Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,070,410	C→A	12.2%	intergenic (‑9/+105)	yihR ← / ← yihS	putative sulphoquinovose mutarotase/sulphoquinovose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,070,410	0	C	A	12.2%	129.1 / 8.7	41	intergenic (‑9/+105)	yihR/yihS	putative sulphoquinovose mutarotase/sulphoquinovose isomerase
Reads supporting (aligned to +/- strand):  ref base C (18/18);  new base A (2/3);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

AACCCTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTG  >  NC_000913/4070263‑4070555                                                                                                                                                    |                                                                                                                                                  aaCCCTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTccc                                                                                                                                                  <  1:182082/149‑1 (MQ=255) aaCCCTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTccc                                                                                                                                                  >  1:64053/1‑149 (MQ=255)  aCCCTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTccc                                                                                                                                                  >  3:310959/1‑148 (MQ=255)     cTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGa                                                                                                                                              >  1:381267/1‑149 (MQ=255)      tGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGa                                                                                                                                              >  4:116894/1‑148 (MQ=255)        aaaGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCACGGGAGGc                                                                                                                                           <  2:251198/149‑1 (MQ=255)            gTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGa                                                                                                                                        <  3:144617/148‑1 (MQ=255)             ttAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCACGGGAGGCTgta                                                                                                                                       <  3:259321/148‑3 (MQ=255)              tAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACAT‑CAGCCTCACGGGAGGCTgtatg                                                                                                                                     <  3:403868/148‑5 (MQ=255)                  tCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCaaa                                                                                                                                 <  1:121530/149‑1 (MQ=255)                        aGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCaaa                                                                                                                           >  2:226280/1‑149 (MQ=255)                             aGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACt                                                                                                                       >  3:333076/1‑148 (MQ=255)                                aCCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCa                                                                                                                    >  4:321808/1‑148 (MQ=255)                                        tCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGt                                                                                                           >  1:379769/1‑149 (MQ=255)                                           caATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTccc                                                                                                        <  2:289531/149‑1 (MQ=255)                                           caATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTcc                                                                                                         <  3:74103/148‑1 (MQ=255)                                            aaTGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTccc                                                                                                        <  3:295129/148‑1 (MQ=255)                                             aTGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTcccc                                                                                                       >  3:172453/1‑148 (MQ=255)                                              tGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCt                                                                                                      <  4:171999/148‑1 (MQ=255)                                                 tCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGc                                                                                                   >  3:285295/1‑148 (MQ=255)                                                    cgTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCt                                                                                               <  1:237803/149‑1 (MQ=255)                                                      tGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCtt                                                                                              <  3:116894/148‑1 (MQ=255)                                                         tAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTggg                                                                                          <  2:379769/149‑1 (MQ=255)                                                         tAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTgg                                                                                           <  4:310959/148‑1 (MQ=255)                                                          aaTCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTggg                                                                                          >  4:292105/1‑148 (MQ=255)                                                           aTCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGga                                                                                        <  2:256582/149‑1 (MQ=255)                                                              ccccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGgaga                                                                                      <  4:333076/148‑1 (MQ=255)                                                                    ggCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGACAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTa                                                                                <  3:156443/148‑1 (MQ=255)                                                                                  tCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGaaaa                                                                  >  4:386758/1‑148 (MQ=255)                                                                                       ccACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGc                                                             >  4:189554/1‑148 (MQ=255)                                                                                                tGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGagag                                                                                     >  2:399056/1‑115 (MQ=255)                                                                                                tGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGagag                                                                                     <  1:399056/115‑1 (MQ=255)                                                                                                  cATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTc                                                  <  3:386758/148‑1 (MQ=255)                                                                                                    tatTGGTGATTTGAATACAAGTCCCCTTATTTAACGACTAACGCCCTCCCGTGAGGCTAATGTAAAACCCAAAAACTGCACCGGCCGGTCCCCTCGCCCTTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGa                                               <  1:323700/149‑1 (MQ=255)                                                                                                    tatTGGTGATTTGAATAAAAGGCACTTTAATTACCGACATACGGCCCCCCGTGGGGCTGATGTCAAACCCAAAAACGGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTGGGGAGAGGGAAAAACCCGCACCCTATTTTCAGa                                               <  2:389244/149‑1 (MQ=38)                                                                                                            aTTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCGGATCAGTTa                                        >  3:253163/1‑148 (MQ=255)                                                                                                            aTTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGCGGAAAACCCGCACCCGATTTTCAGATCAGatat                                       >  2:200684/1‑149 (MQ=255)                                                                                                                  aaaCAAGCCCCCTTATTTAACCCCATACACCCCCCCGGGAGGCTGATGTAAAACCCAAAACCTGCACCGGACGGCCCCCTCGCCCCTTGGGGGAGGGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGc                                  <  3:321808/146‑1 (MQ=255)                                                                                                                   tACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCa                                 >  3:229137/1‑148 (MQ=255)                                                                                                                                           tACAGCCTCCCGTGAGGCTGTTGGCAAAACCAAATACTGCACCGGACGGTCCCCTCGCCCCTTTGGGAAGTGGTTTAGCGAGAGCGGATAACCCGCACCCTATTTTCAGATCAGTTATCTCGTATTAATATCCAGCAGACCCGCTGCa         >  4:251016/1‑148 (MQ=255)                                                                                                                                           tACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCaa        >  1:234612/1‑149 (MQ=255)                                                                                                                                           atcAGCCTCACGGGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCaa        >  1:248005/3‑149 (MQ=255)                                                                                                                                            tcAGCCTCACGGGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCaa        >  3:206944/2‑148 (MQ=255)                                                                                                                                                  tCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTg  >  3:378268/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                  AACCCTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTG  >  NC_000913/4070263‑4070555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑