Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,117,057	T→C	100%	T56A (ACC→GCC)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,117,057	0	T	C	100.0%	145.8 / NA	42	T56A (ACC→GCC)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/22);  total (20/22)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCG  >  NC_000913/4116921‑4117193                                                                                                                                         |                                                                                                                                         gCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTc                                                                                                                              <  3:191731/149‑1 (MQ=255)    ttATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCAt                                                                                                                           >  3:305568/1‑149 (MQ=255)    ttATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCAt                                                                                                                           >  3:173584/1‑149 (MQ=255)        agatagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCTTGGAGCTTTGGGCGGCCCAGATTTCCATTggg                                                                                                                       >  4:289435/1‑149 (MQ=255)                    cGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTAcc                                                                                                           >  3:330841/1‑149 (MQ=255)                       tttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCaa                                                                                                        >  4:244839/1‑149 (MQ=255)                       tttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCaa                                                                                                        >  2:394345/1‑149 (MQ=255)                        ttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCAtt                                                                                                                          >  4:471407/1‑130 (MQ=255)                        ttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCAtt                                                                                                                          <  3:471407/130‑1 (MQ=255)                         tCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAcc                                                                                                      <  3:246810/149‑1 (MQ=255)                           tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCa                                                                                                                                    >  4:166675/1‑117 (MQ=255)                           tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCa                                                                                                                                    <  3:166675/117‑1 (MQ=255)                           tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTg                                                                                                    >  2:455900/1‑149 (MQ=255)                                cccAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtttt                                                                                               <  3:340981/149‑1 (MQ=255)                                 ccAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTg                                                                                              <  1:292288/149‑1 (MQ=255)                                  cAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                                                             <  4:305568/149‑1 (MQ=255)                                         tAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAtt                                                                                      >  4:48677/1‑149 (MQ=255)                                              gTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTc                                                                                 <  1:455900/149‑1 (MQ=255)                                               tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCa                                                                                >  2:315812/1‑149 (MQ=255)                                                       tGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGCTTTGGGTAGATTTGCTCAAATTcgcg                                                                        >  2:148084/1‑149 (MQ=255)                                                           tCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATAGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGc                                                                    <  1:446842/149‑1 (MQ=255)                                                              tAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGc                                                                                   >  3:15991/1‑131 (MQ=255)                                                              tAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGc                                                                                   <  4:15991/131‑1 (MQ=255)                                                                     gATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa                                                          <  3:48677/149‑1 (MQ=255)                                                                     gATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGATaa                                                          <  1:394345/149‑1 (MQ=255)                                                                           tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAGCCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGatat                                                    >  1:400125/1‑149 (MQ=255)                                                                           tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGatat                                                    >  2:393616/1‑149 (MQ=255)                                                                                     tCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAAt                                                         <  2:308729/134‑1 (MQ=255)                                                                                     tCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTAGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCTCTGCGACACGCTAAt                                                         >  1:308729/1‑134 (MQ=255)                                                                                       ggAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGAt                                        <  3:244839/149‑1 (MQ=255)                                                                                       ggAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGAt                                        <  4:147392/149‑1 (MQ=255)                                                                                             gATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTa                                  <  1:393616/149‑1 (MQ=255)                                                                                                atCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCCTTGGGCGGCCCAGATTCCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGa                               <  2:400125/149‑1 (MQ=255)                                                                                                 tCGGCTTTCGCGAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTAGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGACATTGGCATCGTGATCCATTACGAc                              >  3:314033/1‑149 (MQ=255)                                                                                                                aCTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggt               <  1:148084/149‑1 (MQ=255)                                                                                                                 cTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg              <  4:197266/149‑1 (MQ=255)                                                                                                                  ttCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg             >  4:258743/1‑149 (MQ=255)                                                                                                                      aCCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGcc         <  2:249064/149‑1 (MQ=255)                                                                                                                           cGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGt    <  4:330841/149‑1 (MQ=255)                                                                                                                             tGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCg  >  1:457757/1‑149 (MQ=255)                                                                                                                               gAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCAtt                                   <  4:105747/114‑1 (MQ=255)                                                                                                                               gAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCAtt                                   >  3:105747/1‑114 (MQ=255)                                                                                                                                         |                                                                                                                                         GCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCG  >  NC_000913/4116921‑4117193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑