BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,117,057	T→C	T56A (ACC→GCC)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,117,057	0	T	C	98.1%	529.9 / NA	157	T56A (ACC→GCC)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base T (3/0); new base C (65/88); total (68/89)
Fisher's exact test for biased strand distribution p-value = 8.08e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 158 total aligned reads displayed.

AGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACA‑GATATCGCAAGGCAAATGGCGACCAA  >  NC_000913/4116786‑4117327

 ggATCCACTTCAGTTGGGCTGCGGCGAAGTATGAGTCAATCCCCGTACCGGTCTTGCGGCTGAGATAATCTTCTAAACCGGCACGTTGTGAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGTCGCTTTCTTTTTCGCAGACAAGAGTGGTTTCACGCTGGTTCTTCATACCGATAGCTGAAATTTGATCGGAACTGATTTCGGCTTTCGCAAGCACTTCTACCAGCGTGGAGCTTTGGGCGGcc                                                                                                                                                                                                                                                                             <  4:66136/275‑1 (MQ=255)

 ggAGCCACTTCACTTTGGTGCCAGAGAAGTACGAGTCAATCACCAGACCGTTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGcc                                                                                                                                                                                                                                                                             <  2:185090/275‑1 (MQ=255)

      cacctctgtttGGTGGCATAAAAGTCCGGGTTACGCACCAGACCGGTATTGCTGCGGATTTAATCGTCTGAACCGTCACGTTTTAAATGCTCGCAGATTTCGGCAGTACGACGGCACTGGCAGACAATGGCGGTGTGGATAGGCTTGCCGGTTTCTTTTTCCCAGACAAGAGTGGTTTCACGCGGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGAt                                                                                                                                                                                                                                                                        <  4:322863/267‑1 (MQ=255)

       acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGAtt                                                                                                                                                                                                                                                                       >  3:491473/1‑275 (MQ=255)

              tttGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGTTTTCGCCAGCCCTTCTACCAGCGTGGATCTTTGGGCGGACCGGGTTTCCAtt                                                                                                                                                                                                                                                                 >  4:240775/1‑274 (MQ=255)

                 ggTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTggg                                                                                                                                                                                                                                                              <  1:319209/274‑1 (MQ=255)

                  gTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTc                                                                                                                                                                                                                                                            <  2:141308/275‑1 (MQ=255)

                   tGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCCGATTTCCATTGGGt                                                                                                                                                                                                                                                             >  2:119602/1‑273 (MQ=255)

                      cAGAAAACTACGGGTCAAGCACCAGAACGGGATTGCTGCGGATATGAACGTCTAAGCCGTGTCGTTTTAAAAGCTCGCAGATTTCTGCGGTATGAAGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACACTGGTTCGTAATACCGAGAGCTGCAATTTGATCGGAACTGATATCGGGTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGAGTTCCATTGGGTCgtgt                                                                                                                                                                                                                                                        <  3:392257/275‑1 (MQ=255)

                            aGTACGGGTCAATCACCAGACCGGTATTGCTGGGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTAcc                                                                                                                                                                                                                                                  <  2:378860/275‑1 (MQ=255)

                                   gTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTCCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTg                                                                                                                                                                                                                                           >  2:472131/1‑275 (MQ=255)

                                        tCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTg                                                                                                                                                                                                                                                                >  3:255871/1‑249 (MQ=255)

                                        tCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtt                                                                                                                                                                                                                                        <  1:30326/273‑1 (MQ=255)

                                            cAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCAt                                                                                                                                                                                                                                                                  >  2:78257/1‑243 (MQ=255)

                                            cAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTc                                                                                                                                                                                                                                                            <  1:255871/249‑1 (MQ=255)

                                             aGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGCTATCGGCTTTTGCCAGCACTTCTACCAGCGTGGAGTTTTGGGCGGTCCAGCTTTCCATTGGGTCGTGTTCTACCCAACCTGGGTTTGGGtt                                                                                                                                                                                                                                 >  3:82536/1‑274 (MQ=255)

                                                ccGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTggg                                                                                                                                                                                                                                                              <  4:78257/243‑1 (MQ=255)

                                                    tATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCCGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGc                                                                                                                                                                                                                          >  2:304515/1‑275 (MQ=255)

                                                         ctgcGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTg                                                                                                                                                                                                                                                                >  2:227262/1‑232 (MQ=255)

                                                         ctgcGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTTTACCCAAACTGGTTTTGGGTGTATTTGCTCaa                                                                                                                                                                                                                      >  1:274327/1‑274 (MQ=255)

                                                           gcGTATATAAACTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTATGCGGTACGACGGCACTGCCAGACAATGGCGTTATGTATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAAtt                                                                                                                                                                                                                   <  1:286564/275‑1 (MQ=255)

                                                           gcGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGGTATCGGCTTTCGCCAGCACTTCTACAAGGGTGGAGCTTTGGGCGGTCCAGCTTTCCATTGGGTCGTGTTCTACCACACCCGGTTTTGGGTAGATTTGCTCaagt                                                                                                                                                                                                                    >  1:264304/1‑272 (MQ=255)

                                                             ggATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTc                                                                                                                                                                                                                                                            <  4:227262/232‑1 (MQ=255)

                                                              gATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGTCAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCCGACAATAGTGGTTTCACGCTGGTTCGTAGTACCGATAGCTGCAATCTGATCGCAACTGATATCGGCTTTCGCTCTCACTTCTACCACCGTGGCGCTTTGGGTGTCCCAGCTTTCCATTGGGTCGTGTTCTTACCAACCTGGTTCTGGGTAGCTTTGCTCAATTTctg                                                                                                                                                                                                                >  4:451413/1‑273 (MQ=255)

                                                              gATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGATCGTTGGTCGGCCCAGCTTTCCATTGGGGCGGTTGTTTCCCCACCTGGTTTTGGGTAGGTTTGCTCACATTtgc                                                                                                                                                                                                                >  3:437299/1‑275 (MQ=255)

                                                                    agcttctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATAACGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCg                                                                                                                                                                                                          <  2:356297/273‑1 (MQ=255)

                                                                     tattgtAAGCCGGCAAGTTTTAAATGGTCGAGGATTTCTGCGGTAGGAGGGACCCGGCAACAAAGGGCGTTTTAGATAGGCTTGCCGGTTTCTGTTTCGCCAACAATAGTTGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGCTCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                                                                                                                                         <  3:5921/270‑1 (MQ=255)

                                                                      cttctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGac                                                                                                                                                                                                        <  1:491473/275‑1 (MQ=255)

                                                                         ctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATCGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGTACTTATACCAGCGTGGAGCTTTGGGTGGCACGGATTTCCATTGGGTCGGGTTCTACCAAACCTGGATTTGGTGAGCTTTTCACAAATTCGCGCGGCGACACg                                                                                                                                                                                                     >  4:356297/1‑275 (MQ=255)

                                                                             gccGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                                                                                  <  1:82536/273‑1 (MQ=255)

                                                                             cgcggacagTTTTAAATGCTAGACGGTTTCTGCAGTACGACGGCATTCCCAGACAATGGGGTTATAGGTAGGCTTGCCGGTTTCTTTTTCCCAGGAAGTAGGGGTTTCACGCTGGTTCGGAAAACCGATAGCTGCAATTTGATCTGAGCTAATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAGCCTGGTTTTGGGTAGAGTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                                                                                  <  4:331946/266‑1 (MQ=255)

                                                                             cacgttacttgttAAATGGTTGTAGAGCTCTGGCGTACGAAGGCACTGCAAGACAATTGCGTTATAGATAGGCTTTCCGGTTTCTTGTTCCCAGACAATAGTGGTTTCCCGCTGGTTCGTAATTCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                                                                                  <  4:386357/263‑1 (MQ=255)

                                                                              ccGTTACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAAGAGTGGTTTCACGCAGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTTATATCGACTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa                                                                                                                                                                                                 <  1:592659/274‑1 (MQ=255)

                                                                              ccGGCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCAGGTTTCTTTTTCCCAGACAATAGTTGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGACCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa                                                                                                                                                                                                 <  2:105019/274‑1 (MQ=255)

                                                                                 tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCCGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGGTTTGGGTAGATTTGCTCAAATTTGCGCTGCGccaccttcatga                                                                                                                                                                                              >  3:488837/1‑266 (MQ=255)

                                                                                 tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGCTTTCCATTGGGTCGTTTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGacaccgcaatggt                                                                                                                                                                                             >  3:18010/1‑266 (MQ=255)

                                                                                 tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCACCCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGa                                                                                                                                                                                              >  1:350759/1‑274 (MQ=255)

                                                                                   aCGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGc                                                                                                                                                                                                           >  2:60002/1‑259 (MQ=255)

                                                                                      ttttAAATGCTTGTAGATTTCGTGGGTACGACGGCACTGCCATACAATGGCGTTTTGGTTAGGCTTGCCGGTGTCTTTTTCCCTGTCCATAGTGGTTTCACTCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATTTTgg                                                                                                                                                                                        <  4:304515/275‑1 (MQ=255)

                                                                                      ttttAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAGATTCGCGCTGCTACACGCTAATGatatagt                                                                                                                                                                                        >  2:246679/1‑272 (MQ=255)

                                                                                           aggggttgCAGAGTTCTTGGGTCAGCCGGCACTGCCAGGCAATGGGGTTTGCGATGGGCTGTCCTGTTTCTTTTTCCCAGAAACTAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCg                                                                                                                                                                                   <  4:119602/268‑1 (MQ=255)

                                                                                              gCTCGCAGATTTGTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                                                <  1:5492/275‑1 (MQ=255)

                                                                                              gCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                                                <  1:233733/275‑1 (MQ=255)

                                                                                              gCTCCCAGATTTCTGCGGTACGACGGCCCTGCCAGACAATGGCTTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                                                <  2:427832/275‑1 (MQ=255)

                                                                                                 cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATCGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACGCGGTTTTGGGTAGATTTGCTCAAATTTGTGATGCGACACGCTAATGTTTTTGGctgtctcttc                                                                                                                                                                              >  3:593716/1‑265 (MQ=255)

                                                                                                     gATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                                                <  1:212651/268‑1 (MQ=255)

                                                                                                         tttgaggTACGATGGCACTGCCAGACAATGGAGTTATAGTTAAGCTTGCAGGTTTCTTGTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACGTCTGCCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGAc                                                                                                                                                                     <  3:175756/270‑1 (MQ=255)

                                                                                                                  cgacgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCTCCACGCTACTGTTATTGTCGTCGTGATCCATTACGACcacgcg                                                                                                                                                               >  4:26227/1‑272 (MQ=255)

                                                                                                                                     tGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATGGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATCTTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCg                                                                                                                                         >  1:88429/1‑275 (MQ=255)

                                                                                                                                            atagatagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGTCCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCa                                                                                                                                   <  1:458338/274‑1 (MQ=255)

                                                                                                                                            atagatagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCaa                                                                                                                                  <  2:83696/275‑1 (MQ=255)

                                                                                                                                                    gCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCCTTGGGTCGTGTTCTACCCAACCTGGCTTTGGGTAGATTTGCTCAAATTCGAGCTGCGATACGCTCATGATATTGGCATCGTGATCCATTACGACCGCGCGGTCGCTGGTGGTGCCATGGTGGAGCGCCGCGat                                                                                                                              >  2:456553/1‑271 (MQ=255)

                                                                                                                                                       tGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGc                                                                                                                                                                                                           >  1:382537/1‑191 (MQ=255)

                                                                                                                                                       tGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGGGGTGCCCTGGTCTGGCGCAACGATATAttt                                                                                                                        >  3:560071/1‑274 (MQ=255)

                                                                                                                                                        gCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                                                                                  <  2:164934/199‑1 (MQ=255)

                                                                                                                                                        gCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcg                                                                                                                                                                   >  2:425924/1‑230 (MQ=255)

                                                                                                                                                           ggTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                                                                                       <  3:382537/191‑1 (MQ=255)

                                                                                                                                                             tttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCTGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGAt                                                                                                                                                                                                                                                                        <  4:218478/124‑1 (MQ=255)

                                                                                                                                                                cTTTTTCCCAGGCAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTATCGCCAGCACTTCTACCAGCGTGGGGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTc                                                                                                              <  2:110225/275‑1 (MQ=255)

                                                                                                                                                                cTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCTTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTc                                                                                                              <  2:240775/275‑1 (MQ=255)

                                                                                                                                                                  ttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCGAATTCGCGCTGCGCCACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGTGCTGGTGTTGCCCTGGTCTAGCGCACCGATCTATTTTTTTTCAGTctt                                                                                                            >  1:406837/1‑273 (MQ=255)

                                                                                                                                                                  ttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGGTATATTTTTTTTTAGTCAt                                                                                                            >  2:465861/1‑275 (MQ=255)

                                                                                                                                                                        cAGACAAGGGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAAATGATATCGGCTTTTGCCAGCACTTCTACCAGCGTGGAGCTTGGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa                                                                                                      <  1:139195/275‑1 (MQ=255)

                                                                                                                                                                                agggttttagcgcgGGTTCGTACTACCGAGAGCTGAAATTTGATCGGAACTGATATCGGATTTCGCCAGCAGTTCTACCAGCGGGGAGTTTTGGGCGGCCCAGATTTCCATTGGGTTGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCg                                                                                              <  2:254092/265‑1 (MQ=255)

                                                                                                                                                                                 gTGGTTTCACTCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                                                                             <  1:294942/275‑1 (MQ=255)

                                                                                                                                                                                 gTGGTTTCACGCTTGTTCGGAATACCTGTTGCTTCAATTTGATCGGAACTGTTATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                                                                             <  4:472131/275‑1 (MQ=255)

                                                                                                                                                                                 gTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                                                                             <  2:26227/275‑1 (MQ=255)

                                                                                                                                                                                 gTGGTTTCACGCTGGTTCGTAATACCGATAAATGCAATTGGAGCGTAACTGATATCGGCTTACGCCAGCACTTCGACCAGCGTGGAGCTTTGGGCGGCCCAGAGTTCCATTTGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                                                                             <  1:1022/275‑1 (MQ=255)

                                                                                                                                                                                 gTGGTTTCACGCTAGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGAGATCGGCGTTCGCCAGCACTTCTACCAGCGGGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGAAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                                                                             <  2:125548/275‑1 (MQ=255)

                                                                                                                                                                                   ggttcaccGCTGTTTCGGAATCCCGTTAGCTGCAATTTGGTCGGAACTGTTATCGGGTTTCGCCAGCACTTCTACCAGCGTGGGGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                                                                                           <  4:127003/268‑1 (MQ=255)

                                                                                                                                                                                   ggTTTCACGCTGGTTCGTAATTCCATTAGCTGCATTTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGGGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                                                                                           <  1:297272/275‑1 (MQ=255)

                                                                                                                                                                                    gTTTAAAGCTGGTTCATAATACCGATAGCTGGAATTTGATCGGGACTTATATCGTCTTTCGGCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTACATTGGAGCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGAAACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGt                                                                                          <  1:500741/275‑1 (MQ=255)

                                                                                                                                                                                         aCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTTCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCCGGTGGTTCCCT‑GTCGAGCCCCACCATATCTTTTTTTTTTGTCCTGTTTAATTTTACCGTTTTCTTAtt                                                                                    >  4:349777/1‑275 (MQ=255)

                                                                                                                                                                                         aCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGACATATTTTTTTTCAGTCATGTTTAATTGTTCCGTCGTCttat                                                                                     >  2:346735/1‑275 (MQ=255)

                                                                                                                                                                                           gCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtttttttt                                                                                                                   <  2:147559/243‑1 (MQ=255)

                                                                                                                                                                                              ggTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTTCGACACGCTAATGATCTTGGCATCGTTATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATCTTTTTTTTCAGTCATGTTTAATTGTCCCTTAGCCATTTTACAg                                                                                >  2:191889/1‑275 (MQ=255)

                                                                                                                                                                                                ttCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCg                                                                              <  4:11248/275‑1 (MQ=255)

                                                                                                                                                                                                 tagtgacaCCGATACAGGCATTTTGAGCGGAATTGATATCAGCTTTCGCCAGCAGTTCTACTGTCGTGGAGCTTTGGGCGGCCCAGATTGCCATTGTGTCGGGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCg                                                                              <  4:193457/267‑1 (MQ=255)

                                                                                                                                                                                                          cGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACGTGGTGTTGGGTAGATTTGCTCAAATTCGCGATGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGTAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCtttt                                                                      <  2:20540/273‑1 (MQ=255)

                                                                                                                                                                                                            aTAGCTGCAATCTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACTACCGCGCGGGAGCTGGTGGTTCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTATTTGTCCCGTAGTCATATTACACCGAAGCTTTTTGtt                                                                  >  1:177118/1‑275 (MQ=255)

                                                                                                                                                                                                             tAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAcc                                                                                                                                                                                                                                             <  2:270197/103‑1 (MQ=255)

                                                                                                                                                                                                                cTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGa                                                              <  1:396123/275‑1 (MQ=255)

                                                                                                                                                                                                                          gtCGGAACTGATATCGGCTTTCGCAAGCCCTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTtgtg                                                    <  4:66082/274‑1 (MQ=255)

                                                                                                                                                                                                                          aTCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTtgt                                                     <  2:451413/274‑1 (MQ=255)

                                                                                                                                                                                                                               aaCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGtt                                                 <  1:488837/273‑1 (MQ=255)

                                                                                                                                                                                                                                     aatCGTCTTTTGCCAGCACTTCTACCAGCGTGGCGCTTTGGGCGGACCAGCTTTCCATTGGGTCGTGTTCTACTCCACCTGGTTTTGGGTAGATTTGCTCAAATTCGCTCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTttc                                         <  1:566238/274‑1 (MQ=255)

                                                                                                                                                                                                                                         ggCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCCACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTTGTTTCCTTTTCTTcc                                     >  1:569633/1‑275 (MQ=255)

                                                                                                                                                                                                                                             ttCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTc                                                                                                              <  2:96751/198‑1 (MQ=255)

                                                                                                                                                                                                                                              tCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTCCGACCGCGCGGGAGCTGGTTGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGATAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTGTTCCacaac                                >  3:438887/1‑275 (MQ=255)

                                                                                                                                                                                                                                              tCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAATCTTTTTGTTTTGAAGGAGTTGTTGTTTCCtttttcttcacata                                >  3:402191/1‑265 (MQ=255)

                                                                                                                                                                                                                                              gcgcacgtaCTTCTCCCGACGTGGAGCTTTGGTCGGCACAAATTGTCATTGGGTCGTGTTCCACCCAACCTGGTTTTTGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGTTATTGGCATCTTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGTAACGATATATTTGTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCacaac                                <  3:303552/267‑1 (MQ=255)

                                                                                                                                                                                                                                                          cttccAGCGTGGAGCTTTTGGCGGACCAGATTTCCATTGGGTCGGGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACA‑GATATCGc                    <  3:88429/271‑1 (MQ=255)

                                                                                                                                                                                                                                                          cTACCAGCGTGGATCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACA‑GATATCGc                    <  2:342160/274‑1 (MQ=255)

                                                                                                                                                                                                                                                                gCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTAc                                                                                  >  1:100754/1‑207 (MQ=255)

                                                                                                                                                                                                                                                                 cGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTTTCCCGTAGTCATATGACAGCGAGTCTTTTTGTTTTGAAGGAGTTGGGGTTTCCTTTTCTTCCACAA‑ACACGATATCGAAAGGCaaa            >  4:325926/1‑275 (MQ=255)

                                                                                                                                                                                                                                                                  gTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTACTTGTCCCGTAGTCATATTACAGCGAAGCTTTTTTTTTTTAAGGCGTTGTGGTTTCCTTTTCTTCCACAACAAA‑GATATAGCCAGGCaagt           >  3:385821/1‑273 (MQ=255)

                                                                                                                                                                                                                                                                  gTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTGGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAACGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCAGGTTTAATTGTCCCGTAGTCACATTACAGCGAAGCTTTTTGTTTTGAAGGAGTGGTGGTTTCCTTTTCTTCCCCAACACA‑TATATCCAAAGGCaaag           >  3:344574/1‑274 (MQ=255)

                                                                                                                                                                                                                                                                    ggAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCg                                                                              <  3:100754/207‑1 (MQ=255)

                                                                                                                                                                                                                                                                           tGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTATGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATCTTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTAATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCTACAACACA‑GATATCGCAAGGCAAATGGCGCCCaa  >  2:324323/1‑275 (MQ=255)

                                                                                                                                                                                                                                                                           ggggCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGTTGTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACA‑GATATCGCAAGGCAAATGGCGACCaa  <  3:413431/274‑1 (MQ=255)

AGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACA‑GATATCGCAAGGCAAATGGCGACCAA  >  NC_000913/4116786‑4117327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`