Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,629,495 A→T 12.8% L15Q (CTG→CAG)  rbbA ← ribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,629,4950AT12.8% 137.3 / 11.9 47L15Q (CTG→CAG) rbbAribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein
Reads supporting (aligned to +/- strand):  ref base A (26/15);  new base T (3/3);  total (29/18)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-01

ATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATGCTGCT  >  NC_000913/3629348‑3629641
                                                                                                                                                    |                                                                                                                                                  
aTGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAg                                                                                                                                                   >  1:174767/1‑149 (MQ=255)
  gACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCATG‑CCCGCCAGt                                                                                                                                                  >  3:174835/1‑148 (MQ=255)
    cgcgGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTg                                                                                                                                                >  4:85952/1‑148 (MQ=255)
     gcgGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCTATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTgcg                                                                                                                                              >  2:240444/1‑149 (MQ=255)
                 aaTCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCTATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGcggga                                                                                                                                  <  1:240444/149‑1 (MQ=255)
                    cAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCTGTTGCGCAACTgg                                                                                                                                       >  4:48081/1‑141 (MQ=255)
                    cAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCTGTTGCGCAACTgg                                                                                                                                       <  3:48081/141‑1 (MQ=255)
                    cAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGcgggacgg                                                                                                                               <  1:399761/149‑1 (MQ=255)
                    cAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGcgggacg                                                                                                                                >  3:300934/1‑148 (MQ=255)
                     aaCGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGcgggacggg                                                                                                                              <  1:98422/149‑1 (MQ=255)
                     aaCGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGcgggacggg                                                                                                                              >  1:227762/1‑149 (MQ=255)
                        gACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGaa                                                                                                                            >  4:169661/1‑148 (MQ=255)
                            acaaGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAg                                                                                                                        >  4:131245/1‑148 (MQ=255)
                            acaaGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGt                                                                                                                       >  2:201103/1‑149 (MQ=255)
                             caaGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGtt                                                                                                                      <  1:122395/149‑1 (MQ=255)
                              aaGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGtt                                                                                                                      >  3:370719/1‑148 (MQ=255)
                               aGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTc                                                                                                                     <  3:169661/148‑1 (MQ=255)
                                           cGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCAt                                                                                                         >  3:302593/1‑148 (MQ=255)
                                               gCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTcc                                                                                                                    >  1:119271/1‑133 (MQ=255)
                                               gCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTcc                                                                                                                    <  2:119271/133‑1 (MQ=255)
                                                ccGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCgg                                                                                                    >  3:78908/1‑148 (MQ=255)
                                                   tCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCaa                                                                                                 <  4:302593/148‑1 (MQ=255)
                                                          cAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGTTCCCGTCCCGCCTGTCGCGCAACTGGcgg                                                                                                                                    <  2:378376/107‑1 (MQ=25)
                                                          cAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGTTCCCGTCCCGCCTGTCGCGCAACTGGcgg                                                                                                                                    >  1:378376/1‑107 (MQ=25)
                                                              cAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAg                                                                                     <  2:227762/149‑1 (MQ=255)
                                                                                 gCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAg                                                                   <  4:300934/148‑1 (MQ=255)
                                                                                   cGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttc                                                                >  2:253564/1‑149 (MQ=255)
                                                                                           cGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCCCGGAAGTTCttcattc                                                         >  4:103612/1‑148 (MQ=255)
                                                                                                gTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCg                                                    >  3:70122/1‑148 (MQ=255)
                                                                                                 tGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGc                                                   >  3:277390/1‑148 (MQ=255)
                                                                                                     atTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAccc                                               >  3:120822/1‑148 (MQ=255)
                                                                                                      ttGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCAc                                             >  2:300149/1‑149 (MQ=255)
                                                                                                        gTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGc                                           <  1:109977/149‑1 (MQ=255)
                                                                                                            aGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACg                                       >  2:385263/1‑149 (MQ=255)
                                                                                                               gcAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGccc                                    <  2:174767/149‑1 (MQ=255)
                                                                                                               gcAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGcc                                     <  3:131245/148‑1 (MQ=255)
                                                                                                                          tCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGt                         >  1:322851/1‑149 (MQ=255)
                                                                                                                             aTAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGttt                       <  4:78097/148‑1 (MQ=255)
                                                                                                                              tAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGtttt                      >  3:49124/1‑148 (MQ=255)
                                                                                                                               aaTGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGa                    <  2:228707/149‑1 (MQ=255)
                                                                                                                                 tGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACa                  >  1:254121/1‑149 (MQ=255)
                                                                                                                                          aCG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAg          <  4:120822/148‑1 (MQ=255)
                                                                                                                                                cgccTGTCGCGCAACTGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACTACGAGGTCGTCAGGCCACGGAAGttct                                                               <  3:27478/90‑1 (MQ=255)
                                                                                                                                                cgccTGTCGCGCAACTGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttct                                                               >  4:27478/1‑90 (MQ=255)
                                                                                                                                                 gccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGAtgctg    >  3:137633/1‑148 (MQ=255)
                                                                                                                                                  ccAGTTGCGCGACAGGCGTGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATgctgct  >  2:405871/1‑149 (MQ=255)
                                                                                                                                                   cAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATgctgct  >  3:343063/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATGCTGCT  >  NC_000913/3629348‑3629641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑