Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,117,057 T→C T56A (ACC→GCC)  glpK ← glycerol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,117,0570TC98.1% 529.9 / NA 157T56A (ACC→GCC) glpKglycerol kinase
Reads supporting (aligned to +/- strand):  ref base T (3/0);  new base C (65/88);  total (68/89)
Fisher's exact test for biased strand distribution p-value = 8.08e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 158 total aligned reads displayed.

AGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAA  >  NC_000913/4116786‑4117327
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                               
 ggATCCACTTCAGTTGGGCTGCGGCGAAGTATGAGTCAATCCCCGTACCGGTCTTGCGGCTGAGATAATCTTCTAAACCGGCACGTTGTGAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGTCGCTTTCTTTTTCGCAGACAAGAGTGGTTTCACGCTGGTTCTTCATACCGATAGCTGAAATTTGATCGGAACTGATTTCGGCTTTCGCAAGCACTTCTACCAGCGTGGAGCTTTGGGCGGcc                                                                                                                                                                                                                                                                             <  4:66136/275‑1 (MQ=255)
 ggAGCCACTTCACTTTGGTGCCAGAGAAGTACGAGTCAATCACCAGACCGTTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGcc                                                                                                                                                                                                                                                                             <  2:185090/275‑1 (MQ=255)
      cacctctgtttGGTGGCATAAAAGTCCGGGTTACGCACCAGACCGGTATTGCTGCGGATTTAATCGTCTGAACCGTCACGTTTTAAATGCTCGCAGATTTCGGCAGTACGACGGCACTGGCAGACAATGGCGGTGTGGATAGGCTTGCCGGTTTCTTTTTCCCAGACAAGAGTGGTTTCACGCGGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGAt                                                                                                                                                                                                                                                                        <  4:322863/267‑1 (MQ=255)
       acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGAtt                                                                                                                                                                                                                                                                       >  3:491473/1‑275 (MQ=255)
              tttGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGTTTTCGCCAGCCCTTCTACCAGCGTGGATCTTTGGGCGGACCGGGTTTCCAtt                                                                                                                                                                                                                                                                 >  4:240775/1‑274 (MQ=255)
                 ggTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTggg                                                                                                                                                                                                                                                              <  1:319209/274‑1 (MQ=255)
                  gTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTc                                                                                                                                                                                                                                                            <  2:141308/275‑1 (MQ=255)
                   tGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCCGATTTCCATTGGGt                                                                                                                                                                                                                                                             >  2:119602/1‑273 (MQ=255)
                      cAGAAAACTACGGGTCAAGCACCAGAACGGGATTGCTGCGGATATGAACGTCTAAGCCGTGTCGTTTTAAAAGCTCGCAGATTTCTGCGGTATGAAGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACACTGGTTCGTAATACCGAGAGCTGCAATTTGATCGGAACTGATATCGGGTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGAGTTCCATTGGGTCgtgt                                                                                                                                                                                                                                                        <  3:392257/275‑1 (MQ=255)
                            aGTACGGGTCAATCACCAGACCGGTATTGCTGGGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTAcc                                                                                                                                                                                                                                                  <  2:378860/275‑1 (MQ=255)
                                   gTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTCCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTg                                                                                                                                                                                                                                           >  2:472131/1‑275 (MQ=255)
                                        tCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTg                                                                                                                                                                                                                                                                >  3:255871/1‑249 (MQ=255)
                                        tCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtt                                                                                                                                                                                                                                        <  1:30326/273‑1 (MQ=255)
                                            cAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCAt                                                                                                                                                                                                                                                                  >  2:78257/1‑243 (MQ=255)
                                            cAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTc                                                                                                                                                                                                                                                            <  1:255871/249‑1 (MQ=255)
                                             aGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGCTATCGGCTTTTGCCAGCACTTCTACCAGCGTGGAGTTTTGGGCGGTCCAGCTTTCCATTGGGTCGTGTTCTACCCAACCTGGGTTTGGGtt                                                                                                                                                                                                                                 >  3:82536/1‑274 (MQ=255)
                                                ccGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTggg                                                                                                                                                                                                                                                              <  4:78257/243‑1 (MQ=255)
                                                    tATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCCGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGc                                                                                                                                                                                                                          >  2:304515/1‑275 (MQ=255)
                                                         ctgcGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTg                                                                                                                                                                                                                                                                >  2:227262/1‑232 (MQ=255)
                                                         ctgcGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTTTACCCAAACTGGTTTTGGGTGTATTTGCTCaa                                                                                                                                                                                                                      >  1:274327/1‑274 (MQ=255)
                                                           gcGTATATAAACTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTATGCGGTACGACGGCACTGCCAGACAATGGCGTTATGTATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAAtt                                                                                                                                                                                                                   <  1:286564/275‑1 (MQ=255)
                                                           gcGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGGTATCGGCTTTCGCCAGCACTTCTACAAGGGTGGAGCTTTGGGCGGTCCAGCTTTCCATTGGGTCGTGTTCTACCACACCCGGTTTTGGGTAGATTTGCTCaagt                                                                                                                                                                                                                    >  1:264304/1‑272 (MQ=255)
                                                             ggATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTc                                                                                                                                                                                                                                                            <  4:227262/232‑1 (MQ=255)
                                                              gATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGTCAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCCGACAATAGTGGTTTCACGCTGGTTCGTAGTACCGATAGCTGCAATCTGATCGCAACTGATATCGGCTTTCGCTCTCACTTCTACCACCGTGGCGCTTTGGGTGTCCCAGCTTTCCATTGGGTCGTGTTCTTACCAACCTGGTTCTGGGTAGCTTTGCTCAATTTctg                                                                                                                                                                                                                >  4:451413/1‑273 (MQ=255)
                                                              gATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGATCGTTGGTCGGCCCAGCTTTCCATTGGGGCGGTTGTTTCCCCACCTGGTTTTGGGTAGGTTTGCTCACATTtgc                                                                                                                                                                                                                >  3:437299/1‑275 (MQ=255)
                                                                    agcttctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATAACGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCg                                                                                                                                                                                                          <  2:356297/273‑1 (MQ=255)
                                                                     tattgtAAGCCGGCAAGTTTTAAATGGTCGAGGATTTCTGCGGTAGGAGGGACCCGGCAACAAAGGGCGTTTTAGATAGGCTTGCCGGTTTCTGTTTCGCCAACAATAGTTGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGCTCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                                                                                                                                         <  3:5921/270‑1 (MQ=255)
                                                                      cttctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGac                                                                                                                                                                                                        <  1:491473/275‑1 (MQ=255)
                                                                         ctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATCGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGTACTTATACCAGCGTGGAGCTTTGGGTGGCACGGATTTCCATTGGGTCGGGTTCTACCAAACCTGGATTTGGTGAGCTTTTCACAAATTCGCGCGGCGACACg                                                                                                                                                                                                     >  4:356297/1‑275 (MQ=255)
                                                                             gccGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                                                                                  <  1:82536/273‑1 (MQ=255)
                                                                             cgcggacagTTTTAAATGCTAGACGGTTTCTGCAGTACGACGGCATTCCCAGACAATGGGGTTATAGGTAGGCTTGCCGGTTTCTTTTTCCCAGGAAGTAGGGGTTTCACGCTGGTTCGGAAAACCGATAGCTGCAATTTGATCTGAGCTAATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAGCCTGGTTTTGGGTAGAGTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                                                                                  <  4:331946/266‑1 (MQ=255)
                                                                             cacgttacttgttAAATGGTTGTAGAGCTCTGGCGTACGAAGGCACTGCAAGACAATTGCGTTATAGATAGGCTTTCCGGTTTCTTGTTCCCAGACAATAGTGGTTTCCCGCTGGTTCGTAATTCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                                                                                  <  4:386357/263‑1 (MQ=255)
                                                                              ccGTTACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAAGAGTGGTTTCACGCAGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTTATATCGACTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa                                                                                                                                                                                                 <  1:592659/274‑1 (MQ=255)
                                                                              ccGGCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCAGGTTTCTTTTTCCCAGACAATAGTTGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGACCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa                                                                                                                                                                                                 <  2:105019/274‑1 (MQ=255)
                                                                                 tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCCGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGGTTTGGGTAGATTTGCTCAAATTTGCGCTGCGccaccttcatga                                                                                                                                                                                              >  3:488837/1‑266 (MQ=255)
                                                                                 tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGCTTTCCATTGGGTCGTTTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGacaccgcaatggt                                                                                                                                                                                             >  3:18010/1‑266 (MQ=255)
                                                                                 tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCACCCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGa                                                                                                                                                                                              >  1:350759/1‑274 (MQ=255)
                                                                                   aCGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGc                                                                                                                                                                                                           >  2:60002/1‑259 (MQ=255)
                                                                                      ttttAAATGCTTGTAGATTTCGTGGGTACGACGGCACTGCCATACAATGGCGTTTTGGTTAGGCTTGCCGGTGTCTTTTTCCCTGTCCATAGTGGTTTCACTCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATTTTgg                                                                                                                                                                                        <  4:304515/275‑1 (MQ=255)
                                                                                      ttttAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAGATTCGCGCTGCTACACGCTAATGatatagt                                                                                                                                                                                        >  2:246679/1‑272 (MQ=255)
                                                                                           aggggttgCAGAGTTCTTGGGTCAGCCGGCACTGCCAGGCAATGGGGTTTGCGATGGGCTGTCCTGTTTCTTTTTCCCAGAAACTAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCg                                                                                                                                                                                   <  4:119602/268‑1 (MQ=255)
                                                                                              gCTCGCAGATTTGTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                                                <  1:5492/275‑1 (MQ=255)
                                                                                              gCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                                                <  1:233733/275‑1 (MQ=255)
                                                                                              gCTCCCAGATTTCTGCGGTACGACGGCCCTGCCAGACAATGGCTTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                                                <  2:427832/275‑1 (MQ=255)
                                                                                                 cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATCGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACGCGGTTTTGGGTAGATTTGCTCAAATTTGTGATGCGACACGCTAATGTTTTTGGctgtctcttc                                                                                                                                                                              >  3:593716/1‑265 (MQ=255)
                                                                                                     gATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                                                <  1:212651/268‑1 (MQ=255)
                                                                                                         tttgaggTACGATGGCACTGCCAGACAATGGAGTTATAGTTAAGCTTGCAGGTTTCTTGTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACGTCTGCCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGAc                                                                                                                                                                     <  3:175756/270‑1 (MQ=255)
                                                                                                                  cgacgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCTCCACGCTACTGTTATTGTCGTCGTGATCCATTACGACcacgcg                                                                                                                                                               >  4:26227/1‑272 (MQ=255)
                                                                                                                                     tGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATGGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATCTTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCg                                                                                                                                         >  1:88429/1‑275 (MQ=255)
                                                                                                                                            atagatagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGTCCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCa                                                                                                                                   <  1:458338/274‑1 (MQ=255)
                                                                                                                                            atagatagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCaa                                                                                                                                  <  2:83696/275‑1 (MQ=255)
                                                                                                                                                    gCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCCTTGGGTCGTGTTCTACCCAACCTGGCTTTGGGTAGATTTGCTCAAATTCGAGCTGCGATACGCTCATGATATTGGCATCGTGATCCATTACGACCGCGCGGTCGCTGGTGGTGCCATGGTGGAGCGCCGCGat                                                                                                                              >  2:456553/1‑271 (MQ=255)
                                                                                                                                                       tGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGc                                                                                                                                                                                                           >  1:382537/1‑191 (MQ=255)
                                                                                                                                                       tGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGGGGTGCCCTGGTCTGGCGCAACGATATAttt                                                                                                                        >  3:560071/1‑274 (MQ=255)
                                                                                                                                                        gCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                                                                                  <  2:164934/199‑1 (MQ=255)
                                                                                                                                                        gCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcg                                                                                                                                                                   >  2:425924/1‑230 (MQ=255)
                                                                                                                                                           ggTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                                                                                       <  3:382537/191‑1 (MQ=255)
                                                                                                                                                             tttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCTGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGAt                                                                                                                                                                                                                                                                        <  4:218478/124‑1 (MQ=255)
                                                                                                                                                                cTTTTTCCCAGGCAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTATCGCCAGCACTTCTACCAGCGTGGGGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTc                                                                                                              <  2:110225/275‑1 (MQ=255)
                                                                                                                                                                cTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCTTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTc                                                                                                              <  2:240775/275‑1 (MQ=255)
                                                                                                                                                                  ttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCGAATTCGCGCTGCGCCACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGTGCTGGTGTTGCCCTGGTCTAGCGCACCGATCTATTTTTTTTCAGTctt                                                                                                            >  1:406837/1‑273 (MQ=255)
                                                                                                                                                                  ttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGGTATATTTTTTTTTAGTCAt                                                                                                            >  2:465861/1‑275 (MQ=255)
                                                                                                                                                                        cAGACAAGGGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAAATGATATCGGCTTTTGCCAGCACTTCTACCAGCGTGGAGCTTGGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa                                                                                                      <  1:139195/275‑1 (MQ=255)
                                                                                                                                                                                agggttttagcgcgGGTTCGTACTACCGAGAGCTGAAATTTGATCGGAACTGATATCGGATTTCGCCAGCAGTTCTACCAGCGGGGAGTTTTGGGCGGCCCAGATTTCCATTGGGTTGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCg                                                                                              <  2:254092/265‑1 (MQ=255)
                                                                                                                                                                                 gTGGTTTCACTCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                                                                             <  1:294942/275‑1 (MQ=255)
                                                                                                                                                                                 gTGGTTTCACGCTTGTTCGGAATACCTGTTGCTTCAATTTGATCGGAACTGTTATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                                                                             <  4:472131/275‑1 (MQ=255)
                                                                                                                                                                                 gTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                                                                             <  2:26227/275‑1 (MQ=255)
                                                                                                                                                                                 gTGGTTTCACGCTGGTTCGTAATACCGATAAATGCAATTGGAGCGTAACTGATATCGGCTTACGCCAGCACTTCGACCAGCGTGGAGCTTTGGGCGGCCCAGAGTTCCATTTGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                                                                             <  1:1022/275‑1 (MQ=255)
                                                                                                                                                                                 gTGGTTTCACGCTAGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGAGATCGGCGTTCGCCAGCACTTCTACCAGCGGGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGAAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                                                                             <  2:125548/275‑1 (MQ=255)
                                                                                                                                                                                   ggttcaccGCTGTTTCGGAATCCCGTTAGCTGCAATTTGGTCGGAACTGTTATCGGGTTTCGCCAGCACTTCTACCAGCGTGGGGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                                                                                           <  4:127003/268‑1 (MQ=255)
                                                                                                                                                                                   ggTTTCACGCTGGTTCGTAATTCCATTAGCTGCATTTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGGGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                                                                                           <  1:297272/275‑1 (MQ=255)
                                                                                                                                                                                    gTTTAAAGCTGGTTCATAATACCGATAGCTGGAATTTGATCGGGACTTATATCGTCTTTCGGCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTACATTGGAGCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGAAACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGt                                                                                          <  1:500741/275‑1 (MQ=255)
                                                                                                                                                                                         aCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTTCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCCGGTGGTTCCCTGTCGAGCCCCACCATATCTTTTTTTTTTGTCCTGTTTAATTTTACCGTTTTCTTAtt                                                                                    >  4:349777/1‑275 (MQ=255)
                                                                                                                                                                                         aCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGACATATTTTTTTTCAGTCATGTTTAATTGTTCCGTCGTCttat                                                                                     >  2:346735/1‑275 (MQ=255)
                                                                                                                                                                                           gCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtttttttt                                                                                                                   <  2:147559/243‑1 (MQ=255)
                                                                                                                                                                                              ggTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTTCGACACGCTAATGATCTTGGCATCGTTATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATCTTTTTTTTCAGTCATGTTTAATTGTCCCTTAGCCATTTTACAg                                                                                >  2:191889/1‑275 (MQ=255)
                                                                                                                                                                                                ttCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCg                                                                              <  4:11248/275‑1 (MQ=255)
                                                                                                                                                                                                 tagtgacaCCGATACAGGCATTTTGAGCGGAATTGATATCAGCTTTCGCCAGCAGTTCTACTGTCGTGGAGCTTTGGGCGGCCCAGATTGCCATTGTGTCGGGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCg                                                                              <  4:193457/267‑1 (MQ=255)
                                                                                                                                                                                                          cGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACGTGGTGTTGGGTAGATTTGCTCAAATTCGCGATGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGTAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCtttt                                                                      <  2:20540/273‑1 (MQ=255)
                                                                                                                                                                                                            aTAGCTGCAATCTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACTACCGCGCGGGAGCTGGTGGTTCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTATTTGTCCCGTAGTCATATTACACCGAAGCTTTTTGtt                                                                  >  1:177118/1‑275 (MQ=255)
                                                                                                                                                                                                             tAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAcc                                                                                                                                                                                                                                             <  2:270197/103‑1 (MQ=255)
                                                                                                                                                                                                                cTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGa                                                              <  1:396123/275‑1 (MQ=255)
                                                                                                                                                                                                                          gtCGGAACTGATATCGGCTTTCGCAAGCCCTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTtgtg                                                    <  4:66082/274‑1 (MQ=255)
                                                                                                                                                                                                                          aTCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTtgt                                                     <  2:451413/274‑1 (MQ=255)
                                                                                                                                                                                                                               aaCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGtt                                                 <  1:488837/273‑1 (MQ=255)
                                                                                                                                                                                                                                     aatCGTCTTTTGCCAGCACTTCTACCAGCGTGGCGCTTTGGGCGGACCAGCTTTCCATTGGGTCGTGTTCTACTCCACCTGGTTTTGGGTAGATTTGCTCAAATTCGCTCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTttc                                         <  1:566238/274‑1 (MQ=255)
                                                                                                                                                                                                                                         ggCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCCACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTTGTTTCCTTTTCTTcc                                     >  1:569633/1‑275 (MQ=255)
                                                                                                                                                                                                                                             ttCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTc                                                                                                              <  2:96751/198‑1 (MQ=255)
                                                                                                                                                                                                                                              tCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTCCGACCGCGCGGGAGCTGGTTGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGATAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTGTTCCacaac                                >  3:438887/1‑275 (MQ=255)
                                                                                                                                                                                                                                              tCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAATCTTTTTGTTTTGAAGGAGTTGTTGTTTCCtttttcttcacata                                >  3:402191/1‑265 (MQ=255)
                                                                                                                                                                                                                                              gcgcacgtaCTTCTCCCGACGTGGAGCTTTGGTCGGCACAAATTGTCATTGGGTCGTGTTCCACCCAACCTGGTTTTTGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGTTATTGGCATCTTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGTAACGATATATTTGTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCacaac                                <  3:303552/267‑1 (MQ=255)
                                                                                                                                                                                                                                                          cttccAGCGTGGAGCTTTTGGCGGACCAGATTTCCATTGGGTCGGGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGc                    <  3:88429/271‑1 (MQ=255)
                                                                                                                                                                                                                                                          cTACCAGCGTGGATCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGc                    <  2:342160/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                gCGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTAc                                                                                  >  1:100754/1‑207 (MQ=255)
                                                                                                                                                                                                                                                                 cGTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTTTCCCGTAGTCATATGACAGCGAGTCTTTTTGTTTTGAAGGAGTTGGGGTTTCCTTTTCTTCCACAAACACGATATCGAAAGGCaaa            >  4:325926/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                  gTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTACTTGTCCCGTAGTCATATTACAGCGAAGCTTTTTTTTTTTAAGGCGTTGTGGTTTCCTTTTCTTCCACAACAAAGATATAGCCAGGCaagt           >  3:385821/1‑273 (MQ=255)
                                                                                                                                                                                                                                                                  gTGGAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTGGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAACGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCAGGTTTAATTGTCCCGTAGTCACATTACAGCGAAGCTTTTTGTTTTGAAGGAGTGGTGGTTTCCTTTTCTTCCCCAACACATATATCCAAAGGCaaag           >  3:344574/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                    ggAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCg                                                                              <  3:100754/207‑1 (MQ=255)
                                                                                                                                                                                                                                                                           tGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTATGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATCTTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTAATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCTACAACACAGATATCGCAAGGCAAATGGCGCCCaa  >  2:324323/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                           ggggCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGTTGTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCaa  <  3:413431/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                               
AGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAA  >  NC_000913/4116786‑4117327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: