Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,440,866 A→G 8.7% L247P (CTG→CCG) ‡ fabB ← 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,440,8660AG 8.7% 134.1 / 3.7 46L247P (CTG→CCG) ‡fabB3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
Reads supporting (aligned to +/- strand):  ref base A (20/22);  new base G (2/2);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

TTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAG  >  NC_000913/2440719‑2441013
                                                                                                                                                   |                                                                                                                                                   
ttCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCa                                                                                                                                                     >  3:344572/1‑148 (MQ=255)
     gTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCgt                                                                                                                                               >  2:38453/1‑149 (MQ=255)
        aTCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCTGGCGCgtggt                                                                                                                                            <  2:68552/149‑3 (MQ=255)
        aTCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGtt                                                                                                                                            >  2:55267/1‑149 (MQ=255)
        aTCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGAGAGCACCACGCGCCAGCGCgtgt                                                                                                                                             >  4:281952/1‑148 (MQ=255)
         tCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGtt                                                                                                                                            >  3:4095/1‑148 (MQ=255)
          cGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTc                                                                                                                                           <  3:187937/148‑1 (MQ=255)
                 ggTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCtt                                                                                                                                   <  2:162130/149‑1 (MQ=255)
                    aTCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCa                                                                                                                                 >  4:128690/1‑148 (MQ=255)
                      cAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGct                                                                                                                                      >  4:181496/1‑141 (MQ=255)
                      cAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGct                                                                                                                                      <  3:181496/141‑1 (MQ=255)
                       aaCGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCa                                                                                                                                         >  2:164441/1‑137 (MQ=255)
                       aaCGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCa                                                                                                                                         <  1:164441/137‑1 (MQ=255)
                       aaCGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCa                                                                                                                             <  1:399500/149‑1 (MQ=255)
                        aCGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCa                                                                                                                             >  3:146611/1‑148 (MQ=255)
                        aCGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCAc                                                                                                                            >  2:53495/1‑149 (MQ=255)
                             aTGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGGGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCa                                                                                                                             >  4:266576/1‑143 (MQ=255)
                             aTGCATCGCCATCTTCATGCAGCGTACTGCGCCTGCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGTTCTTCAACCa                                                                                                                             <  3:266576/143‑1 (MQ=255)
                                 aTCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGGCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCATTACCATAcc                                                                                                                   <  2:250433/149‑1 (MQ=255)
                                 aTCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATAcc                                                                                                                   <  1:20661/149‑1 (MQ=255)
                                  tCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGctct                                                                                                                                    <  2:51645/131‑1 (MQ=255)
                                  tCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGctct                                                                                                                                    >  1:51645/1‑131 (MQ=255)
                                                        tGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGaaa                                                                                             <  4:146611/148‑1 (MQ=255)
                                                               tCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCAc                                                                                      >  4:129221/1‑148 (MQ=255)
                                                                   cAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGa                                                                                 <  1:55267/149‑1 (MQ=255)
                                                                            cAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTGCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCTATAACGAAACCGTCACGGGGAGCGTCGTaa                                                                        <  2:75478/149‑1 (MQ=255)
                                                                            cAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTaa                                                                        <  1:53495/149‑1 (MQ=255)
                                                                                      tGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAg                                                               <  3:364912/148‑1 (MQ=255)
                                                                                             tCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATAccgccgccg                                                                                                            <  3:73703/96‑1 (MQ=255)
                                                                                             tCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATAccgccgccg                                                                                                            >  4:73703/1‑96 (MQ=255)
                                                                                              cAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGctct                                                                                                                                    >  4:65066/1‑71 (MQ=255)
                                                                                              cAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGctct                                                                                                                                    <  3:65066/71‑1 (MQ=255)
                                                                                                 agGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCgg                                                    <  4:4095/148‑1 (MQ=255)
                                                                                                         ccGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGt                                            <  3:128690/148‑1 (MQ=255)
                                                                                                               ccAACGATTTCAGCATAGATGTGAGCACCCCGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAACCCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTAtt                                      >  4:195219/1‑148 (MQ=255)
                                                                                                                 aaCGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTtag                                   <  1:38453/149‑1 (MQ=255)
                                                                                                                 aaCGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTtag                                   >  1:162591/1‑149 (MQ=255)
                                                                                                                  aCGNTTTCAGCATAGATGTGAGCACCACGCGCCAGCGCATGTTCCAGCTCTTCAAGCACTAACNTACCGCCGCAGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGAGAGGCTTTTCCCGGGGAGTCGTAGTATTtag                                   >  3:250611/1‑148 (MQ=255)
                                                                                                                   cGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTtagta                                 <  1:280598/149‑1 (MQ=255)
                                                                                                                    gATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTtagtag                                >  1:245470/1‑149 (MQ=255)
                                                                                                                                  tgtgAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCAt                   <  4:351097/148‑1 (MQ=255)
                                                                                                                                   gtgAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCAtt                  >  3:398813/1‑148 (MQ=255)
                                                                                                                                       gCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTc             <  1:308746/149‑1 (MQ=255)
                                                                                                                                        gaacacGCGCTGGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTc             >  4:178852/4‑148 (MQ=255)
                                                                                                                                          acacGCGCTGGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGcgtc                                                                            <  2:160256/82‑1 (MQ=255)
                                                                                                                                          acacGCGCTGGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGcgtc                                                                            >  1:160256/2‑83 (MQ=255)
                                                                                                                                                  cAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAg  >  1:154687/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAG  >  NC_000913/2440719‑2441013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑