Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,015,333 A→T 14.3% intergenic (+19/+21) metE → / ← ysgA 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase/putative carboxymethylenebutenolidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,015,3330AT14.3% 120.2 / 12.7 42intergenic (+19/+21)metE/ysgA5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase/putative carboxymethylenebutenolidase
Reads supporting (aligned to +/- strand):  ref base A (15/21);  new base T (2/4);  total (17/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

CATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGT  >  NC_000913/4015194‑4015478
                                                                                                                                           |                                                                                                                                                 
cATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTATTACCAccc                                                                                                                                           <  3:111241/148‑1 (MQ=255)
   tCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTATTACCACCCGGt                                                                                                                                        <  3:325691/148‑1 (MQ=255)
   tCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTc                                                                                                                                       <  1:7016/149‑1 (MQ=255)
    ccGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTc                                                                                                                                       <  3:219814/148‑1 (MQ=255)
      ggCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCtt                                                                                                                                     <  4:400945/148‑1 (MQ=255)
         agagCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTtc                                                                                                                                  <  4:352372/148‑1 (MQ=255)
            gcgcCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCAt                                                                                                                              >  1:131970/1‑149 (MQ=255)
                 tgtgGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACa                                                                                                                          <  3:96274/148‑1 (MQ=255)
                       tCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGActt                                                                                                                   >  2:343482/1‑149 (MQ=255)
                       tCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGAct                                                                                                                    <  3:391770/148‑1 (MQ=255)
                         aaCCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGActtc                                                                                                                  <  3:323371/148‑1 (MQ=255)
                          aCCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGActtct                                                                                                                 <  4:69831/148‑1 (MQ=255)
                            ccGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACttcttc                                                                                                               >  4:34286/1‑148 (MQ=255)
                                       ccTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTATTACCACCCGGt                                                                                                                                        <  3:113075/112‑1 (MQ=255)
                                       ccTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTATTACCACCCGGt                                                                                                                                        >  4:113075/1‑112 (MQ=255)
                                       ccTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTg                                                                                                    <  4:384417/148‑1 (MQ=255)
                                        cTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGc                                                                                                   <  3:323285/148‑1 (MQ=255)
                                        cTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCt                                                                                                  >  2:309717/1‑149 (MQ=255)
                                        cTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTAGTATTACCACCCGGtt                                                                                                                                       <  2:245307/112‑2 (MQ=255)
                                        cTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTAGTATTACCACCCGGtt                                                                                                                                       >  1:245307/1‑111 (MQ=255)
                                                  gcgGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTc                                                                                        <  2:53149/149‑1 (MQ=255)
                                                          ccAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATAc                                                                                >  1:29695/1‑149 (MQ=255)
                                                                     cgcgGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTgccagcc                                                                      >  3:305508/1‑148 (MQ=255)
                                                                         gCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCAtctt                                                                 <  2:405205/149‑1 (MQ=255)
                                                                         gCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGAGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCAGACGCTGCCAGCCAtct                                                                  >  3:345251/1‑148 (MQ=255)
                                                                          cACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCAtcttc                                                                <  1:343482/149‑1 (MQ=255)
                                                                             tGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTgccagcc                                                                      <  1:4162/140‑1 (MQ=255)
                                                                             tGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTgccagcc                                                                      >  2:4162/1‑140 (MQ=255)
                                                                                 gAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGAt                                                         >  1:181905/1‑149 (MQ=255)
                                                                                  aaCATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATg                                                        <  1:309717/149‑1 (MQ=255)
                                                                                   aCATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATg                                                        <  3:34286/148‑1 (MQ=255)
                                                                                          gCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAAGACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGa                                                <  2:336028/149‑1 (MQ=255)
                                                                                           cAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGa                                                <  3:273830/148‑1 (MQ=255)
                                                                                               cggcgCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACg                                                                               >  4:77640/1‑113 (MQ=255)
                                                                                               cggcgCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACg                                                                               <  3:77640/113‑1 (MQ=255)
                                                                                                                 gggggTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGt                          <  3:346406/148‑1 (MQ=255)
                                                                                                                      tAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTgcca                                                                         >  1:377043/1‑96 (MQ=255)
                                                                                                                      tAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTgcca                                                                         <  2:377043/96‑1 (MQ=255)
                                                                                                                         aaTCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAatgca                  >  3:310111/1‑148 (MQ=255)
                                                                                                                           tCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAatgcatgc               >  1:393217/1‑149 (MQ=255)
                                                                                                                             cAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGccc             >  2:151444/1‑149 (MQ=255)
                                                                                                                                        gtAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGt  >  1:117536/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                                 
CATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGT  >  NC_000913/4015194‑4015478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑