Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,629,498	T→C	8.9%	Q14R (CAA→CGA)	rbbA ←	ribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,629,498	0	T	C	8.9%	130.1 / 4.2	45	Q14R (CAA→CGA)	rbbA	ribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein
Reads supporting (aligned to +/- strand):  ref base T (25/16);  new base C (2/2);  total (27/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.81e-01

CGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATGCTGCT  >  NC_000913/3629352‑3629641                                                                                                                                                    |                                                                                                                                                cgcgGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTg                                                                                                                                                >  4:85952/1‑148 (MQ=255)  gcgGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCTATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTgcg                                                                                                                                              >  2:240444/1‑149 (MQ=255)              aaTCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCTATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGcggga                                                                                                                                  <  1:240444/149‑1 (MQ=255)                 cAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCTGTTGCGCAACTgg                                                                                                                                       <  3:48081/141‑1 (MQ=255)                 cAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCTGTTGCGCAACTgg                                                                                                                                       >  4:48081/1‑141 (MQ=255)                 cAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGcgggacgg                                                                                                                               <  1:399761/149‑1 (MQ=255)                 cAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGcgggacg                                                                                                                                >  3:300934/1‑148 (MQ=255)                  aaCGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGcgggacggg                                                                                                                              <  1:98422/149‑1 (MQ=255)                  aaCGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGcgggacggg                                                                                                                              >  1:227762/1‑149 (MQ=255)                     gACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGaa                                                                                                                            >  4:169661/1‑148 (MQ=255)                         acaaGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAg                                                                                                                        >  4:131245/1‑148 (MQ=255)                         acaaGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGt                                                                                                                       >  2:201103/1‑149 (MQ=255)                          caaGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGtt                                                                                                                      <  1:122395/149‑1 (MQ=255)                           aaGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGtt                                                                                                                      >  3:370719/1‑148 (MQ=255)                            aGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTc                                                                                                                     <  3:169661/148‑1 (MQ=255)                                        cGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCAt                                                                                                         >  3:302593/1‑148 (MQ=255)                                            gCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTcc                                                                                                                    >  1:119271/1‑133 (MQ=255)                                            gCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTcc                                                                                                                    <  2:119271/133‑1 (MQ=255)                                             ccGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCgg                                                                                                    >  3:78908/1‑148 (MQ=255)                                                tCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCaa                                                                                                 <  4:302593/148‑1 (MQ=255)                                                       cAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGTTCCCGTCCCGCCTGTCGCGCAACTGGcgg                                                                                                                                    <  2:378376/107‑1 (MQ=25)                                                       cAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGTTCCCGTCCCGCCTGTCGCGCAACTGGcgg                                                                                                                                    >  1:378376/1‑107 (MQ=25)                                                           cAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAg                                                                                     <  2:227762/149‑1 (MQ=255)                                                                              gCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAg                                                                   <  4:300934/148‑1 (MQ=255)                                                                                cGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttc                                                                >  2:253564/1‑149 (MQ=255)                                                                                        cGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCCCGGAAGTTCttcattc                                                         >  4:103612/1‑148 (MQ=255)                                                                                             gTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCg                                                    >  3:70122/1‑148 (MQ=255)                                                                                              tGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGc                                                   >  3:277390/1‑148 (MQ=255)                                                                                                  atTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAccc                                               >  3:120822/1‑148 (MQ=255)                                                                                                   ttGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCAc                                             >  2:300149/1‑149 (MQ=255)                                                                                                     gTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGc                                           <  1:109977/149‑1 (MQ=255)                                                                                                         aGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACg                                       >  2:385263/1‑149 (MQ=255)                                                                                                            gcAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGccc                                    <  2:174767/149‑1 (MQ=255)                                                                                                            gcAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGcc                                     <  3:131245/148‑1 (MQ=255)                                                                                                                       tCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGt                         >  1:322851/1‑149 (MQ=255)                                                                                                                          aTAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGttt                       <  4:78097/148‑1 (MQ=255)                                                                                                                           tAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGtttt                      >  3:49124/1‑148 (MQ=255)                                                                                                                            aaTGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGa                    <  2:228707/149‑1 (MQ=255)                                                                                                                              tGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACa                  >  1:254121/1‑149 (MQ=255)                                                                                                                                       aCG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAg          <  4:120822/148‑1 (MQ=255)                                                                                                                                             cgccTGTCGCGCAACTGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACTACGAGGTCGTCAGGCCACGGAAGttct                                                               <  3:27478/90‑1 (MQ=255)                                                                                                                                             cgccTGTCGCGCAACTGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttct                                                               >  4:27478/1‑90 (MQ=255)                                                                                                                                              gccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGAtgctg    >  3:137633/1‑148 (MQ=255)                                                                                                                                               ccAGTTGCGCGACAGGCGTGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATgctgct  >  2:405871/1‑149 (MQ=255)                                                                                                                                                cAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATgctgct  >  3:343063/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                CGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATGCTGCT  >  NC_000913/3629352‑3629641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑