Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 374,683 G→C 9.7% P272P (CCG→CCC) ‡ mhpE → 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913374,6830GC 9.7% 130.9 / 5.0 41P272P (CCG→CCC) ‡mhpE4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I
Reads supporting (aligned to +/- strand):  ref base G (19/18);  new base C (2/2);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

GAATGGGCGCGGGCGCAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCG  >  NC_000913/374544‑374828
                                                                                                                                           |                                                                                                                                                 
gAATGGGCGCGGGCGCAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTc                                                                                                                                          <  2:251836/149‑1 (MQ=255)
     ggcgcgggcgcAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACTCGTACCGGTCGATcc                                                                                                                                     <  2:298361/149‑2 (MQ=255)
               cAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAAcgct                                                                                                                            >  3:199534/1‑148 (MQ=255)
                aGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACTGGTACGAGTCGATCGCGAAAcgctgg                                                                                                                          >  2:332305/1‑149 (MQ=255)
                      cGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAAcgctggcgctgg                                                                                                                    <  1:401195/149‑1 (MQ=255)
                      cGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAAcgctggcgctg                                                                                                                     <  3:163082/148‑1 (MQ=255)
                       gCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGCCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTggg                                                                                                                   <  2:121351/149‑1 (MQ=255)
                        cACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTggg                                                                                                                   <  4:400917/148‑1 (MQ=255)
                        cACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTggg                                                                                                                   >  4:334254/1‑148 (MQ=255)
                         aCCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGAt                                                                                                                 >  2:9788/1‑149 (MQ=255)
                              tGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGc                                                                                                             <  3:245886/148‑1 (MQ=255)
                              tGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCt                                                                                                            >  1:187505/1‑149 (MQ=255)
                                     gtTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACTCGTACCGGTCGATcctg                                                                                                                                   >  1:106292/1‑116 (MQ=255)
                                     gtTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACTCGTACCGGTCGATcctg                                                                                                                                   <  2:106292/119‑4 (MQ=255)
                                     gtTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGtt                                                                                                      >  3:126313/1‑148 (MQ=255)
                                      tttATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGttt                                                                                                     >  3:373398/1‑148 (MQ=255)
                                           tGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGa                                                                                               <  1:392052/149‑1 (MQ=255)
                                                            tGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACtg                                                                               <  3:334254/148‑1 (MQ=255)
                                                             gggCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACtgt                                                                              <  4:351859/148‑1 (MQ=255)
                                                                cTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGaaa                                                                          <  2:43828/149‑1 (MQ=255)
                                                                 tGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAAc                                                                         <  1:50293/149‑1 (MQ=255)
                                                                            gACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGgcgc                                                              >  2:392242/1‑149 (MQ=255)
                                                                             aCCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGgcgc                                                              >  3:289170/1‑148 (MQ=255)
                                                                               cGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGt                                                            <  4:126313/148‑1 (MQ=255)
                                                                                  tctcTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTAt                                                         >  4:27578/1‑148 (MQ=255)
                                                                                           gTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGaaa                                                                          >  1:185624/1‑122 (MQ=255)
                                                                                           gTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGaaa                                                                          <  2:185624/122‑1 (MQ=255)
                                                                                                  gATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGa                                        <  1:84469/149‑1 (MQ=255)
                                                                                                    tGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGat                                       >  4:102623/1‑148 (MQ=255)
                                                                                                            acgacCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGtt                              <  2:104535/149‑1 (MQ=255)
                                                                                                              gacCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTg                             <  4:373398/148‑1 (MQ=255)
                                                                                                                ccTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGc                          >  2:380517/1‑149 (MQ=255)
                                                                                                                 cTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCt                         >  1:369883/1‑149 (MQ=255)
                                                                                                                  tGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCt                         >  4:360370/1‑148 (MQ=255)
                                                                                                                      gCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCa                    >  1:189435/1‑149 (MQ=255)
                                                                                                                             ttGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTg                        >  3:95955/1‑138 (MQ=255)
                                                                                                                             ttGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTg                        <  4:95955/138‑1 (MQ=255)
                                                                                                                                   gATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGt       >  2:48191/1‑149 (MQ=255)
                                                                                                                                    aTCGACTCGTACCGGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGt       >  4:400807/1‑148 (MQ=255)
                                                                                                                                    aTCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGt       <  4:199534/148‑1 (MQ=255)
                                                                                                                                         ccGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTggcg  >  3:403866/1‑148 (MQ=255)
                                                                                                                                           |                                                                                                                                                 
GAATGGGCGCGGGCGCAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCG  >  NC_000913/374544‑374828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑