| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,536,373 | C→G | 16.0% | intergenic (+30/+13) | crr → / ← pdxK | glucose‑specific enzyme IIA component of PTS/pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,536,373 | 0 | C | G | 16.0% | 66.5 / 7.8 | 25 | intergenic (+30/+13) | crr/pdxK | glucose‑specific enzyme IIA component of PTS/pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase |
| Reads supporting (aligned to +/- strand): ref base C (12/9); new base G (2/2); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.11e-01 | |||||||||||
TCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACT > NC_000913/2536233‑2536517 | tCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCGATGGGCGCCAt < 2:365176/149‑1 (MQ=255) aaCATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCa > 1:366947/1‑149 (MQ=255) aTGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCaaa < 4:9402/148‑1 (MQ=255) gAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAAtt > 1:400194/1‑149 (MQ=255) tCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCA‑TTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATc > 4:356661/1‑148 (MQ=255) aaaGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGc > 1:31553/1‑149 (MQ=255) aaaGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGc > 2:83991/1‑149 (MQ=255) aaGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGc > 4:64950/1‑148 (MQ=255) aGAACTGATCAAACTGTCCGGTAGCGTACCCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCGTTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGct > 3:346517/1‑148 (MQ=255) aCTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCtctc > 3:120384/1‑148 (MQ=255) gATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGCTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCGATGGGCGCCAtt < 4:74441/100‑1 (MQ=255) gATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGCTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCGATGGGCGCCAtt > 3:74441/1‑100 (MQ=255) aCTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTgg < 2:347112/149‑1 (MQ=255) gTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTgg < 4:66452/141‑1 (MQ=255) gTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTgg > 3:66452/1‑141 (MQ=255) tCCGCATCAACAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTATCGCATCACTTCCAGTACGCGCAACCCCGCTc < 4:346517/148‑1 (MQ=255) aagTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCa > 3:268653/1‑148 (MQ=255) taaaaaaaTGGCGCCGATGGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCt > 2:315853/4‑149 (MQ=255) aaaaaTGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCttt < 3:64950/148‑1 (MQ=255) aaaaaTGGCGCCCATCGGCGCCA‑TTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTc < 3:356661/148‑1 (MQ=255) aaaaTGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCa < 2:366947/149‑1 (MQ=255) aaaaTGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCa < 2:400194/149‑1 (MQ=255) cccATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCa > 3:20228/1‑148 (MQ=255) cccATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCAc > 1:104162/1‑149 (MQ=255) ccATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACt < 1:83991/149‑1 (MQ=255) | TCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACT > NC_000913/2536233‑2536517 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |