Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,642,358 T→A 11.4% intergenic (+27/+22) dtpB → / ← rsmJ dipeptide and tripeptide permease B/16S rRNA m(2)G1516 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,642,3580TA11.4% 107.1 / 6.2 35intergenic (+27/+22)dtpB/rsmJdipeptide and tripeptide permease B/16S rRNA m(2)G1516 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (15/16);  new base A (2/2);  total (17/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.31e-01

GCCCGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTCGCCAGC  >  NC_000913/3642214‑3642506
                                                                                                                                                |                                                                                                                                                    
gCCCGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATc                                                                                                                                                  <  1:97077/149‑1 (MQ=255)
      cTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTAc                                                                                                                                             >  4:377883/1‑148 (MQ=255)
        ataCCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGATAACGCTACCCt                                                                                                                                          <  1:7071/149‑1 (MQ=255)
         taCCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCt                                                                                                                                          >  3:262078/1‑148 (MQ=255)
           ccAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAACAGGGTAGCGATAACGCTACCCTgc                                                                                                                                        <  4:26947/148‑2 (MQ=255)
           ccAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGAGGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgtt                                                                                                                                       <  2:26377/149‑1 (MQ=255)
            cAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgtt                                                                                                                                       <  3:308676/148‑1 (MQ=255)
              aCGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgtttta                                                                                                                                    <  2:194117/149‑1 (MQ=255)
                gtgtTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttttag                                                                                                                                   <  4:211145/148‑1 (MQ=255)
                  gtTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttttagtt                                                                                                                                 >  3:290237/1‑148 (MQ=255)
                    ttGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttttagtttt                                                                                                                               <  3:122451/148‑1 (MQ=255)
                     tGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTCGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttttag                                                                                                                                   <  2:315402/143‑1 (MQ=255)
                     tGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttttag                                                                                                                                   >  1:315402/1‑143 (MQ=255)
                       gTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTacac                                                                                                                           >  2:111021/1‑149 (MQ=255)
                                 tctggtcACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCt                                                                                                                 <  2:183137/149‑1 (MQ=255)
                                                  ttGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGGAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGc                                                                                                 >  4:291225/1‑148 (MQ=255)
                                                         agtGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGccc                                                                                          >  4:34868/1‑148 (MQ=255)
                                                           tGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCttt                                                                                       >  2:254172/1‑149 (MQ=255)
                                                                           gCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGtt                                                                        <  4:380496/148‑1 (MQ=255)
                                                                                  cTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTtggcgtg                                                                 <  3:397256/148‑1 (MQ=255)
                                                                                       aCGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACa                                                           >  1:329333/1‑149 (MQ=255)
                                                                                                      gCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTgg                                             <  4:262078/148‑1 (MQ=255)
                                                                                                           aaaGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGt                                        <  3:199108/148‑1 (MQ=255)
                                                                                                            aaGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAg                                      >  1:227244/1‑149 (MQ=255)
                                                                                                                  ttaattaTTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGgcgc                                          >  1:115914/1‑139 (MQ=255)
                                                                                                                  ttaattaTTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGgcgc                                          <  2:115914/139‑1 (MQ=255)
                                                                                                                  ttaattaTTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGAGGCGCGTAGTCCgg                                 >  3:45211/1‑148 (MQ=255)
                                                                                                                         ttCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTa                         >  1:350888/1‑149 (MQ=255)
                                                                                                                          tCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTac                        <  2:227244/149‑1 (MQ=255)
                                                                                                                             tGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTacca                      >  4:386400/1‑148 (MQ=255)
                                                                                                                                      cAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGtt             <  3:377883/148‑1 (MQ=255)
                                                                                                                                       aGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGttt            <  4:154049/148‑1 (MQ=255)
                                                                                                                                       aGGGTAGCGATAACGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGttt            >  3:189335/1‑148 (MQ=255)
                                                                                                                                        gggTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTg           >  3:214930/1‑148 (MQ=255)
                                                                                                                                          gTAGCGATAACGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTc        >  2:367808/1‑149 (MQ=255)
                                                                                                                                                ttATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTCGCCAGc  >  2:396009/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GCCCGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTCGCCAGC  >  NC_000913/3642214‑3642506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑