Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,270,146 T→C 9.0% intergenic (+19/‑92) aphA → / → yjbQ acid phosphatase/phosphotransferase, class B, non‑specific/thiamine phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,270,1460TC 9.0% 202.4 / 6.9 65intergenic (+19/‑92)aphA/yjbQacid phosphatase/phosphotransferase, class B, non‑specific/thiamine phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (27/32);  new base C (2/4);  total (29/36)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGG  >  NC_000913/4270001‑4270292
                                                                                                                                                 |                                                                                                                                                  
tatTACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGct                                                                                                                                                 <  3:23265/149‑1 (MQ=255)
    aCCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCATCACGCTCTcc                                                                                                                                             <  2:331684/149‑1 (MQ=255)
    aCCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCATCACGCTCTcc                                                                                                                                             <  1:302755/149‑1 (MQ=255)
           cACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGc                                                                                                                                      >  4:260911/1‑149 (MQ=255)
                         gcTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTTAAGAGGTGATCGTCAATTCAGAATACTGACAGAGAGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATca                                                                                                                        >  4:389088/1‑149 (MQ=255)
                           tCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATcaca                                                                                                                      <  4:221132/149‑1 (MQ=255)
                           tCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATcaca                                                                                                                      >  1:301717/1‑149 (MQ=255)
                                   tCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCATGCCACAAACGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCacac                                                                                                                     <  3:409626/142‑1 (MQ=255)
                                   tCCGCATTCGGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCacac                                                                                                                     >  4:409626/1‑142 (MQ=255)
                                      gCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATc                                                                                                              >  2:32467/1‑146 (MQ=255)
                                      gCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATc                                                                                                              <  1:32467/146‑1 (MQ=255)
                                       cATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCttt                                                                                                          <  3:223924/149‑1 (MQ=255)
                                           cTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCt                                                                                                      <  2:115576/149‑1 (MQ=255)
                                               gcgcCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCt                                                                                                  <  3:195792/149‑1 (MQ=255)
                                                    tCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCttt                                                                                                          >  1:156048/1‑136 (MQ=255)
                                                    tCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCttt                                                                                                          <  2:156048/136‑1 (MQ=255)
                                                    tCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCt                                                                                                  <  1:216275/144‑1 (MQ=255)
                                                    tCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCt                                                                                                  >  2:216275/1‑144 (MQ=255)
                                                               ctacAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTa                                                                                  >  1:237902/1‑149 (MQ=255)
                                                                  cAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCaaa                                                                                         <  1:317262/139‑1 (MQ=255)
                                                                  cAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCaaa                                                                                         >  2:317262/1‑139 (MQ=255)
                                                                  cAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAaga                                                                               <  3:17181/149‑1 (MQ=255)
                                                                    aaCCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAagaga                                                                             <  3:260911/149‑1 (MQ=255)
                                                                    aaCCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAagaga                                                                             <  4:207727/149‑1 (MQ=255)
                                                                      cccTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGt                                                                           >  4:398489/1‑149 (MQ=255)
                                                                        cTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACATAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTtgct                                                                                              >  2:388519/1‑128 (MQ=255)
                                                                        cTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGGGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTtgct                                                                                              <  1:388519/128‑1 (MQ=255)
                                                                        cTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTAt                                                                                                               <  1:450540/111‑1 (MQ=255)
                                                                        cTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTAt                                                                                                               >  2:450540/1‑111 (MQ=255)
                                                                        cTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGttt                                                                         >  4:296341/1‑149 (MQ=255)
                                                                        cTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGttt                                                                         >  1:82448/1‑149 (MQ=255)
                                                                          tGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGTTTAAGAGAGTTTTa                                                                       <  3:33860/149‑1 (MQ=255)
                                                                          tGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTa                                                                       <  4:454280/149‑1 (MQ=255)
                                                                          tGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTa                                                                       <  2:301717/149‑1 (MQ=255)
                                                                              acaAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCaa                                                                   >  2:6686/1‑149 (MQ=255)
                                                                              acaAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCaa                                                                   >  2:252315/1‑149 (MQ=255)
                                                                                  gCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGag                                                               <  1:211822/149‑1 (MQ=255)
                                                                                  gCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGag                                                               <  3:296341/149‑1 (MQ=255)
                                                                                     ggTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGAtt                                                                                   <  2:421809/126‑1 (MQ=255)
                                                                                     ggTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGAtt                                                                                   >  1:421809/1‑126 (MQ=255)
                                                                                                  aaGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATc                                               >  1:257392/1‑149 (MQ=255)
                                                                                                             gTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTa                                                                       <  3:225211/114‑1 (MQ=255)
                                                                                                             gTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTa                                                                       >  4:225211/1‑114 (MQ=255)
                                                                                                             gTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTc                                    >  3:374961/1‑149 (MQ=255)
                                                                                                              tCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTc                                                                                                         >  3:442854/1‑79 (MQ=255)
                                                                                                              tCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTc                                                                                                         <  4:442854/79‑1 (MQ=255)
                                                                                                                 aTTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGc                                >  2:357013/1‑149 (MQ=255)
                                                                                                                       aaTACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGt                                                                           <  4:456217/100‑1 (MQ=255)
                                                                                                                       aaTACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGt                                                                           >  3:456217/1‑100 (MQ=255)
                                                                                                                                agagCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAAtctc                 >  4:111492/1‑149 (MQ=255)
                                                                                                                                 gagCGGGAGAGCGTGATGCTCTCCCGAATGCTGTATTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCg                <  3:389088/149‑1 (MQ=255)
                                                                                                                                  ttcGGGAGAGCATCACGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGt               >  1:123961/3‑149 (MQ=255)
                                                                                                                                  agCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGt               <  3:111492/149‑1 (MQ=255)
                                                                                                                                   gCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTg              <  2:82448/149‑1 (MQ=255)
                                                                                                                                    cGGGAGAGCATCACGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTgg             <  2:123961/149‑1 (MQ=255)
                                                                                                                                     gggAGAGCATCACGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAaga                                                                               <  3:301334/82‑1 (MQ=39)
                                                                                                                                     gggAGAGCATCACGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAaga                                                                               >  4:301334/1‑82 (MQ=39)
                                                                                                                                      ggAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGt           >  1:123457/1‑149 (MQ=255)
                                                                                                                                       gagaGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGtt          <  3:246520/149‑1 (MQ=255)
                                                                                                                                        agagCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGttt         <  1:357013/149‑1 (MQ=255)
                                                                                                                                          agCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCa       <  3:168888/149‑1 (MQ=255)
                                                                                                                                            cGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTtgc                                                                                               >  4:230467/1‑59 (MQ=255)
                                                                                                                                            cGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTtgc                                                                                               <  3:230467/59‑1 (MQ=255)
                                                                                                                                               gATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTgg  <  3:403771/149‑1 (MQ=255)
                                                                                                                                               gATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTgg  >  1:396195/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TATTACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGG  >  NC_000913/4270001‑4270292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: