Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,754,927 A→C 6.7% intergenic (‑499/+46) yiaW ← / ← aldB DUF3302 family inner membrane protein/aldehyde dehydrogenase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,754,9270AC 6.7% 440.5 / 18.7 127intergenic (‑499/+46)yiaW/aldBDUF3302 family inner membrane protein/aldehyde dehydrogenase B
Reads supporting (aligned to +/- strand):  ref base A (50/68);  new base C (4/5);  total (54/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

CGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGA  >  NC_000913/3754781‑3755074
                                                                                                                                                  |                                                                                                                                                   
cGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGc                                                                                                                                                   <  2:375268/149‑1 (MQ=255)
   aCTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCTTACGCGt                                                                                                                                                 <  3:376441/148‑1 (MQ=255)
   aCTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTc                                                                                                                                                <  2:21257/149‑1 (MQ=255)
    cTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCTTACGCGTc                                                                                                                                                <  4:37086/148‑1 (MQ=255)
     ttCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGc                                                                                                                                              >  1:213699/1‑149 (MQ=255)
         gTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAGACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATc                                                                                                                                           <  3:150182/148‑1 (MQ=255)
         gTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATc                                                                                                                                           >  4:61412/1‑148 (MQ=255)
           tGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATGCGACGCTTACGCGTCTTATCatg                                                                                                                                        <  1:328710/149‑3 (MQ=38)
               gTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAAt                                                                                                                                    <  1:330211/149‑1 (MQ=255)
               gTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAAt                                                                                                                                    <  2:6546/149‑1 (MQ=255)
               gTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAAt                                                                                                                                    <  1:181846/149‑1 (MQ=255)
                tAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATGCGACGCTTACGCGTctta                                                                                                                                             >  3:235145/1‑136 (MQ=38)
                    cTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATa                                                                                                                               <  2:196150/149‑1 (MQ=255)
                     tGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATa                                                                                                                               <  4:232733/148‑1 (MQ=255)
                     tGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATa                                                                                                                               <  4:312271/148‑1 (MQ=255)
                     tGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATAc                                                                                                                              >  2:277080/1‑149 (MQ=255)
                     tGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGAGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATa                                                                                                                               <  3:382160/148‑1 (MQ=255)
                                 cACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCa                                                                                                                                      <  3:113505/129‑1 (MQ=255)
                                 cACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCa                                                                                                                                      >  4:113505/1‑129 (MQ=255)
                                  aCGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCa                                                                                                                  <  4:259250/148‑1 (MQ=255)
                                      aGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGcttc                                                                                                             <  2:30196/149‑1 (MQ=255)
                                      aGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGctt                                                                                                              <  3:61412/148‑1 (MQ=255)
                                      aGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGctt                                                                                                              <  4:186432/148‑1 (MQ=255)
                                         tttAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGt                                                                                                                                                 >  4:268098/1‑110 (MQ=255)
                                         tttAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGt                                                                                                                                                 <  3:268098/110‑1 (MQ=255)
                                         tttAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGTCAATGAATACCCAATGCGACCAGCTTCTta                                                                                                          <  1:326141/149‑1 (MQ=255)
                                         tttAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGcttctt                                                                                                           <  4:371992/148‑1 (MQ=255)
                                         tttAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTta                                                                                                          >  2:133819/1‑149 (MQ=255)
                                          ttAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTtat                                                                                                         <  2:21187/149‑1 (MQ=255)
                                          ttAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTta                                                                                                          >  4:130899/1‑148 (MQ=255)
                                            aaaaCGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTatat                                                                                                       <  1:326729/149‑1 (MQ=255)
                                            aaaaCGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTatat                                                                                                       <  2:302394/149‑1 (MQ=255)
                                             aaaCGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTatat                                                                                                       <  4:221134/148‑1 (MQ=255)
                                             aaaCGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTatat                                                                                                       >  4:137876/1‑148 (MQ=255)
                                                  tAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCAAGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGaa                                                                                                  >  4:229395/1‑148 (MQ=255)
                                                      ttGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATGCGACGCTTACGCGTCTTATCat                                                                                                                                         >  1:161032/1‑104 (MQ=25)
                                                        gTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGcccc                                                                                           >  1:352630/1‑149 (MQ=255)
                                                           acaacGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAAc                                                                                        <  2:348626/149‑1 (MQ=255)
                                                            caacGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAAc                                                                                        >  3:203424/1‑148 (MQ=255)
                                                             aacGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACgg                                                                                      >  2:114779/1‑149 (MQ=255)
                                                              acGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGt                                                                                     <  1:66171/149‑1 (MQ=255)
                                                                gTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGttt                                                                                   <  2:213699/149‑1 (MQ=255)
                                                                 tGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGttt                                                                                   <  3:26743/148‑1 (MQ=255)
                                                                  gcgcTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTAt                                                                                 <  1:144067/149‑1 (MQ=255)
                                                                    gcTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATcc                                                                               <  2:77227/149‑1 (MQ=255)
                                                                      tttGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGa                                                                             >  2:115267/1‑149 (MQ=255)
                                                                      tttGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGa                                                                             >  2:406318/1‑149 (MQ=255)
                                                                      tttGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGa                                                                             <  1:266645/149‑1 (MQ=255)
                                                                       ttGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGa                                                                             >  3:336683/1‑148 (MQ=255)
                                                                       ttGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGa                                                                             >  3:371482/1‑148 (MQ=255)
                                                                       ttGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGa                                                                             >  3:145355/1‑148 (MQ=255)
                                                                           ttATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGa                                                                             <  4:76115/144‑1 (MQ=255)
                                                                           ttATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGa                                                                             >  3:76115/1‑144 (MQ=255)
                                                                             aTGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTc                                                                      >  2:125669/1‑149 (MQ=255)
                                                                                    aTGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAg                                                                                                                                                    <  4:281408/64‑1 (MQ=255)
                                                                                    aTGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAg                                                                                                                                                    >  3:281408/1‑64 (MQ=255)
                                                                                     taaggcgTACACGCCGCATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACcagca                                                               >  3:187565/4‑148 (MQ=39)
                                                                                     tGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACcagcag                                                              <  1:173080/149‑1 (MQ=255)
                                                                                         gcgTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCa                                                           <  4:329428/148‑1 (MQ=255)
                                                                                              tACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGt                                                     <  1:125286/149‑1 (MQ=255)
                                                                                               aCGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATTTCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGt                                                     <  3:34898/148‑1 (MQ=255)
                                                                                                  ccTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTg                                                  >  4:152619/1‑148 (MQ=255)
                                                                                                  ccTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTg                                                  <  3:130899/148‑1 (MQ=255)
                                                                                                   cTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCa                                                                                                     <  1:48480/96‑1 (MQ=255)
                                                                                                   cTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGc                                                 <  4:336683/148‑1 (MQ=255)
                                                                                                   cTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCt                                                <  1:91842/149‑1 (MQ=255)
                                                                                                   cTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCt                                                <  1:114779/149‑1 (MQ=255)
                                                                                                    ttATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATAc                                                                                                                              >  3:21407/1‑70 (MQ=255)
                                                                                                    ttATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATAc                                                                                                                              <  4:21407/70‑1 (MQ=255)
                                                                                                    ttATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCAACAGCAGGCACTTGGTTTGCTg                                               >  1:170725/1‑149 (MQ=255)
                                                                                                      aTCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGt                                             <  1:358243/149‑1 (MQ=255)
                                                                                                       tCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGt                                             <  4:371482/148‑1 (MQ=255)
                                                                                                        ccGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTaa                                           <  2:17729/149‑1 (MQ=255)
                                                                                                          ggCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACtt                                                        <  4:239947/134‑1 (MQ=255)
                                                                                                          ggCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACtt                                                        >  3:239947/1‑134 (MQ=255)
                                                                                                          ggCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAAt                                          <  4:236686/148‑1 (MQ=255)
                                                                                                               aCGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCa                                    <  1:131437/149‑1 (MQ=255)
                                                                                                                  gTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGc                                        <  1:50567/142‑1 (MQ=255)
                                                                                                                  gTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGc                                        >  2:50567/1‑142 (MQ=255)
                                                                                                                    cAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGcatc                               <  1:149650/149‑1 (MQ=255)
                                                                                                                       gTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCatcatc                            >  1:296914/1‑149 (MQ=255)
                                                                                                                       gTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCatcatc                            >  1:242082/1‑149 (MQ=255)
                                                                                                                            cGTATGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATc                                                                                                      <  4:351394/70‑1 (MQ=255)
                                                                                                                            cGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATc                                                                                                      >  3:351394/1‑70 (MQ=255)
                                                                                                                            cGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTtgtg                       <  2:197206/149‑1 (MQ=255)
                                                                                                                            cGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTtgtg                       >  2:135829/1‑149 (MQ=255)
                                                                                                                              tAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGt                                                                                     <  1:145357/85‑1 (MQ=255)
                                                                                                                              tAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGt                                                                                     >  2:145357/1‑85 (MQ=255)
                                                                                                                              tAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTgg                      >  4:71020/1‑148 (MQ=255)
                                                                                                                               aGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGt                    >  1:281106/1‑149 (MQ=255)
                                                                                                                                  cATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATAGCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGttt                  >  4:234576/1‑148 (MQ=255)
                                                                                                                                  cATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGttt                  <  3:77836/148‑1 (MQ=255)
                                                                                                                                       tgcgACGCTTACGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGcatcat                             >  4:247589/4‑132 (MQ=255)
                                                                                                                                       tAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGa             >  4:190285/1‑148 (MQ=255)
                                                                                                                                            cgcgTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGAt        >  3:340008/1‑148 (MQ=255)
                                                                                                                                            cgcgTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATa       <  2:352630/149‑1 (MQ=255)
                                                                                                                                             gcgTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATAc      >  1:354174/1‑149 (MQ=255)
                                                                                                                                                 aaGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGa  >  1:119718/1‑149 (MQ=255)
                                                                                                                                                 aaGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGa  >  1:203374/1‑149 (MQ=255)
                                                                                                                                                  aGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGa  <  3:234576/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGA  >  NC_000913/3754781‑3755074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: