Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,117,057 T→C 100% T56A (ACC→GCC)  glpK ← glycerol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,117,0570TC100.0% 145.8 / NA 42T56A (ACC→GCC) glpKglycerol kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/22);  total (20/22)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCG  >  NC_000913/4116921‑4117193
                                                                                                                                        |                                                                                                                                        
gCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTc                                                                                                                              <  3:191731/149‑1 (MQ=255)
   ttATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCAt                                                                                                                           >  3:305568/1‑149 (MQ=255)
   ttATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCAt                                                                                                                           >  3:173584/1‑149 (MQ=255)
       agatagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCTTGGAGCTTTGGGCGGCCCAGATTTCCATTggg                                                                                                                       >  4:289435/1‑149 (MQ=255)
                   cGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTAcc                                                                                                           >  3:330841/1‑149 (MQ=255)
                      tttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCaa                                                                                                        >  4:244839/1‑149 (MQ=255)
                      tttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCaa                                                                                                        >  2:394345/1‑149 (MQ=255)
                       ttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCAtt                                                                                                                          >  4:471407/1‑130 (MQ=255)
                       ttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCAtt                                                                                                                          <  3:471407/130‑1 (MQ=255)
                        tCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAcc                                                                                                      <  3:246810/149‑1 (MQ=255)
                          tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCa                                                                                                                                    >  4:166675/1‑117 (MQ=255)
                          tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCa                                                                                                                                    <  3:166675/117‑1 (MQ=255)
                          tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTg                                                                                                    >  2:455900/1‑149 (MQ=255)
                               cccAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtttt                                                                                               <  3:340981/149‑1 (MQ=255)
                                ccAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTg                                                                                              <  1:292288/149‑1 (MQ=255)
                                 cAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                                                             <  4:305568/149‑1 (MQ=255)
                                        tAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAtt                                                                                      >  4:48677/1‑149 (MQ=255)
                                             gTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTc                                                                                 <  1:455900/149‑1 (MQ=255)
                                              tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCa                                                                                >  2:315812/1‑149 (MQ=255)
                                                      tGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGCTTTGGGTAGATTTGCTCAAATTcgcg                                                                        >  2:148084/1‑149 (MQ=255)
                                                          tCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATAGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGc                                                                    <  1:446842/149‑1 (MQ=255)
                                                             tAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGc                                                                                   >  3:15991/1‑131 (MQ=255)
                                                             tAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGc                                                                                   <  4:15991/131‑1 (MQ=255)
                                                                    gATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa                                                          <  3:48677/149‑1 (MQ=255)
                                                                    gATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGATaa                                                          <  1:394345/149‑1 (MQ=255)
                                                                          tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAGCCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGatat                                                    >  1:400125/1‑149 (MQ=255)
                                                                          tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGatat                                                    >  2:393616/1‑149 (MQ=255)
                                                                                    tCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAAt                                                         <  2:308729/134‑1 (MQ=255)
                                                                                    tCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTAGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCTCTGCGACACGCTAAt                                                         >  1:308729/1‑134 (MQ=255)
                                                                                      ggAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGAt                                        <  3:244839/149‑1 (MQ=255)
                                                                                      ggAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGAt                                        <  4:147392/149‑1 (MQ=255)
                                                                                            gATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTa                                  <  1:393616/149‑1 (MQ=255)
                                                                                               atCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCCTTGGGCGGCCCAGATTCCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGa                               <  2:400125/149‑1 (MQ=255)
                                                                                                tCGGCTTTCGCGAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTAGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGACATTGGCATCGTGATCCATTACGAc                              >  3:314033/1‑149 (MQ=255)
                                                                                                               aCTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggt               <  1:148084/149‑1 (MQ=255)
                                                                                                                cTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg              <  4:197266/149‑1 (MQ=255)
                                                                                                                 ttCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg             >  4:258743/1‑149 (MQ=255)
                                                                                                                     aCCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGcc         <  2:249064/149‑1 (MQ=255)
                                                                                                                          cGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGt    <  4:330841/149‑1 (MQ=255)
                                                                                                                            tGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCg  >  1:457757/1‑149 (MQ=255)
                                                                                                                              gAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCAtt                                   <  4:105747/114‑1 (MQ=255)
                                                                                                                              gAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCAtt                                   >  3:105747/1‑114 (MQ=255)
                                                                                                                                        |                                                                                                                                        
GCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCG  >  NC_000913/4116921‑4117193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: