149‑1 (MQ=255)
                                                                                                gTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATgcgg                                                    >  4:271909/1‑149 (MQ=255)
                                                                                                 ttttGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATgcggc                                                   >  2:358206/1‑149 (MQ=255)
                                                                                                      attgaCCTGACTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCCGCATTTCCGGGTCTTTGCGATCCAGCTAGTGGATGGACTGAATGCGGCGAATg                                              <  2:402908/149‑1 (MQ=255)
                                                                                                        tgaCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGt                                            <  1:58721/149‑1 (MQ=255)
                                                                                                            ttGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGt                                        >  2:159230/1‑149 (MQ=255)
                                                                                                                 ttATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATg                                                       >  2:114705/1‑129 (MQ=255)
                                                                                                                 ttATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATg                                                       <  1:114705/129‑1 (MQ=255)
                                                                                                                 ttATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACtt                                   >  4:439870/1‑149 (MQ=255)
                                                                                                                     cTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAAt                                                        <  3:210792/124‑1 (MQ=255)
                                                                                                                     cTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAAt                                                        >  4:210792/1‑124 (MQ=255)
                                                                                                                         gTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGa                           <  1:202999/149‑1 (MQ=255)
                                                                                                                         gTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGa                           >  1:377779/1‑149 (MQ=255)
                                                                                                                            ctcCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATca                        <  3:439870/149‑1 (MQ=255)
                                                                                                                             tcCGAACAAAAAAATGCCAGTCCCGAAGGAGTGGAGGGCTCAATCGATCAAATCAATCAGAGGATGTGCGCCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGTATGGTGCGTGACTTGCCGCGGATcag                       >  4:295113/1‑149 (MQ=255)
                                                                                                                              ccGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcagc                      >  2:201069/1‑149 (MQ=255)
                                                                                                                                  aCAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCg                  >  4:70068/1‑149 (MQ=255)
                                                                                                                                    aaaaaaaTTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCgg                            >  2:401385/1‑137 (MQ=255)
                                                                                                                                    aaaaaaaTTCCAGTCCCGAAGGACTGGAAGGCTCAAACGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCgg                            <  1:401385/137‑1 (MQ=255)
                                                                                                                                       aaaaTTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCGGCAGCGTTTCg             <  1:353516/149‑1 (MQ=255)
                                                                                                                                          cttCCAGTCCTTCGGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGtgga                                                                  <  4:322774/92‑1 (MQ=255)
                                                                                                                                           ttCCAGTCCTTCGGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAg         >  4:144671/1‑149 (MQ=255)
                                                                                                                                              cAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGGGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTcg      >  4:172462/1‑149 (MQ=255)
                                                                                                                                              cAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGa                                      >  4:239744/1‑117 (MQ=255)
                                                                                                                                              cAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGa                                      <  3:239744/117‑1 (MQ=255)
                                                                                                                                                 tCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGa   <  2:377779/149‑1 (MQ=255)
                                                                                                                                                  cccGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGat  <  1:358206/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGAT  >  NC_000913/4114388‑4114682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: