Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,501,229 A→G 9.4% intergenic (‑438/‑31) yjgX ← / → yjgZ pseudogene fragment/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,501,2290AG 9.4% 213.7 / 9.0 73intergenic (‑438/‑31)yjgX/yjgZpseudogene fragment/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (30/36);  new base G (2/5);  total (32/41)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGGGTATGACCGATGCCTTTCTCACTCTCTTCCCGGTCATCGTCGGCCTTCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAG  >  NC_000913/4501081‑4501374
                                                                                                                                                    |                                                                                                                                                 
ggTGGGTATGACCGATGCCTTTCTCACTCTCTTCCCGGTCATCGTCGGCCTTCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTgaa                                                                                                                                                   <  4:110544/149‑1 (MQ=255)
      tATGACCGATGCCTTTCTCACTCTCTTCCCGGTCATCGTCGGCCTTCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCTTCAGGAAGTc                                                                                                                                             <  2:261130/149‑1 (MQ=255)
      tATGACCGATGCCTTTCTCACTCTCTTCCCGGTCATCGTCGGCCTTCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCTTCAGGAAGTc                                                                                                                                             <  1:83651/149‑1 (MQ=255)
        tGACCGATGCCTTTCTCACTCTCTTCCCGGTCATCGTCGGCCTTCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGt                                                                                                                                           >  3:351372/1‑149 (MQ=255)
         gACCGATGCCTTTCTCACTCTCTTCCCGGTCATCGTCGGCCTTCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCTTCAGGAAGTcaac                                                                                                                                          <  4:185142/149‑4 (MQ=255)
         gACCGATGCCTTTCTCACTCTCTTCCCGGTCATCGTCGGCCTTCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGtt                                                                                                                                          <  3:205590/149‑1 (MQ=255)
             gATGCCTTTCTCACTCTCTTCCCGGTCATCGTCGGCCTTCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAg                                                                                                                                      <  3:443759/149‑1 (MQ=255)
              aTGCCTTTCTCACTCTCTTCCCGGTCATCGTCGGCCTTCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAgg                                                                                                                                     <  4:351372/149‑1 (MQ=255)
              aTGCCTTTCTCACTCTCTTCCCGGTCATCGTCGGCCTTCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAgg                                                                                                                                     <  2:429576/149‑1 (MQ=255)
                 ccTTTCTCACTCTCTTCCCGGTCATCGTCGGCCTTCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGgag                                                                                                                                  >  3:183905/1‑149 (MQ=255)
                        cACTCTCTTCCCGGTCATCGTCGGCCTTCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCa                                                                                                                           <  4:456882/149‑1 (MQ=255)
                         aCTCTCTTCCCGGTCATCGTCGGCCTTCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGTGAGATTCAg                                                                                                                          >  2:224825/1‑149 (MQ=255)
                          ctctctTCCCGGTCATCGTCGGCCTTCACCGTTATTGTGGCGTGTCAGCACCCTCGAAGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTAAGc                                                                                                                         >  2:381161/1‑149 (MQ=255)
                           tctctTCCCGGTCATCGTCGGCCTTCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGcc                                                                                                                        >  2:386802/1‑149 (MQ=255)
                             tctTCCCGGTCATCGTCGGCCTTCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAg                                                                                                                                      <  4:144404/133‑1 (MQ=255)
                             tctTCCCGGTCATCGTCGGCCTTCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAg                                                                                                                                      >  3:144404/1‑133 (MQ=255)
                               ttCCCGGTCATCGTCGGCCTTCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCttt                                                                                                                    <  1:93718/149‑1 (MQ=255)
                                tCCCGGTCATCGTCGGCCTTCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCtttt                                                                                                                   <  3:248336/149‑1 (MQ=255)
                                   cGGTCATCGTCTGCCTTCCCCTGTATTGTGGCGTGTCAGCACACTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGtt                                                                                                                <  4:142084/149‑1 (MQ=255)
                                      tCATCGTCGGCCGTCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTAcc                                                                                                             <  2:379569/149‑1 (MQ=255)
                                         tcgtcgGCCTTCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTAcctcc                                                                                                          <  4:151475/149‑1 (MQ=255)
                                                  ttCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGct                                                                                                 >  1:319691/1‑149 (MQ=255)
                                                    ccccGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGcttc                                                                                               <  4:22175/149‑1 (MQ=255)
                                                    ccccGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGcttc                                                                                               <  1:386802/149‑1 (MQ=255)
                                                     cccGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGcttct                                                                                              >  3:134071/1‑149 (MQ=255)
                                                      ccGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGgagaga                                                                                                                               <  1:406183/115‑1 (MQ=255)
                                                      ccGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGgagaga                                                                                                                               >  2:406183/1‑115 (MQ=255)
                                                          tATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTcc                                                                                         <  2:64984/149‑1 (MQ=255)
                                                             tgtgGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTc                                                                                      <  3:143373/149‑1 (MQ=255)
                                                              gtgGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCAGAACCCGTTGACTTCCTGAGAAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTACTTcc                                                                                     <  4:302612/149‑1 (MQ=255)
                                                                   gtgtCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGt                                                                                                                                           <  1:312270/90‑1 (MQ=255)
                                                                   gtgtCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGt                                                                                                                                           >  2:312270/1‑90 (MQ=255)
                                                                   gtgtCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCg                                                                                >  3:188198/1‑149 (MQ=255)
                                                                         gCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCga                                                                          <  4:188198/149‑1 (MQ=255)
                                                                           aCCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCgaga                                                                        <  3:252113/149‑1 (MQ=255)
                                                                             ccTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAg                                                                                                                          >  2:331738/1‑97 (MQ=255)
                                                                             ccTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAg                                                                                                                          <  1:331738/97‑1 (MQ=255)
                                                                                   cGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTgaagaa                                                                                                                                                >  4:277854/1‑69 (MQ=255)
                                                                                   cGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTgaagaa                                                                                                                                                <  3:277854/69‑1 (MQ=255)
                                                                                   cGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCg                                                                <  3:116937/149‑1 (MQ=255)
                                                                                      cATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCgggg                                                             <  3:339222/149‑1 (MQ=255)
                                                                                       aTTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGAtt                                                                                                                             >  1:176427/1‑84 (MQ=255)
                                                                                       aTTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGAtt                                                                                                                             <  2:176427/84‑1 (MQ=255)
                                                                                       aTTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGc                                                            >  1:343966/1‑149 (MQ=255)
                                                                                       aTTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGc                                                            <  2:268988/149‑1 (MQ=255)
                                                                                           cGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGAc                                                        <  4:171554/149‑1 (MQ=255)
                                                                                                  tgctCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCAcc                                                 >  3:215675/1‑149 (MQ=255)
                                                                                                  tgctCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCAcc                                                 >  3:348731/1‑149 (MQ=255)
                                                                                                       aGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACgg                                            >  2:54086/1‑149 (MQ=255)
                                                                                                          gtTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGaaa                                         >  1:454559/1‑149 (MQ=255)
                                                                                                          gtTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGATTCAGGGACAGCGTCAGCCCTCTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGGTAGTTAGTCGGGGCGGACTTTCACTGACGGaaa                                         >  4:56053/1‑149 (MQ=255)
                                                                                                           ttCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAg                                        <  4:215675/149‑1 (MQ=255)
                                                                                                           ttCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTACTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAg                                        >  3:399434/1‑149 (MQ=255)
                                                                                                             cactcacGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGAt                                      <  2:319691/149‑1 (MQ=255)
                                                                                                              actcacGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATc                                     >  3:182191/1‑149 (MQ=255)
                                                                                                                tcacGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGct                                                                                                 >  1:233756/1‑87 (MQ=255)
                                                                                                                tcacGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGt                                   <  1:54086/149‑1 (MQ=255)
                                                                                                                tcacGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTGACCTCCTGGACCGct                                                                                                 <  2:233756/87‑1 (MQ=255)
                                                                                                                      tACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCAttt                             <  3:39292/149‑1 (MQ=255)
                                                                                                                          gCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAg                         <  2:454559/149‑1 (MQ=255)
                                                                                                                          gCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAg                         <  4:20181/149‑1 (MQ=255)
                                                                                                                          gCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAg                         <  1:359973/149‑1 (MQ=255)
                                                                                                                           cccGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAgg                        >  4:423668/1‑149 (MQ=255)
                                                                                                                                 tAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGcacaca                  >  4:180042/1‑149 (MQ=255)
                                                                                                                                    cccGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTc                                                    >  3:458353/1‑112 (MQ=255)
                                                                                                                                    cccGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTc                                                    <  4:458353/112‑1 (MQ=255)
                                                                                                                                       aacgACTTCTTCAGGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACAcaacaa            >  3:383957/4‑149 (MQ=255)
                                                                                                                                          gACTTCTTCAGGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCg                                                           >  3:297532/1‑99 (MQ=255)
                                                                                                                                          gACTTCTTCAGGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCg                                                           <  4:297532/99‑1 (MQ=255)
                                                                                                                                           aCTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACacaa               >  2:286204/1‑142 (MQ=255)
                                                                                                                                           aCTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACacaa               <  1:286204/142‑1 (MQ=255)
                                                                                                                                           aCTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATc        >  4:382096/1‑149 (MQ=255)
                                                                                                                                             ttCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTcc                                                                                         >  2:424519/1‑66 (MQ=255)
                                                                                                                                             ttCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTcc                                                                                         <  1:424519/66‑1 (MQ=255)
                                                                                                                                                 tGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAg  >  3:299408/1‑149 (MQ=255)
                                                                                                                                                 tGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAg  >  4:112167/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GGTGGGTATGACCGATGCCTTTCTCACTCTCTTCCCGGTCATCGTCGGCCTTCCCCGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAG  >  NC_000913/4501081‑4501374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: