Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 207,486 G→T 12.5% M787I (ATG→ATT)  dnaE → DNA polymerase III alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913207,4860GT12.5% 116.0 / 8.5 40M787I (ATG→ATT) dnaEDNA polymerase III alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (18/17);  new base T (3/2);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.90e-01

TGGCGATGAAAATCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTCCACGTCAACGACGACGGCGAAATCGTGTATGGTA  >  NC_000913/207344‑207628
                                                                                                                                              |                                                                                                                                              
tGGCGATGAAAATCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGAccgc                                                                                                                                           <  4:385373/148‑1 (MQ=255)
  gCGATGAAAATCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTCATTAccgccg                                                                                                                                         <  4:230814/148‑1 (MQ=255)
      tGAAAATCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGatat                                                                                                                                     <  4:122182/148‑1 (MQ=255)
        aaaaTCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCCCTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCCGTAATGACCGCTGATATgg                                                                                                                                   <  4:67720/148‑1 (MQ=255)
             cTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGacaac                                                                                                                              >  4:394489/1‑148 (MQ=255)
             cTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGacaac                                                                                                                              >  3:160533/1‑148 (MQ=255)
              ttCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAacac                                                                                                                            >  1:88171/1‑149 (MQ=255)
               tCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTCATTAccgccgc                                                                                                                                        <  4:63387/136‑2 (MQ=255)
               tCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTCATTAccgccgc                                                                                                                                        >  3:63387/1‑135 (MQ=255)
                         ggAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGa                                                                                                                                        <  4:279363/126‑1 (MQ=255)
                         ggAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGa                                                                                                                                        >  3:279363/1‑126 (MQ=255)
                                       gTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGa                                                                                                    <  3:329311/148‑1 (MQ=255)
                                         tACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGCTAATGACCGCCGATATGGACAACACCGAGAAGATGGTGGGTCTGGTGGATg                                                                                                  <  4:343349/148‑1 (MQ=255)
                                               tttAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCt                                                                                            >  4:14915/1‑148 (MQ=255)
                                                ttAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTg                                                                                           >  4:378795/1‑148 (MQ=255)
                                                      aaTCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGAt                                                                                     >  4:334196/1‑148 (MQ=255)
                                                       aTCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATgg                                                                                   >  1:103487/1‑149 (MQ=255)
                                                        ttgCACTGTGCGGTCTATGCCGTGGTGTCATATGAAACGTTCTGGCTGAAAGCGCACTATCCTGCGTAGTTTATGGCGGGGGTAATGACCGTGGATATGGTGAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATggg                                                                                  <  2:52068/147‑1 (MQ=255)
                                                         cGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATgggg                                                                                 <  2:98929/149‑1 (MQ=255)
                                                                          gCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGccac                                                                 >  3:310512/1‑148 (MQ=255)
                                                                           cTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGccacc                                                                >  3:368266/1‑148 (MQ=255)
                                                                             ttGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAg                                                              <  4:310512/148‑1 (MQ=255)
                                                                             ttGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGa                                                             <  2:408028/149‑1 (MQ=255)
                                                                              tGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGat                                                            <  2:88171/149‑1 (MQ=255)
                                                                                     atatCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGACGGTGGTGGGTCtgg                                                                                                        >  2:302384/1‑98 (MQ=255)
                                                                                     atatCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGAGGTGGGTCtgg                                                                                                        <  1:302384/98‑1 (MQ=255)
                                                                                      tatCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACt                                                     >  4:206958/1‑148 (MQ=255)
                                                                                      tatCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTGTATGCAGGCGGTAATGACCGCCGATATGGACTACAACGAGGAGGTGGGGGGTCCGGTGGACAAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTc                                                    >  2:212692/1‑149 (MQ=255)
                                                                                          aaaCGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGt                                                >  1:345421/1‑149 (MQ=255)
                                                                                           aaCGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTc                                               >  1:16926/1‑149 (MQ=255)
                                                                                           aaCGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTc                                               <  2:124735/149‑1 (MQ=255)
                                                                                            aCGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTc                                               >  4:259283/1‑148 (MQ=255)
                                                                                                     cTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCAttt                                     <  2:4622/149‑1 (MQ=255)
                                                                                                        aaaGCGCACTATCCTGCGGAGTTTATGGCGCGGTTATTGCCCGCGGTATTGGCCAACCCGGAGAAGGGGTTGGGCTGGGTGGTTGGGTGTTGGCGGTTGGGGCTGAAAATCTGGCCACCAGATATAAACTCCGGTCTTTACCATTTCCa                                  >  2:195151/1‑149 (MQ=255)
                                                                                                                              ttATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTCCACGTCAACGACGACGGCGAAATc            <  2:345421/149‑1 (MQ=255)
                                                                                                                                aTGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTCCACGTCAACGACGACGGCGAAATCg           <  4:160533/148‑1 (MQ=255)
                                                                                                                                 tatcggcggTCATTACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTCCACGTCAACGACGACGGCGAAATCgtg         >  1:121377/4‑149 (MQ=255)
                                                                                                                                    cggcggTCATTACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAACCCTGCCACCAGTTATAAACTCCGGTCTTTACCATTTCCACGTCAACGACGACGGCGAAATCGTGTa       >  3:285267/1‑148 (MQ=255)
                                                                                                                                     ggcggTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTCCACGTCAACGACGACGGCGAAATCGTGTAt      <  3:334196/148‑1 (MQ=255)
                                                                                                                                        ggTGATGGCCGCCGATATGGACAAAAACGATAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATACTGCCACCAGATATAAACTCCGGTCTTTACCATTTCCACGTCAACGACGACGGCGAAATCGTGTATGGTa  >  2:282819/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                              
TGGCGATGAAAATCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTCCACGTCAACGACGACGGCGAAATCGTGTATGGTA  >  NC_000913/207344‑207628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑