Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,470,504 G→C 8.2% intergenic (+183/+23) mgtA → / ← ridA magnesium transporter/enamine/imine deaminase, reaction intermediate detoxification

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,470,5040GC 8.2% 168.2 / 6.6 49intergenic (+183/+23)mgtA/ridAmagnesium transporter/enamine/imine deaminase, reaction intermediate detoxification
Reads supporting (aligned to +/- strand):  ref base G (20/25);  new base C (2/2);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.02e-01

TTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTGAAGAAGGCTTCGTAAGTG  >  NC_000913/4470371‑4470647
                                                                                                                                      |                                                                                                                                               
ttttGTTCATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTcc                                                                                                                                  <  1:418406/149‑1 (MQ=255)
ttttGTTCATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTcc                                                                                                                                  >  2:418406/1‑149 (MQ=255)
     ttCATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCaa                                                                                                                             <  2:54363/149‑1 (MQ=255)
       cATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGa                                                                                                                           <  2:396621/149‑1 (MQ=255)
        aTGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGCTATCAT‑GCCCGGAttaa                                                                                                                                   <  1:439962/140‑3 (MQ=37)
        aTGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGCTATCAT‑GCCCGGAttaa                                                                                                                                   >  2:439962/1‑138 (MQ=37)
            cGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGc                                                                                                                      <  4:414345/149‑1 (MQ=255)
               aTGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGACAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGcgac                                                                                                                   <  3:244542/149‑1 (MQ=255)
                  cggcgTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGaa                                                                                                                <  3:248059/149‑1 (MQ=255)
                  cggcgTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGaa                                                                                                                <  4:317224/149‑1 (MQ=255)
                      gTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGc                                                                                                            >  2:94847/1‑149 (MQ=255)
                              cTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGCTATCAT‑GCCCGGAtta                                                                                                                                    <  1:301702/117‑2 (MQ=25)
                              cTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGCTATCAT‑GCCCGGAtta                                                                                                                                    >  2:301702/1‑116 (MQ=25)
                                         aacaaATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATc                                                                                         >  4:441425/1‑149 (MQ=255)
                                         aacaaATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATc                                                                                         >  3:462800/1‑149 (MQ=255)
                                               ttCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGcc                                                                                                                                           >  1:459189/1‑93 (MQ=255)
                                               ttCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGcc                                                                                                                                           <  2:459189/93‑1 (MQ=255)
                                                  aaTAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCt                                                                                >  3:426188/1‑149 (MQ=255)
                                                    tAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCa                                                                                     <  2:300259/142‑1 (MQ=255)
                                                    tAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCa                                                                                     >  1:300259/1‑142 (MQ=255)
                                                     aaaTTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTc                                                                             <  4:426188/149‑1 (MQ=255)
                                                       aTTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCgg                                                                           <  2:192525/149‑1 (MQ=255)
                                                                   aCGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAAc                                                               <  2:65719/149‑1 (MQ=255)
                                                                     gTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACtt                                                             <  1:94847/149‑1 (MQ=255)
                                                                         gCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAAc                                                         >  2:391407/1‑149 (MQ=255)
                                                                         gCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAAc                                                         >  2:48386/1‑149 (MQ=255)
                                                                          ccTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACg                                                        >  1:446703/1‑149 (MQ=255)
                                                                          ccTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACg                                                        >  2:181605/1‑149 (MQ=255)
                                                                                  gCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACg                                                <  3:410110/149‑1 (MQ=255)
                                                                                       gcgcATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCg                                           <  1:372395/149‑1 (MQ=255)
                                                                                            tCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAg                                      >  3:312388/1‑149 (MQ=255)
                                                                                                   aTTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGt                               <  4:462800/149‑1 (MQ=255)
                                                                                                              gTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTg                    <  4:312388/149‑1 (MQ=255)
                                                                                                                   caGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTgaagaa               >  3:56680/1‑149 (MQ=255)
                                                                                                                   caGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTgaagaa               <  4:104434/149‑1 (MQ=255)
                                                                                                                    aGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGc                                                                                                      <  3:350606/61‑1 (MQ=255)
                                                                                                                    aGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGc                                                                                                      >  4:350606/1‑61 (MQ=255)
                                                                                                                    aGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTGaagaag              >  3:218430/1‑149 (MQ=255)
                                                                                                                     gACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTGAAGAAgg             <  1:181605/149‑1 (MQ=255)
                                                                                                                      aCTTAATCCGAGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCTAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTGAAGAAGGc            >  4:309389/1‑149 (MQ=255)
                                                                                                                       cTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGt                     >  1:246953/1‑139 (MQ=255)
                                                                                                                       cTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGt                     <  2:246953/139‑1 (MQ=255)
                                                                                                                        ttAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTGAAGAAGGCtt          >  3:436067/1‑149 (MQ=255)
                                                                                                                        ttAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTGAAGAAGGCtt          <  4:218430/149‑1 (MQ=255)
                                                                                                                        ttAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTGAAGAAGGCtt          >  1:277410/1‑149 (MQ=255)
                                                                                                                               gggC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGt                                               >  4:261572/1‑105 (MQ=255)
                                                                                                                               gggC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGt                                               <  3:261572/105‑1 (MQ=255)
                                                                                                                                ggC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTGAAGAAGGCTTCGTAAGTg  <  1:48386/149‑1 (MQ=255)
                                                                                                                                ggC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTGAAGAAGGCTTCGTAAGTg  <  3:309389/149‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                               
TTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTGAAGAAGGCTTCGTAAGTG  >  NC_000913/4470371‑4470647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑