Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,581,139	G→A	10.0%	C108C (TGC→TGT)	hsdS ←	specificity determinant for hsdM and hsdR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,581,139	0	G	A	10.0%	105.5 / 4.3	40	C108C (TGC→TGT)	hsdS	specificity determinant for hsdM and hsdR
Reads supporting (aligned to +/- strand):  ref base G (19/17);  new base A (2/2);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

GTATATTTATCAAATCAAAGCTTGCCGGCTTAATATTATTAATATTTGCACCAGCAGAAAGTGATGAAATTTTGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGACATTGCAATAACAATATCTTCAGGAGATATTTTTTGACTTTCTTTAACAAGATTTTTAGGAACAAA  >  NC_000913/4580991‑4581282                                                                                                                                                     |                                                                                                                                                gTATATTTATCAAATCAAAGCTTGCCGGCTTAATATTATTAATATTTGCACCAGCAGAAAGTGATGAAATTTTGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCg                                                                                                                                                 >  4:248803/1‑149 (MQ=255)    tatTTATCAAATCAAAGCTTGCCGGCTTAATATTATTAATATTTGCACCGGCAGAAAGTGATGAAATTTTGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCaa                                                                                                                                              >  1:278264/1‑149 (MQ=255)      tttATCAAATCAAAGCTTGCCGGCTTAATATTATTAATATTTGCACCAGCAGAAAGTGATGAAATTTTGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCaaaa                                                                                                                                            >  3:382157/1‑149 (MQ=255)       ttATCAAATCAAAGCTTGCCGGCTTAATATTATTAATATTTGCACCAGCAGAAAGTGATGAAATTTTGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAAt                                                                                                                                           <  3:174755/149‑1 (MQ=255)        tATCAAATCAAAGCTTGCCGGCTTAATATTATTAATATTTGCACCAGCAGAAAGTGATGAAATTTTGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATg                                                                                                                                          >  3:311173/1‑149 (MQ=255)         atcaaatcaaAGCTTGCCGGCTTAATATTATTAATATTTGCACCAGCAGAAAGTGATGAAATTTTGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCACAAAATgc                                                                                                                                         >  4:198126/1‑149 (MQ=255)            aaatcaaaGCTTGCCGGCTTAATATTATTAATATTTGCACAAGCAGAAAGTGATGAAATTTTGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATTTAAGTTTTTCAGGACGTAATACACCGCAAAATGAGcc                                                                                                                                      >  2:8805/1‑149 (MQ=255)               tcaaaGCTTGCCGGCTTAATATTATTAATATTTGCACCAGCAGAAAGTGATGAAATTTTGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGaa                                                                                                                                   >  1:303712/1‑149 (MQ=255)                      ttGCCGGCTTAATATTATTAATATTTGCACCAGCAGAAAGTGATGAAATTTTGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACAt                                                                                                                            <  3:131720/149‑1 (MQ=255)                              ttAATATTATTAATATTTGCACCAGCAGAAAGTGATGAAATTTTGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCACAAAATGCGCCGAAACTACATTCAAATgg                                                                                                                    <  3:198126/149‑1 (MQ=255)                                   attattAATATTTGCACCAGCAGAAAGTGATGAAATTTTGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCACAAAATGCGCCGAAACTACATTCAAATGGTAGAt                                                                                                               >  3:81711/1‑149 (MQ=255)                                             tttGCACCAGCAGAAAGTGATGAAATTTTGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGc                                                                                                     >  2:395924/1‑149 (MQ=255)                                                     agcagAAAGTGATGAAATTTTGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTAc                                                                                             <  4:382157/149‑1 (MQ=255)                                                        agAAAGTGATGAAATTTTGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCaa                                                                                          >  4:67674/1‑149 (MQ=255)                                                         gAAAGTGATGAAATTTTGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAAc                                                                                         <  1:350968/149‑1 (MQ=255)                                                                  gaAATTTTGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGAttt                                                                                <  2:134126/149‑1 (MQ=255)                                                                  gaAATTTTGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGAttt                                                                                <  3:248803/149‑1 (MQ=255)                                                                  gaAATTTTGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACAGTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGAttt                                                                                <  2:278264/149‑1 (MQ=255)                                                                      ttttGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTc                                                                            <  4:96093/149‑1 (MQ=255)                                                                         tGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCt                                                                         <  4:311173/149‑1 (MQ=255)                                                                          gTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCtg                                                                        >  3:81714/1‑149 (MQ=255)                                                                               gATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGAc                                                                   >  2:546/1‑149 (MQ=255)                                                                               gATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGAc                                                                   >  2:294185/1‑149 (MQ=255)                                                                                aTAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGACa                                                                  >  4:17055/1‑149 (MQ=255)                                                                                 tAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGACAt                                                                 <  3:127796/149‑1 (MQ=255)                                                                                      agaagaTTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGACATTGCaa                                                            >  1:379800/1‑149 (MQ=255)                                                                                         agaTTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGACATTGCAATaa                                                         <  2:326054/149‑1 (MQ=255)                                                                                          gaTTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCACAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGACATTGCAATAAc                                                        <  4:81711/149‑1 (MQ=255)                                                                                            ttttGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGACATTGCAATAACaa                                                      >  2:19943/1‑149 (MQ=255)                                                                                                   aaaTGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGACATTGCAATAACAATATCTTc                                               <  2:303712/149‑1 (MQ=255)                                                                                                             tAAAACCAGAAAATTTAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGACATTGCAATAACAATATCTTCAGGAGATAtt                                     <  1:8805/149‑1 (MQ=255)                                                                                                                             aaGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGACATTGCAATAACAATATCTTCAGGAGATATTTTTTGACTTTCTTTaa                     <  3:67674/149‑1 (MQ=255)                                                                                                                                tttttCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGACATTGCAATAACAATATCTTCAGGAGATATTTTTTGACTTTCTTTAACaa                  >  4:473969/1‑149 (MQ=255)                                                                                                                                tttttCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGACATTGCAATAACAATATCTTCAGGAGATATTTTTTGACTTTCTTTAACaa                  >  4:370704/1‑149 (MQ=255)                                                                                                                                       ggACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGACATTGCAATAACAATATCTTCAGGAGATATTTTTTGACTTTCTTTAACAAGAttttt           <  1:294185/149‑1 (MQ=255)                                                                                                                                       ggACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGACATTGCAATAACAATATCTTCAGGAGATATTTTTTGACTTTCTTTAACAAGAttttt           <  3:17055/149‑1 (MQ=255)                                                                                                                                           gTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGACATTGCAATAACAATATCTTCAGGAGATATTTTTTGACTTTCTTTAACAAGATTTTTAGGa       >  3:380088/1‑149 (MQ=255)                                                                                                                                            tAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGACGTTGCAATAACAATATCTTCAGGAGATATTTTTTGACTTTCTTTAACAAGATTTTTAGGaa      <  4:81714/149‑1 (MQ=255)                                                                                                                                             aaTACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGACATTGCAATAACAATATCTTCAGGAGATATTTTTTGACTTTCTTTAACAAGATTTTTAGGAAc     >  2:278098/1‑149 (MQ=255)                                                                                                                                               tACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGACATTGCAATAACAATATCTTCAGGAGATATTTTTTGACTTTCTTTAACAAGATTTTTAGGAACaa   >  2:472287/1‑149 (MQ=255)                                                                                                                                                acacCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGACATTGCAATAACAATATCTTCAGGAGATATTTTTTGACTTTCTTTAACAAGATTTTTAGGAACaaa  >  1:199795/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                GTATATTTATCAAATCAAAGCTTGCCGGCTTAATATTATTAATATTTGCACCAGCAGAAAGTGATGAAATTTTGTTTCGATAAAGAGAAGATTTTGTGAAATGAGCAATAAAACCAGAAAATATAAGTTTTTCAGGACGTAATACACCGCAAAATGCGCCGAAACTACATTCAAATGGTAGATGCTGATGTGCGGATTTACCAACTACGGATTTGCTCCCTGATGACATTGCAATAACAATATCTTCAGGAGATATTTTTTGACTTTCTTTAACAAGATTTTTAGGAACAAA  >  NC_000913/4580991‑4581282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑