Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,629,503 G→A 13.8% V12V (GTC→GTT)  rbbA ← ribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,629,5030GA13.8% 114.4 / 7.4 43V12V (GTC→GTT) rbbAribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein
Reads supporting (aligned to +/- strand):  ref base G (22/15);  new base A (3/3);  total (25/18)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATGCTGCT  >  NC_000913/3629365‑3629641
                                                                                                                                           |                                                                                                                                          
aaTCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCTATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGcggga                                                                                                                                  <  1:240444/149‑1 (MQ=255)
   cAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCTGTTGCGCAACTgg                                                                                                                                       >  4:48081/1‑141 (MQ=255)
   cAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCTGTTGCGCAACTgg                                                                                                                                       <  3:48081/141‑1 (MQ=255)
   cAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGcgggacgg                                                                                                                               <  1:399761/149‑1 (MQ=255)
   cAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGcgggacg                                                                                                                                >  3:300934/1‑148 (MQ=255)
    aaCGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGcgggacggg                                                                                                                              >  1:227762/1‑149 (MQ=255)
    aaCGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGcgggacggg                                                                                                                              <  1:98422/149‑1 (MQ=255)
       gACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGaa                                                                                                                            >  4:169661/1‑148 (MQ=255)
           acaaGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAg                                                                                                                        >  4:131245/1‑148 (MQ=255)
           acaaGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGt                                                                                                                       >  2:201103/1‑149 (MQ=255)
            caaGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGtt                                                                                                                      <  1:122395/149‑1 (MQ=255)
             aaGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGtt                                                                                                                      >  3:370719/1‑148 (MQ=255)
              aGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTc                                                                                                                     <  3:169661/148‑1 (MQ=255)
                          cGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCAt                                                                                                         >  3:302593/1‑148 (MQ=255)
                              gCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTcc                                                                                                                    >  1:119271/1‑133 (MQ=255)
                              gCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTcc                                                                                                                    <  2:119271/133‑1 (MQ=255)
                               ccGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCgg                                                                                                    >  3:78908/1‑148 (MQ=255)
                                  tCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCaa                                                                                                 <  4:302593/148‑1 (MQ=255)
                                         cAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGTTCCCGTCCCGCCTGTCGCGCAACTGGcgg                                                                                                                                    <  2:378376/107‑1 (MQ=25)
                                         cAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGTTCCCGTCCCGCCTGTCGCGCAACTGGcgg                                                                                                                                    >  1:378376/1‑107 (MQ=25)
                                             cAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAg                                                                                     <  2:227762/149‑1 (MQ=255)
                                                                gCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAg                                                                   <  4:300934/148‑1 (MQ=255)
                                                                  cGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttc                                                                >  2:253564/1‑149 (MQ=255)
                                                                          cGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCCCGGAAGTTCttcattc                                                         >  4:103612/1‑148 (MQ=255)
                                                                               gTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCg                                                    >  3:70122/1‑148 (MQ=255)
                                                                                tGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGc                                                   >  3:277390/1‑148 (MQ=255)
                                                                                    atTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAccc                                               >  3:120822/1‑148 (MQ=255)
                                                                                     ttGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCAc                                             >  2:300149/1‑149 (MQ=255)
                                                                                       gTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGc                                           <  1:109977/149‑1 (MQ=255)
                                                                                           aGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACg                                       >  2:385263/1‑149 (MQ=255)
                                                                                              gcAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGccc                                    <  2:174767/149‑1 (MQ=255)
                                                                                              gcAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGcc                                     <  3:131245/148‑1 (MQ=255)
                                                                                                         tCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGt                         >  1:322851/1‑149 (MQ=255)
                                                                                                            aTAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGttt                       <  4:78097/148‑1 (MQ=255)
                                                                                                             tAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGtttt                      >  3:49124/1‑148 (MQ=255)
                                                                                                              aaTGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGa                    <  2:228707/149‑1 (MQ=255)
                                                                                                                tGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACa                  >  1:254121/1‑149 (MQ=255)
                                                                                                                         aCG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAg          <  4:120822/148‑1 (MQ=255)
                                                                                                                               cgccTGTCGCGCAACTGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACTACGAGGTCGTCAGGCCACGGAAGttct                                                               <  3:27478/90‑1 (MQ=255)
                                                                                                                               cgccTGTCGCGCAACTGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttct                                                               >  4:27478/1‑90 (MQ=255)
                                                                                                                                gccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGAtgctg    >  3:137633/1‑148 (MQ=255)
                                                                                                                                 ccAGTTGCGCGACAGGCGTGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATgctgct  >  2:405871/1‑149 (MQ=255)
                                                                                                                                  cAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATgctgct  >  3:343063/1‑148 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATGCTGCT  >  NC_000913/3629365‑3629641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑