Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,126,129 A→T 10.2% S228T (TCC→ACC)  priA ← Primosome factor n' (replication factor Y)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,126,1290AT10.2% 154.2 / 8.1 49S228T (TCC→ACC) priAPrimosome factor n' (replication factor Y)
Reads supporting (aligned to +/- strand):  ref base A (23/21);  new base T (2/3);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

TGCAGAACTTCCACGGGGGCATTAAAACGTTCACGAAAACGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTT  >  NC_000913/4125981‑4126275
                                                                                                                                                    |                                                                                                                                                  
tGCAGAACTTCCACGGGGGCATTAAAACGTTCACGAAAACGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGa                                                                                                                                                    >  3:153891/1‑149 (MQ=255)
            aCGGGGGCATTAAAACGTTCACGAAAACGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGTTCCGGTaaaacg                                                                                                                                        <  3:449837/149‑5 (MQ=255)
            aCGGGGGCATTAAAACGTTCACGAAAACGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAAcgcc                                                                                                                                        >  1:355870/1‑149 (MQ=255)
             cGGGGGCATTAAAACGTTCACGAAAACGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAAcgccc                                                                                                                                       <  3:469446/149‑1 (MQ=255)
              gggggCATTAAAACGTTCACGAAAACGGGCGATAGATTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGTTCCGGTaaaacgga                                                                                                                                      <  3:324934/149‑7 (MQ=255)
                gggCATTAAAACGTTCACGAAAACGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAAcgcccgcc                                                                                                                                    >  4:264656/1‑149 (MQ=255)
                        aaaCGTTCACGAAAACGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCa                                                                                                                            >  2:160639/1‑149 (MQ=255)
                              tCACGAAAACGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGa                                                                                                                      >  3:287012/1‑149 (MQ=255)
                               cACGAAAACGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGaa                                                                                                                     >  4:466072/1‑149 (MQ=255)
                                  gAAAACGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGaaaaa                                                                                                                  <  1:14090/149‑1 (MQ=255)
                                  gAAAACGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGaaaaa                                                                                                                  <  3:361641/149‑1 (MQ=255)
                                     aaCGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTa                                                                                                               <  1:124586/149‑1 (MQ=255)
                                       cGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATc                                                                                                             <  4:69557/149‑1 (MQ=255)
                                          gCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGc                                                                                                          <  4:386203/149‑1 (MQ=255)
                                                gTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTAt                                                                                                              <  4:136037/139‑1 (MQ=255)
                                                gTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTAt                                                                                                              >  3:136037/1‑139 (MQ=255)
                                                gTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCt                                                                                                    >  1:306228/1‑149 (MQ=255)
                                                 tttGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTa                                                                                                   >  1:183114/1‑149 (MQ=255)
                                                  ttGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTAt                                                                                                  >  2:265078/1‑149 (MQ=255)
                                                         gtCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTgc                                                                                           <  2:183114/149‑1 (MQ=255)
                                                          tCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTgcg                                                                                          <  3:466072/149‑1 (MQ=255)
                                                              gCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTAt                                                                                                              <  1:48311/125‑1 (MQ=255)
                                                              gCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTAt                                                                                                              >  2:48311/1‑125 (MQ=255)
                                                                   tttCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAAcggcg                                                                                 >  2:181429/1‑149 (MQ=255)
                                                                     tCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGt                                                                               <  2:385308/149‑1 (MQ=255)
                                                                               tCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCg                                                                     <  1:265078/149‑1 (MQ=255)
                                                                                cACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCgcgc                                                                                                     >  1:133918/1‑116 (MQ=255)
                                                                                cACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCgcgc                                                                                                     <  2:133918/116‑1 (MQ=255)
                                                                                  ccAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTa                                                                  >  2:280326/1‑149 (MQ=255)
                                                                                   cAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTAt                                                                 >  2:338461/1‑149 (MQ=255)
                                                                                         cTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATc                                                           <  4:287012/149‑1 (MQ=255)
                                                                                                 ccTGAGCGACCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGc                                                   >  1:408933/1‑149 (MQ=255)
                                                                                                      gcgagcACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAg                                                                                                                           <  4:58756/72‑1 (MQ=255)
                                                                                                      gcgagcACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAg                                                                                                                           >  3:58756/1‑72 (MQ=255)
                                                                                                              gTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACgg                                      >  2:178949/1‑149 (MQ=255)
                                                                                                              gTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACgg                                      >  2:376693/1‑149 (MQ=255)
                                                                                                                    cAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAgt                                <  1:160639/149‑1 (MQ=255)
                                                                                                                      gTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAgttc                              >  3:335393/1‑149 (MQ=255)
                                                                                                                        aCGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAgttcgt                            <  1:338461/149‑1 (MQ=255)
                                                                                                                         cGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAgttcgtt                           >  2:14914/1‑149 (MQ=255)
                                                                                                                                aTAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGt                    >  2:157507/1‑149 (MQ=255)
                                                                                                                                   aaCCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGc                 <  1:178949/149‑1 (MQ=255)
                                                                                                                                   aaCCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGc                 <  2:355870/149‑1 (MQ=255)
                                                                                                                                      cTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTaa              >  4:250133/1‑149 (MQ=255)
                                                                                                                                      cTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTaa              >  2:31146/1‑149 (MQ=255)
                                                                                                                                            gttACCGGTTCCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTa                                                                  >  3:287763/2‑91 (MQ=255)
                                                                                                                                            gttACCGGTTCCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTa                                                                  <  4:287763/90‑1 (MQ=255)
                                                                                                                                            gttACCGGTTCCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTg        >  3:328998/2‑149 (MQ=255)
                                                                                                                                              tACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGgt      <  1:376693/149‑1 (MQ=255)
                                                                                                                                                  ggAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGttt  <  3:264656/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TGCAGAACTTCCACGGGGGCATTAAAACGTTCACGAAAACGGGCGATAGTTTGCGGTGTCAGGCCGATTTCCGGCACCATCACCAGCGCCTGTTTGCCCTGAGCGAGCACGTTTTCCAGTACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTT  >  NC_000913/4125981‑4126275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑