Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 847,203 T→C 9.8% intergenic (‑84/+55) glnP ← / ← glnH glutamine transporter subunit/glutamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913847,2030TC 9.8% 105.9 / 3.9 41intergenic (‑84/+55)glnP/glnHglutamine transporter subunit/glutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (20/17);  new base C (2/2);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

TTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACG  >  NC_000913/847059‑847350
                                                                                                                                                |                                                                                                                                                    
ttttGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAACCctc                                                                                                                                                  >  1:126121/1‑149 (MQ=255)
      cACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTcccc                                                                                                                                             >  3:123310/1‑148 (MQ=255)
          ttCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑gg                                                                                                                                       >  1:79032/1‑149 (MQ=255)
          ttCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAACCCTCTcccctatgg                                                                                                                                        >  1:305041/1‑144 (MQ=255)
           tCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCCTA‑gg                                                                                                                                       >  3:377007/1‑148 (MQ=255)
               tCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGgag                                                                                                                                   >  4:172465/1‑148 (MQ=255)
                  gAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAACCCTCTCCCCTAT‑GGGGAGAgga                                                                                                                               <  2:126121/149‑2 (MQ=255)
                      cGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAACCCTCTCCCC‑TATGGGGAGAggattag                                                                                                                           <  2:305041/149‑6 (MQ=255)
                          aTGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGcc                                                                                                                        <  3:371038/148‑1 (MQ=255)
                                 gCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGgaga                                                                                                                                  >  1:202268/1‑131 (MQ=255)
                                 gCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGgaga                                                                                                                                  <  2:202268/131‑1 (MQ=255)
                                    aGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGtttt                                                                                                              <  3:50902/148‑1 (MQ=255)
                                              ccAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCATCCTAATCCTCTCCCCATA‑GGGGAGAgg                                                                                                                                <  3:347475/120‑1 (MQ=255)
                                              ccAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCct                                                                                                                                                   <  3:359808/102‑1 (MQ=255)
                                              ccAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCct                                                                                                                                                   >  4:359808/1‑102 (MQ=255)
                                              ccAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAgg                                                                                                                                >  4:347475/1‑120 (MQ=255)
                                                    aaaCTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGttat                                                                                              <  3:172465/148‑1 (MQ=255)
                                                          cATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCg                                                                                       <  2:43117/149‑1 (MQ=255)
                                                            tatGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCgg                                                                                      <  3:390382/148‑1 (MQ=255)
                                                                ttgttCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCa                                                                                  >  1:10574/1‑148 (MQ=255)
                                                                ttgttCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCa                                                                                  <  2:10574/148‑1 (MQ=255)
                                                                   ttCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGgaga                                                                                                                                  >  2:117160/1‑97 (MQ=255)
                                                                   ttCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGgaga                                                                                                                                  <  1:117160/97‑1 (MQ=255)
                                                                   ttCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGttt                                                                                                               >  2:334477/1‑116 (MQ=255)
                                                                   ttCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGttt                                                                                                               <  1:334477/116‑1 (MQ=255)
                                                                   ttCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTAc                                                                              >  1:255634/1‑149 (MQ=255)
                                                                       tGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGga                                                                                                                                    <  2:377305/91‑1 (MQ=255)
                                                                       tGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGga                                                                                                                                    >  1:377305/1‑91 (MQ=255)
                                                                          tACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAAcc                                                                        >  4:169380/1‑148 (MQ=255)
                                                                              gtggtgTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGtt                                                                                    >  2:331767/1‑132 (MQ=255)
                                                                              gtggtgTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGtt                                                                                    <  1:331767/132‑1 (MQ=255)
                                                                                   gtAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTa                                                              <  1:231508/149‑1 (MQ=255)
                                                                                      aataatCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGAt                                                           >  1:388020/1‑149 (MQ=255)
                                                                                         aatCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTc                                                        >  2:58861/1‑149 (MQ=255)
                                                                                            cAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGtt                                                     >  1:35907/1‑149 (MQ=255)
                                                                                                              tGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTcgc                                    >  4:138170/1‑148 (MQ=255)
                                                                                                                          gggTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCa                        <  3:164085/148‑1 (MQ=255)
                                                                                                                                       tCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTAGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTCTTCAACGCGCCGTTGAc           >  4:45997/1‑148 (MQ=255)
                                                                                                                                           ccTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTcc                                            <  2:242301/111‑1 (MQ=255)
                                                                                                                                           ccTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTcc                                            >  1:242301/1‑111 (MQ=255)
                                                                                                                                               aTCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACg  <  1:361665/149‑1 (MQ=255)
                                                                                                                                                tCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACg  <  4:340707/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATA‑GGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACG  >  NC_000913/847059‑847350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑