Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,955,176 Δ1 bp 14.7% coding (1832/2430 nt) torZ ← trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,955,1760T.14.7% 102.2 / 12.4 34coding (1832/2430 nt)torZtrimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base T (15/14);  new base . (3/2);  total (18/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

TTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCATAGTGACGCGTTGCGCACGGGCACCTTTCTGAGCGGCATCATAGAAAAATTTCAGCCACGCCATTTCATCTTTACCTTC  >  NC_000913/1955028‑1955324
                                                                                                                                                     |                                                                                                                                                    
ttCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACt                                                                                                                                                      >  1:160254/1‑149 (MQ=255)
     caGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACTgttc                                                                                                                                                  <  3:329410/148‑1 (MQ=255)
       ggAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACTgttcgtt                                                                                                                                               >  2:381930/1‑149 (MQ=255)
        gAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACTgttcgtt                                                                                                                                               <  4:372187/148‑1 (MQ=255)
              gccagGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACTGTTCGTTCTtctc                                                                                                                                         >  3:367973/1‑148 (MQ=255)
                  ggTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACTGTTCGTTCTTCTCGCtgt                                                                                                                                    <  1:381930/149‑1 (MQ=255)
                             ccGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACTGTTCGTTCTTCTCGCTGTGGCGCATTTc                                                                                                                          <  3:369468/148‑1 (MQ=255)
                                   aaTCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAg                                                                                                                    <  4:176617/148‑1 (MQ=255)
                                     tCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCTTAACGAAC‑GTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGttt                                                                                                                 >  2:335949/1‑149 (MQ=255)
                                              ccAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTtgctg                                                                                                        >  1:257019/1‑149 (MQ=255)
                                              ccAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTtgctg                                                                                                        >  2:211906/1‑149 (MQ=255)
                                               cAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTtgctg                                                                                                        >  3:226229/1‑148 (MQ=255)
                                               cAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTtgctg                                                                                                        >  3:144788/1‑148 (MQ=255)
                                                    ttttCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGa                                                                                                   <  4:75788/148‑1 (MQ=255)
                                                        ccAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGc                                                                                               <  4:196682/148‑1 (MQ=255)
                                                           aCGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCAtt                                                                                            >  3:122076/1‑148 (MQ=255)
                                                           aCGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCAGAACGAACAGTAc‑gttcgttatg                                                                                                                                            <  4:239345/100‑4 (MQ=255)
                                                           aCGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCAGAACGAACAGTAc‑gttcgttatg                                                                                                                                            >  3:239345/1‑97 (MQ=255)
                                                                                         ttGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACTGTTCGTTCTTCTCTCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCATAGTGACGCGTTGCGCACgg                                                             <  1:407322/149‑1 (MQ=255)
                                                                                                aCCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCATAGTGACGCGTTGCGCACGGGCACCt                                                       >  4:122314/1‑148 (MQ=255)
                                                                                                  ccAGCGCATTTTTCTCCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGCTCAGTTTATTTTGCTGCCAGCAGGCATTAAACATTGGCATAGTGACGCGTTCCGCACGGGCACCTTTc                                                    >  2:15761/1‑149 (MQ=255)
                                                                                                  ccAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCATAGTGACGCGTTGCGCACGGGCACCTTTc                                                    <  2:160254/149‑1 (MQ=255)
                                                                                                           ttttCACCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCATAGTGACGCGTTGCGCACGGGCACCTTTCTGAGCGGc                                            >  3:195596/1‑148 (MQ=255)
                                                                                                                      tCGGCGCGGAAATCACCATAACGAAC‑GTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCATAGTGACGCGTTGCGCACGGGCACCTTTCTGAGCGGCATCATAGaaaa                                 <  4:195596/148‑1 (MQ=255)
                                                                                                                         gcgcGGGAATAACCATAGCGAAC‑CTACTGTTGGTTATTCTCGCTGCGGCGCATTTCGATCTGCTGCTTTTGATGCCGGAAGCCATTAAGCATTTGCATAGTGAGGCGTTGCGCACGGGCACCGTTATGAGCGGCATCATAGAAAAAttt                             >  2:386455/1‑149 (MQ=255)
                                                                                                                           gcgGAAATCACCATAACGAAC‑GTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCATAGTGACGCGTTGCGCACGGGCACCTTTCTGAGCGGCATCATAGAAAAATTTc                            <  4:226229/148‑1 (MQ=255)
                                                                                                                            cgGAAATCACCTTAACGAAC‑GTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCATAGTGACGCGTTGCGCACGGGCACCTTTCTGAGCGGCATCATAGAAAAATTTCa                           <  3:235030/148‑1 (MQ=255)
                                                                                                                            cgGAAATCACCATAACGAAC‑GTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCATAGTGACGCGTTGCGCACGGGCACCTTTCTGAGCGGCATCATAGAAAAATTTCa                           >  4:33690/1‑148 (MQ=255)
                                                                                                                             ggAAATCACCATAACGAAC‑GTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCATAGTGACGCGTTGCGCACGGGCACCTTTCTGAGCGGCATCATAGAAAAATTTCAGc                         >  1:34402/1‑149 (MQ=255)
                                                                                                                                        gaacgaacaGTAC‑GTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCATAGTGACGCGTTGCGCACGGGCa                                                          <  2:145802/104‑1 (MQ=255)
                                                                                                                                        gaacgaacaGTAC‑GTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCATAGTGACGCGTTGCGCACGGGCa                                                          >  1:145802/2‑105 (MQ=255)
                                                                                                                                        gaacgaacaGTAC‑GTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCATAGTGACGCGTTGCGCACGGGCACCTTTCTGAGCGGCATCATAGAAAAATTTCAGCCACGCCATTTc              >  1:328415/2‑149 (MQ=255)
                                                                                                                                         aacgaacaGTAC‑GTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCATAGTGACGCGTTGCGCACGGGCACCTTTc                                                    >  1:39874/1‑110 (MQ=255)
                                                                                                                                         aacgaacaGTAC‑GTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCATAGTGACGCGTTGCGCACGGGCACCTTTc                                                    <  2:39874/110‑1 (MQ=255)
                                                                                                                                          acgaac‑gTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCATAGTGACGCGTTGCGCACGGGCACCTTTCTGAGCGGCATCATAGAAAAATTTCAGCCACGCCATTTCa             <  3:122314/148‑1 (MQ=255)
                                                                                                                                            gaac‑gTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCATAGTGACGCGTTGCGCACGGGCACCTTTCTGAGCGGCATCATAGAAAAATTTCAGCCACGCCATTTCATCt          >  2:186728/1‑149 (MQ=255)
                                                                                                                                              ac‑gTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCATAGTGACGCGTTGCGCACGGGCACCTTTCTGAGCGGCATCATAGAAAAATTTCAGCCACGCCATTTCATCttt        <  1:186728/149‑1 (MQ=255)
                                                                                                                                                     tGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCATAGTGACGCGTTGCGCACGGGCACCTTTCTGAGCGGCATCATAGAAAAATTTCAGCCACGCCATTTCATCTTTACCTTc  >  2:55156/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAAC‑GTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCATAGTGACGCGTTGCGCACGGGCACCTTTCTGAGCGGCATCATAGAAAAATTTCAGCCACGCCATTTCATCTTTACCTTC  >  NC_000913/1955028‑1955324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑