Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 748,806 A→G 7.1% S59S (TCT→TCC)  ybgO ← putative fimbrial protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913748,8060AG 7.1% 176.4 / 3.8 56S59S (TCT→TCC) ybgOputative fimbrial protein
Reads supporting (aligned to +/- strand):  ref base A (27/25);  new base G (2/2);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01

ATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCAGTTCAGCGCCATTGCAGACTTCAACGGCAGAAATAACAGGCAGA  >  NC_000913/748659‑748951
                                                                                                                                                   |                                                                                                                                                 
aTCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAg                                                                                                                                                  >  2:280136/1‑149 (MQ=255)
 tCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGa                                                                                                                                                 >  2:462438/1‑149 (MQ=255)
  cGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGAt                                                                                                                                                >  4:390179/1‑149 (MQ=255)
       tCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCa                                                                                                                                           <  4:76652/149‑1 (MQ=255)
       tCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCa                                                                                                                                           >  1:40257/1‑149 (MQ=255)
                      gTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCggg                                                                                                                            >  2:405078/1‑149 (MQ=255)
                               aGTTGATAATAACGATCCTGCACCCCCTGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCAtt                                                                                                                   <  3:414595/149‑1 (MQ=255)
                               aGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCAtt                                                                                                                   <  1:276520/149‑1 (MQ=255)
                                    ataataACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATACCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTg                                                                                                              <  1:424820/149‑1 (MQ=255)
                                     taataaCGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTgg                                                                                                             >  2:286511/1‑149 (MQ=255)
                                      aataaCGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGt                                                                                                            >  4:356394/1‑149 (MQ=255)
                                         aaCGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACa                                                                                                         <  3:390179/149‑1 (MQ=255)
                                                  gCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGt                                                                                                <  2:375867/149‑1 (MQ=255)
                                                   cACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGtt                                                                                               <  3:336497/149‑1 (MQ=255)
                                                      ccccGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGGTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGaa                                                                                            >  1:626/1‑149 (MQ=255)
                                                          ggATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGAAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGc                                                                                        <  3:103676/149‑1 (MQ=255)
                                                                        ccAGGCGTAGATATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCGGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTa                                                                          >  3:279430/1‑149 (MQ=255)
                                                                        ccAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCGGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTa                                                                          >  1:342782/1‑149 (MQ=255)
                                                                        ccAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTa                                                                          >  2:289405/1‑149 (MQ=255)
                                                                          aGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACt                                                                        >  4:308655/1‑149 (MQ=255)
                                                                            gCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTgc                                                                      >  3:351267/1‑149 (MQ=255)
                                                                                    aTGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAg                                                              >  4:121486/1‑149 (MQ=255)
                                                                                     tGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGc                                                                                                                      >  2:344299/1‑92 (MQ=255)
                                                                                     tGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGc                                                                                                                      <  1:344299/92‑1 (MQ=255)
                                                                                      gCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGAt                                                            <  1:201917/149‑1 (MQ=255)
                                                                                      gCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGAt                                                            <  1:462438/149‑1 (MQ=255)
                                                                                      gCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGAt                                                            >  3:462156/1‑149 (MQ=255)
                                                                                        tCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGt                                                          >  4:435717/1‑149 (MQ=255)
                                                                                         cGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTa                                                         <  1:274028/149‑1 (MQ=255)
                                                                                                 aaGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATa                                                 <  1:280136/149‑1 (MQ=255)
                                                                                                 aaGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATa                                                 <  4:136473/149‑1 (MQ=255)
                                                                                                  aGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAg                                                >  4:431040/1‑149 (MQ=255)
                                                                                                    ttGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCa                                              <  4:73033/149‑1 (MQ=255)
                                                                                                       ccGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCAGtt                                           <  2:256970/149‑1 (MQ=255)
                                                                                                         gTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCAGTTCa                                         >  2:327551/1‑149 (MQ=255)
                                                                                                         gTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCAGTTCa                                         >  1:242766/1‑149 (MQ=255)
                                                                                                           tGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCAGTTCAgc                                       <  1:289405/149‑1 (MQ=255)
                                                                                                             gTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGAtt                                                                                >  4:464909/1‑106 (MQ=255)
                                                                                                             gTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGAtt                                                                                <  3:464909/106‑1 (MQ=255)
                                                                                                              tATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCAGTTCAGCGcc                                    >  4:426808/1‑149 (MQ=255)
                                                                                                                   ttgtCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCAGTTCAGCGCCATTGc                               <  3:308655/149‑1 (MQ=255)
                                                                                                                      tCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCAtt                                                                                                                   >  3:386647/1‑62 (MQ=255)
                                                                                                                      tCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCAtt                                                                                                                   <  4:386647/62‑1 (MQ=255)
                                                                                                                       cacaATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCAGTTCAGCGCCATTGCAGAc                           >  3:137762/1‑149 (MQ=255)
                                                                                                                        acaATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCAGTTCAGCGCCATTGCAGACt                          >  4:57104/1‑149 (MQ=255)
                                                                                                                               aCGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCAGTTCAGCGCCATTGCAGACTTCAACgg                   >  2:57399/1‑149 (MQ=255)
                                                                                                                                 gggAATTTTAATATCGTCGGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCAGTTCAGCGCCATTGCAGACTTCAACGGCa                 <  2:342782/149‑1 (MQ=255)
                                                                                                                                 gggAATTTTAATATCGTCGGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCAGTTCAGCGCCATTGCAGACTTCAACGGCa                 <  4:279430/149‑1 (MQ=255)
                                                                                                                                 gggAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCAGTTCAGCGCCATTGCAGACTTCAACGGCa                 <  2:40257/149‑1 (MQ=255)
                                                                                                                                  ggAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCAGTTCAGCGCCATTGCAGACTTCAACGGCAg                >  1:163565/1‑149 (MQ=255)
                                                                                                                                    aaTTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCAGTTCAGCGCCATTGCAGACTTCAACGGCAGaa              <  4:462156/149‑1 (MQ=255)
                                                                                                                                      ttttAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCAGTTCAGCGCCATTGCAGACTTCAACGGCAGAAAt            >  1:298641/1‑149 (MQ=255)
                                                                                                                                        ttAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCAGTTCAGCGCCATTGCAGACTTCAACGGCAGAAATaa          <  4:351267/149‑1 (MQ=255)
                                                                                                                                        ttAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCAGTTCAGCGCCAGTGCAGACTTCAACGGCAGAGATaa          <  1:405078/149‑1 (MQ=255)
                                                                                                                                           atatCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCAGTTCAGCGCCATTGCAGACTTCAACGGCAGAAATAACAg       <  2:163565/149‑1 (MQ=255)
                                                                                                                                                gTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATAGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCAGTTCAGCGCCATTGCAGACTTCAACGGCAGAAATAACAGGCAGa  >  4:66515/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACCGGGTTTGGCATTGCCTGGTACAGCAAACGGTTGAATCGCTTCTGATTTTTCTACTGCGCCACCAGATGTACCAAAATAGCAGTTCAGCGCCATTGCAGACTTCAACGGCAGAAATAACAGGCAGA  >  NC_000913/748659‑748951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑