Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,551,429 C→T 5.9% intergenic (‑133/‑207) gntP ← / → uxuA fructuronate transporter/mannonate hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,551,4290CT 5.9% 221.5 / 2.2 67intergenic (‑133/‑207)gntP/uxuAfructuronate transporter/mannonate hydrolase
Reads supporting (aligned to +/- strand):  ref base C (31/32);  new base T (2/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAA  >  NC_000913/4551284‑4551576
                                                                                                                                                 |                                                                                                                                                   
tAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGcaac                                                                                                                                                  <  1:395623/149‑1 (MQ=255)
tAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAAATTGCAGcaaa                                                                                                                                                  <  1:313885/149‑2 (MQ=255)
   gCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCaacaac                                                                                                                                               >  3:293723/1‑149 (MQ=255)
           aTAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGagag                                                                                                                                       >  2:151220/1‑149 (MQ=255)
            tAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGt                                                                                                                                      >  4:242773/1‑149 (MQ=255)
             aaCGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTg                                                                                                                                     >  4:378878/1‑149 (MQ=255)
                     accttacctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCtt                                                                                                                             <  1:406317/149‑1 (MQ=255)
                       cttacctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTtgt                                                                                                                           >  1:276562/1‑149 (MQ=255)
                            ctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGAtgt                                                                                                                      <  1:151220/149‑1 (MQ=255)
                                  gAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTaa                                                                                                                <  4:406612/149‑1 (MQ=255)
                                    aCCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAAcc                                                                                                              >  2:70890/1‑149 (MQ=255)
                                     ccGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCa                                                                                                             >  4:227291/1‑149 (MQ=255)
                                       gttgttATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAAt                                                                                                           >  2:209804/1‑149 (MQ=255)
                                              ttttGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGaa                                                                                                    <  2:152984/149‑1 (MQ=255)
                                                 tGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTc                                                                                                 <  2:132762/149‑1 (MQ=255)
                                                     cGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGt                                                                                             <  4:430423/149‑1 (MQ=255)
                                                     cGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGt                                                                                             >  3:430423/1‑149 (MQ=255)
                                                      gATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGtt                                                                                            >  2:396540/1‑149 (MQ=255)
                                                      gATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGtt                                                                                            <  3:473329/149‑1 (MQ=255)
                                                           gttatgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACAt                                                                                       <  2:276562/149‑1 (MQ=255)
                                                            ttatgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATg                                                                                      >  1:159170/1‑149 (MQ=255)
                                                               tgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCt                                                                                   <  1:276749/149‑1 (MQ=255)
                                                                  aaaTTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTAc                                                                                <  3:57322/149‑1 (MQ=255)
                                                                    aTTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCa                                                                              >  2:363469/1‑149 (MQ=255)
                                                                     ttGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCaa                                                                             >  2:70927/1‑149 (MQ=255)
                                                                       ggTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCaaaa                                                                           <  3:378878/149‑1 (MQ=255)
                                                                         tCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAgg                                                                         >  4:174821/1‑149 (MQ=255)
                                                                          cAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTCTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGt                                                                        <  3:242773/149‑1 (MQ=255)
                                                                           aaCCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTaa                                                                                                                <  2:101182/108‑1 (MQ=255)
                                                                           aaCCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTaa                                                                                                                >  1:101182/1‑108 (MQ=255)
                                                                           aaCCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTa                                                                       >  2:243103/1‑149 (MQ=255)
                                                                              cATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGaa                                                                    <  1:243103/149‑1 (MQ=255)
                                                                               aTTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAAc                                                                   <  4:46225/149‑1 (MQ=255)
                                                                                        aTGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGcc                                                          >  2:415592/1‑149 (MQ=255)
                                                                                         tGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCa                                                         >  3:338555/1‑149 (MQ=255)
                                                                                           aaTGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCAtc                                                       >  1:126889/1‑149 (MQ=255)
                                                                                           aaTGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCAtc                                                       <  3:174821/149‑1 (MQ=255)
                                                                                                cacaTCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATc                                                  <  1:209804/149‑1 (MQ=255)
                                                                                                 acaTCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGTAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATcc                                                 >  1:20644/1‑149 (MQ=255)
                                                                                                 acaTCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGTAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATcc                                                 >  1:463588/1‑149 (MQ=255)
                                                                                                       tctGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTg                                                                                           <  2:302108/101‑1 (MQ=255)
                                                                                                       tctGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTg                                                                                           >  1:302108/1‑101 (MQ=255)
                                                                                                        ctGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCaa                                          <  2:104874/149‑1 (MQ=255)
                                                                                                          gATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACg                                        >  2:453225/1‑149 (MQ=255)
                                                                                                              aaTAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGaa                                                                    >  4:466550/1‑117 (MQ=255)
                                                                                                              aaTAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGaa                                                                    >  2:361379/1‑117 (MQ=255)
                                                                                                              aaTAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCAc                                    >  1:147711/1‑149 (MQ=255)
                                                                                                              aaTAACCATCGATTACCCTTTGCTGCAATGTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAAACAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGaa                                                                    <  3:466550/117‑1 (MQ=255)
                                                                                                              aaTAACCATCGACTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGaa                                                                    <  1:361379/117‑1 (MQ=255)
                                                                                                                  aCCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCt                                <  3:227291/149‑1 (MQ=255)
                                                                                                                  aCCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCt                                <  2:159170/149‑1 (MQ=255)
                                                                                                                     aTCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAAttt                                                                                                         <  1:292711/73‑1 (MQ=255)
                                                                                                                     aTCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAAttt                                                                                                         >  2:292711/1‑73 (MQ=255)
                                                                                                                      tCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCgg                            <  1:70927/149‑1 (MQ=255)
                                                                                                                             cccTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgtt                     <  1:415592/149‑1 (MQ=255)
                                                                                                                              ccTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgttg                    >  3:248749/1‑149 (MQ=255)
                                                                                                                               cTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgttgt                   <  4:12103/149‑1 (MQ=255)
                                                                                                                                 ttGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTc                 <  1:396540/149‑1 (MQ=255)
                                                                                                                                 ttGCTGCAAATTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGGCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTc                 >  4:283127/1‑149 (MQ=255)
                                                                                                                                     tgcAAATTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTAc                                                                                >  2:77686/1‑82 (MQ=255)
                                                                                                                                     tgcAAATTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTAc                                                                                <  1:77686/82‑1 (MQ=255)
                                                                                                                                      gcAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCg            <  4:338555/149‑1 (MQ=255)
                                                                                                                                       cAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTTATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGTTCTGGTTGTTCATCCgg           >  1:421146/1‑149 (MQ=255)
                                                                                                                                        aaattGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGa          <  3:283127/146‑1 (MQ=255)
                                                                                                                                        aaTTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGa          <  1:70890/149‑1 (MQ=255)
                                                                                                                                          tttGCAGTAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATa        <  2:20644/149‑1 (MQ=255)
                                                                                                                                          tttGCAGTAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATa        <  2:463588/149‑1 (MQ=255)
                                                                                                                                          tttGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATa        >  1:373685/1‑149 (MQ=255)
                                                                                                                                          tttGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATa        >  4:461556/1‑149 (MQ=255)
                                                                                                                                             gcagcaACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCt     >  4:245684/1‑149 (MQ=255)
                                                                                                                                                gcaACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGACTTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTaaa  <  4:293723/149‑1 (MQ=255)
                                                                                                                                                gcaACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCgg           >  1:372917/1‑140 (MQ=255)
                                                                                                                                                gcaACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCgg           <  2:372917/140‑1 (MQ=255)
                                                                                                                                                gcaACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTaaa  <  3:245684/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAA  >  NC_000913/4551284‑4551576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: