| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,550,576 | A→T | 16.7% | intergenic (‑31/+65) | yfeX ← / ← yfeY | porphyrinogen oxidase, cytoplasmic/RpoE‑regulated lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,550,576 | 0 | A | T | 16.7% | 96.7 / 12.8 | 36 | intergenic (‑31/+65) | yfeX/yfeY | porphyrinogen oxidase, cytoplasmic/RpoE‑regulated lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (15/15); new base T (3/3); total (18/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
TTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAGGACCACG > NC_000913/2550442‑2550718 | ttACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCAttgt < 3:269054/148‑1 (MQ=255)ttACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGATTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGGATTGCGTGGCattatt < 2:352215/149‑1 (MQ=255) cacgcaGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATc > 4:207320/1‑127 (MQ=255) cacgcaGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATc < 3:207320/127‑1 (MQ=255) tCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGttt > 4:303700/1‑148 (MQ=255) ttCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTcgc > 2:400716/1‑149 (MQ=255) cTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCgcggcg > 4:250909/1‑148 (MQ=255) ttGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAg < 3:59367/113‑1 (MQ=255) ttGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAg > 4:59367/1‑113 (MQ=255) ttGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTg > 3:4651/1‑148 (MQ=255) tGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGt < 3:303700/148‑1 (MQ=255) ttCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAg < 3:250909/148‑1 (MQ=255) aTCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCa > 1:106920/1‑149 (MQ=255) ccAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCaa < 3:181714/148‑1 (MQ=255) aTCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTaa < 1:194940/149‑1 (MQ=255) aaaaTGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAAc < 1:135544/126‑1 (MQ=255) aaaaTGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAAc > 2:135544/1‑126 (MQ=255) cACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGGATTGCGTGGCa < 3:176753/59‑1 (MQ=37) cACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGGATTGCGTGGCa > 4:176753/1‑59 (MQ=37) tctGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGa < 2:4190/137‑1 (MQ=255) tctGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGa > 1:4190/1‑137 (MQ=255) aCCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAgg < 2:106920/149‑1 (MQ=255) aTTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTcc > 2:24555/1‑149 (MQ=255) gTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGaa < 2:14116/149‑1 (MQ=255) ttCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGaa < 4:245566/148‑1 (MQ=255) ttCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAg > 1:331682/1‑149 (MQ=255) cctGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCAtt < 1:407902/149‑1 (MQ=255) ctGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTa > 2:336502/1‑149 (MQ=255) gAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTaa < 4:70076/148‑1 (MQ=255) tcaacaatGCCACGGATTGCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACGGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCtt > 1:146701/6‑149 (MQ=255) acaatGCCACGGATTGCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAAcc < 3:292474/141‑1 (MQ=255) acaatGCCACGGATTGCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAAcc > 4:292474/3‑143 (MQ=255) taatGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAgg > 2:364790/1‑149 (MQ=255) ccACGCAATCCGTAGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAGGACCAc < 2:241731/149‑1 (MQ=255) cACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAGGACCAc > 3:12143/1‑148 (MQ=255) aCGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAGGACCACg > 3:110730/1‑148 (MQ=255) | TTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAGGACCACG > NC_000913/2550442‑2550718 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |