Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,270,142 | G→A | 9.2% | intergenic (+15/‑96) | aphA → / → yjbQ | acid phosphatase/phosphotransferase, class B, non‑specific/thiamine phosphate synthase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,270,142 | 0 | G | A | 9.2% | 196.6 / 9.6 | 65 | intergenic (+15/‑96) | aphA/yjbQ | acid phosphatase/phosphotransferase, class B, non‑specific/thiamine phosphate synthase |
Reads supporting (aligned to +/- strand): ref base G (28/31); new base A (2/4); total (30/35) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.78e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01 |
AATGATATTACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCA > NC_000913/4269996‑4270287 | aaTGATATTACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTg > 1:115576/1‑149 (MQ=255) aTGATATTACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGa > 4:33860/1‑149 (MQ=255) tatTACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGct < 3:23265/149‑1 (MQ=255) aCCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCATCACGCTCTcc < 2:331684/149‑1 (MQ=255) aCCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCATCACGCTCTcc < 1:302755/149‑1 (MQ=255) cACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGc > 4:260911/1‑149 (MQ=255) gcTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTTAAGAGGTGATCGTCAATTCAGAATACTGACAGAGAGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATca > 4:389088/1‑149 (MQ=255) tCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATcaca > 1:301717/1‑149 (MQ=255) tCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATcaca < 4:221132/149‑1 (MQ=255) tCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCATGCCACAAACGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCacac < 3:409626/142‑1 (MQ=255) tCCGCATTCGGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCacac > 4:409626/1‑142 (MQ=255) gCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATc < 1:32467/146‑1 (MQ=255) gCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATc > 2:32467/1‑146 (MQ=255) cATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCttt < 3:223924/149‑1 (MQ=255) cTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCt < 2:115576/149‑1 (MQ=255) gcgcCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCt < 3:195792/149‑1 (MQ=255) tCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCttt > 1:156048/1‑136 (MQ=255) tCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCttt < 2:156048/136‑1 (MQ=255) tCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCt < 1:216275/144‑1 (MQ=255) tCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCt > 2:216275/1‑144 (MQ=255) ctacAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTa > 1:237902/1‑149 (MQ=255) cAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCaaa > 2:317262/1‑139 (MQ=255) cAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCaaa < 1:317262/139‑1 (MQ=255) cAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAaga < 3:17181/149‑1 (MQ=255) aaCCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAagaga < 4:207727/149‑1 (MQ=255) aaCCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAagaga < 3:260911/149‑1 (MQ=255) cccTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGt > 4:398489/1‑149 (MQ=255) cTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACATAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTtgct > 2:388519/1‑128 (MQ=255) cTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGGGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTtgct < 1:388519/128‑1 (MQ=255) cTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTAt > 2:450540/1‑111 (MQ=255) cTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTAt < 1:450540/111‑1 (MQ=255) cTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGttt > 4:296341/1‑149 (MQ=255) cTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGttt > 1:82448/1‑149 (MQ=255) tGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGTTTAAGAGAGTTTTa < 3:33860/149‑1 (MQ=255) tGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTa < 4:454280/149‑1 (MQ=255) tGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTa < 2:301717/149‑1 (MQ=255) acaAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCaa > 2:252315/1‑149 (MQ=255) acaAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCaa > 2:6686/1‑149 (MQ=255) gCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGag < 3:296341/149‑1 (MQ=255) gCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGag < 1:211822/149‑1 (MQ=255) ggTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGAtt > 1:421809/1‑126 (MQ=255) ggTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGAtt < 2:421809/126‑1 (MQ=255) aaGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATc > 1:257392/1‑149 (MQ=255) gTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTa < 3:225211/114‑1 (MQ=255) gTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTa > 4:225211/1‑114 (MQ=255) gTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTc > 3:374961/1‑149 (MQ=255) tCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTc < 4:442854/79‑1 (MQ=255) tCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTc > 3:442854/1‑79 (MQ=255) aTTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGc > 2:357013/1‑149 (MQ=255) aaTACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGt > 3:456217/1‑100 (MQ=255) aaTACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGt < 4:456217/100‑1 (MQ=255) agagCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAAtctc > 4:111492/1‑149 (MQ=255) gagCGGGAGAGCGTGATGCTCTCCCGAATGCTGTATTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCg < 3:389088/149‑1 (MQ=255) ttcGGGAGAGCATCACGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGt > 1:123961/3‑149 (MQ=255) agCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGt < 3:111492/149‑1 (MQ=255) gCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTg < 2:82448/149‑1 (MQ=255) cGGGAGAGCATCACGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTgg < 2:123961/149‑1 (MQ=255) gggAGAGCATCACGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAaga < 3:301334/82‑1 (MQ=39) gggAGAGCATCACGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAaga > 4:301334/1‑82 (MQ=39) ggAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGt > 1:123457/1‑149 (MQ=255) gagaGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGtt < 3:246520/149‑1 (MQ=255) agagCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGttt < 1:357013/149‑1 (MQ=255) agCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCa < 3:168888/149‑1 (MQ=255) cGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTtgc > 4:230467/1‑59 (MQ=255) cGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTtgc < 3:230467/59‑1 (MQ=255) | AATGATATTACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCA > NC_000913/4269996‑4270287 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |