Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,129,994 Δ1 bp coding (160/2433 nt) metL → Bifunctional aspartokinase/homoserine dehydrogenase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,129,9940A.96.3% 323.1 / 6.9 85coding (160/2433 nt)metLBifunctional aspartokinase/homoserine dehydrogenase 2
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base . (41/38);  total (45/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGCCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAA  >  NC_000913/4129735‑4130259
                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                          
tctcCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTaa                                                                                                                                                                                                                                                            <  2:261009/275‑1 (MQ=255)
      agaCGCTTGTGCGTAGCTCCACCGGTATGGAGGATGGCGAAGTGTTAAGTGCAGACCTGGAAAATGGCTTCCGGGCTGCAAAAAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGtt                                                                                                                                                                                                                                                      <  3:310683/275‑1 (MQ=255)
          gctggtGCGTAACTCAACAGGTAATGAAGATGGGGAAGATTTAATTGCCGACCTGGCAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGaaa                                                                                                                                                                                                                                                  <  3:60262/275‑1 (MQ=255)
          gctgctgctGATGTCGATCGGTATTTAAGATGGCAAAGATTTACTTGCAGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTATCACCCTAACCAGTTGATTAACTGGTTGaaa                                                                                                                                                                                                                                                  <  2:531569/275‑1 (MQ=255)
            tgctgcGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTa                                                                                                                                                                                                                                               >  2:80614/1‑276 (MQ=255)
              gtgcGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAg                                                                                                                                                                                                                                             <  2:325754/275‑1 (MQ=255)
                   tATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGGGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGAGGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGCTTACCTTGTTGAAAATAAGCCa                                                                                                                                                                                                                                          >  1:63484/1‑274 (MQ=255)
                    tactacACCTGTAGTGAGGAGTGCGAATATTTAATTGCTGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGa                                                                                                                                                                                                                                        <  2:169820/270‑1 (MQ=255)
                          aCCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCg                                                                                                                                                                                                                                 <  2:63252/276‑1 (MQ=255)
                           ccGGTCTTGAAGATGGCGAAGATTTAATTGCCGACCTGGCAAATGGCTTCCGGGCTGCAAACCAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGAAGGAGTCTGGCGGAGGTGAAGTGTTATTTGCGTGTCGCGGGCAGTATGGCGGAGTACTCGCCGCCCGACGATTTGATGGTGGTTTCCTCCGCCGGTAGCACCCTAACCAGCTGATTAACTGGTTGAAATTAAGCccgaccgaac                                                                                                                                                                                                                                  >  3:18482/1‑272 (MQ=255)
                           ccGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTCCTCTCAGCCTGAGGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTGGAAACTAAGCGAGACCGGTCg                                                                                                                                                                                                                                 >  4:246871/1‑275 (MQ=255)
                           ccGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATCTGAGGGTGGTTTCTGCCGCCGGTTGCACCCCAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACAGATCg                                                                                                                                                                                                                                 >  1:390369/1‑275 (MQ=255)
                           ccGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGGGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTGCCGCCGCCGGTAGCACCCTAACCAGTGGTAGAACTGGTTGAAACTAAGCCAGACCGATCGt                                                                                                                                                                                                                                >  4:476761/1‑276 (MQ=255)
                                 ttGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAACGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGAGGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAGACTAATCCAGACCAATCTtctctc                                                                                                                                                                                                                          >  3:35144/1‑275 (MQ=255)
                                         ggCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGTTTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGATTTCCGCCGCCGGTAGCACTCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATc                                                                                                                                                                                                                  >  2:123650/1‑275 (MQ=255)
                                         ggCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTCCTATCAGCCTGACGATATGATGGTGGTTTCCGCCGCGGGTAGCCCCCTAACCAGTTGATTAGCTGGTTGAAACTAAGCAGGAGCGATCTTCTCTGGGCGCATc                                                                                                                                                                                                                  >  4:349108/1‑275 (MQ=255)
                                         ggCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACAGATCGTCTCTCTGCGCATCa                                                                                                                                                                                                                 >  2:492529/1‑276 (MQ=255)
                                         ggCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTACCACCCTAACCAGTTGACTCACTGGTTCGAACTAAGCCAGACCGATCCtctcctttcccatc                                                                                                                                                                                                                  >  4:274660/1‑265 (MQ=255)
                                         ggCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGCTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATc                                                                                                                                                                                                                  >  2:124060/1‑275 (MQ=255)
                                          gCGTAGCTTTAATTGCCGACCGGGAAAAGGGCTTCCGGGGTGGAAACAAGGGGGAAAAATGAGTGTGATTGCGGAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCGGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGACGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCa                                                                                                                                                                                                                 <  3:299995/275‑1 (MQ=255)
                                           cGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCTTCa                                                                                                                                                                                                                 >  1:85245/1‑274 (MQ=255)
                                             gggATGTAATTGCGGAACGGGAAAATGGGTGCCGGGCTGCGAACAAGGGGGGAAGATGGGTTGTGTTGGGGAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCGGTAGTCTGGCTGATGTGAAGTGTTAGTTGCGGGTCACGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTCGAAACTAAGCCAGACCGATCGTCTCTCTGCGTATCAGGt                                                                                                                                                                                                              <  4:468934/273‑1 (MQ=255)
                                                    aaTTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGGAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCCGAGGTGACGTGTTATTTGCGTGTCGCGGGCATTATGGCCGAGTACTCGCAGCCTGACGATATGATGGTTGTTTCCGCCGCCGGTCGCACACTAACCAGTCGATTAACTGGTTGCATCGAAGACATACAGCTTGCCTCTCAGCGCAtatgtgtaagcaa                                                                                                                                                                                                       >  3:241893/1‑263 (MQ=255)
                                                     aTTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTCACTGGTTGAAACTAAGCCAGACCGATCGTCTCCCTGCGCCTCGGGTTcaacaac                                                                                                                                                                                                      >  4:61473/1‑274 (MQ=255)
                                                              cTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGGGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTTGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGt                                                                                                                                                                                              <  2:246871/274‑1 (MQ=255)
                                                                           tacGGGCTGCAAAAAAGGGGTAAAAAGGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGAt                                                                                                                                                                               <  2:274660/274‑1 (MQ=255)
                                                                           tCCTGGCTGCAAAGAAGGGGGAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCTTCAGCTGCATAAATTTGGTGGCATTAGTCTGGTTGATGTGAAGTGTTATTGGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCNAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGAt                                                                                                                                                                               <  4:335683/276‑1 (MQ=255)
                                                                           tCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCGGTGc                                                                                                                                                                                  >  1:120867/1‑273 (MQ=255)
                                                                           tCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCGGTGCg                                                                                                                                                                                 >  2:265999/1‑274 (MQ=255)
                                                                           tCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCCCTGCGCATCAGTTTCAACAAACGCTCCGGCGCTATCAGtccta                                                                                                                                                                                >  3:275741/1‑271 (MQ=255)
                                                                           tCCGGGCTGCAAACAAGGGGTAAAAATGAGGGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGAt                                                                                                                                                                               <  2:160988/276‑1 (MQ=255)
                                                                                   gCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCTTGACGATATGATGGTGGTTTCCGCCGCCGGTAGAACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGGGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGCTCGATCCGAtt                                                                                                                                                                         >  1:116/1‑274 (MQ=255)
                                                                                        agaGGGGTAAAAAAGGGGGAGGTTGGGCAGGAAGGGGGGAAAGGTGGGCAGGTGCATAAGGTTGGTGTAAGGAGTCTTGATGATGGTAAGTGTTATTGGCGGTGTGCGGGCAGTATGGCGGAGTACTCTCATCCTGAGGATATGAGGGTGGTTGCCGCCGCCGGTAGCACCCTCACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGt                                                                                                                                                                   <  3:116/273‑1 (MQ=255)
                                                                                              gTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTTCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCt                                                                                                                                                              >  3:313736/1‑274 (MQ=255)
                                                                                              gTAAAAATGAGTGTGATTGCGCAGGCAGGGGAGAAAGGTCGTAAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCt                                                                                                                                                              <  1:286012/274‑1 (MQ=255)
                                                                                                       aGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTCCCCGCTgca                                                                                                                                                    >  2:174282/1‑273 (MQ=255)
                                                                                                       aGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTAgcccatga                                                                                                                                                     >  1:90113/1‑269 (MQ=255)
                                                                                                       aGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACACTAGCCAGTTGATGAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTga                                                                                                                                                     >  1:106727/1‑274 (MQ=255)
                                                                                                          tgtgATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCGGCCGCCGGTAGCACCCTCACCAGGTGAGTAACTGGTTGAAACTAAGCCAAACCGATCGTCTCGATGCGCATCAGGTTAAACAAGCGCTGCGTGGCTCACAGTGCGATCAGATTAGCGGTCTGCCACCTGCGgaaga                                                                                                                                                  >  4:163666/1‑275 (MQ=255)
                                                                                                          tgtgATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTGACTGGTTGACACTAAGCCAGACAGATCGTCTCTCTGCGCATCGTGTTCAAATACCGCTGCGTCCCGCACAGTGCGATCTGATTAGCGGTATGCTACCCGCTgctgac                                                                                                                                                 >  4:113409/1‑271 (MQ=255)
                                                                                                          tgtgATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGCTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTAGCTATCAGTCCGATCTGATTAGCGGTCTGCTACCCGCTgaaga                                                                                                                                                  >  1:114905/1‑275 (MQ=255)
                                                                                                              aTTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGcc                                                                                                                                              >  2:285659/1‑275 (MQ=255)
                                                                                                                 gcgagggcaggGGCGAAATGTCGTCAGTTCGATAAATTTGGTGGAAGGAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGAt                                                                                                                                           <  3:120867/270‑1 (MQ=255)
                                                                                                                  cgcAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTACCCAGTTGATCAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTCCAACAAACGTCGCGTCGCTATCAGTGCGACCAGAATAGCGGTCTGCTACCCGCTGAAGAACCCGAtg                                                                                                                                          >  3:241091/1‑274 (MQ=255)
                                                                                                                   gcatgcagGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGGCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGTGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCATCCTATCCAGTTGATTAACTGGTTGAAACTAAGCCAGCCCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGc                                                                                                                                        <  2:113409/276‑1 (MQ=255)
                                                                                                                         cggggCGGAAAGGTCGTCAGCTGAGTAAATTTGGTGGTAGTAGTCTGGCTGATGTGAAGGGTTATTTGCTGGTCGCGGGACTGATGGCGGAGTACTCTCAGCCTGACGTTATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCAt                                                                                                                                   <  4:346169/275‑1 (MQ=255)
                                                                                                                         aggGGCGAAAAGTCGTCATCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCAt                                                                                                                                   <  1:235646/275‑1 (MQ=255)
                                                                                                                            ggCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAgc                                                                                                                               >  2:369189/1‑276 (MQ=255)
                                                                                                                                          cAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGc                                                                                                                   <  2:476761/275‑1 (MQ=255)
                                                                                                                                                cATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGACCTGATTAGCGGTCTGCTACCCGCTga                                                                                                                                                     >  2:183335/1‑235 (MQ=255)
                                                                                                                                                                    tCTGGCTGTTGTGTAGGTGTTGTTGGGTGGCGCGGGCATTCTGGCGGGTTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTc                                                                                        <  4:369189/276‑1 (MQ=255)
                                                                                                                                                                       ggCTGATGGGGAGTGTTAGTGGCGTGTCGCGGGCATCAGGGCGGAGTACTCTCAACCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCCCCCTAACCAGTTGATTAACTGGTTAAAACTAAGCCGGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGa                                                                                      <  3:114905/275‑1 (MQ=255)
                                                                                                                                                                         ggtaTGTGAAGGGTTATTGGCGGTTGGCGGGCATTATGGCGGAGTACTCTCAGCTTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCGCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACa                                                                                    <  4:80614/272‑1 (MQ=255)
                                                                                                                                                                             tgtgAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGAcc                                                                                                                                                                                                                                      >  2:441847/1‑126 (MQ=255)
                                                                                                                                                                              gtgAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGGGCGCCTGGCGGCGCTGCGCGACAGCGGTa                                                                              >  2:219007/1‑276 (MQ=255)
                                                                                                                                                                                gAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCGGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGAt                                                                                                                                                                                                                                   <  4:441847/126‑1 (MQ=255)
                                                                                                                                                                                       ttATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGCAGCCGATAGCCTCATTAGGGCTTTTGTAAGCGACCTTGAGCGCCTGGCGGATCTGCTCGTCCGCGGTTTTCacga                                                                       >  1:321981/1‑275 (MQ=255)
                                                                                                                                                                                         aTTTGCGGGGCGCGGGCATTAGTGCGGAGTACTTCCAGCCTGACGCTCTGATGGTGGTTTCCGCCGGCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGAGCGTCTCTCTGCGCATCAGGTTCAACAAACGCGGCGTCGCTATCAGTGCGATCTGAGTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAacgacg                                                                     <  3:85245/275‑1 (MQ=255)
                                                                                                                                                                                          tttGCGTGTCGCGGGCATTATGGCGGATTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGc                                                                    <  1:264233/275‑1 (MQ=255)
                                                                                                                                                                                           tggCGTGGCGCGTGCATTATGGCGGAGTACGCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCa                                                                   <  2:468900/273‑1 (MQ=255)
                                                                                                                                                                                            ttgcgttgtgcgGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTGGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCa                                                                   <  2:163666/266‑1 (MQ=255)
                                                                                                                                                                                            tGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGCGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAg                                                                  >  3:241177/1‑275 (MQ=255)
                                                                                                                                                                                            tGCGGGTCTCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTTATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAgt                                                                 <  2:349108/276‑1 (MQ=255)
                                                                                                                                                                                                gtgtCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCCCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTCTTCACGACGCTgtgt                                                               >  3:351434/1‑275 (MQ=255)
                                                                                                                                                                                                    cgcgGGAATTATGGCGGAGTACTCTCAGTCTGACGTTATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCCATCGTCTCTCTGCGCATCAGGTTCAACAAAAGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATg                                                            <  3:321981/274‑1 (MQ=255)
                                                                                                                                                                                                       gggCATTAGGGCGGAATACTCTCAGCCTGACGTTATGATGGTTGTTTCCGACGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCgg                                                         <  1:35144/274‑1 (MQ=255)
                                                                                                                                                                                                                  cGGAGTACTCACAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGCCa                                            <  2:61473/276‑1 (MQ=255)
                                                                                                                                                                                                                      gTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGCCACg                                          <  1:313736/274‑1 (MQ=255)
                                                                                                                                                                                                                                   gACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATGAGCGCTTTTGTCTGCGACCTTGAGCGCCTGGCGGCGCTCGTGGACAGCGGTATTAACGACGCATGTTACGCGGGAATGGTGGGGCACgggggcgctaggtcgg                           >  4:277805/1‑264 (MQ=255)
                                                                                                                                                                                                                                    aCGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAGGAAGCCGATAGCCTCTTTAGCGCTTTTGTCAGCGCCCTTGCGCGCCTGTCGGCGCTGCTCGACAGCGGTATTAACGCCCCAGTGTATGCGGAAGTGGTGGGCCAGGGGGACGTATGGTc                             >  2:300723/1‑273 (MQ=255)
                                                                                                                                                                                                                                    aCGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGa                                                                                      >  4:44288/1‑216 (MQ=255)
                                                                                                                                                                                                                                    aCGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGCCAGCGGCATTAACGACGCAGTGATTGCGGAAGTGGGGGGCCACGGGGGAGTGTggcc                             >  4:548362/1‑271 (MQ=255)
                                                                                                                                                                                                                                    aCGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGCCACGGGGAAGTATGGTCgg                           <  1:238131/275‑1 (MQ=255)
                                                                                                                                                                                                                                    aCGATATGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGCCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGCAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGCCGCAGTGTCTGCGGAAGTGGTGGGCCACGGGGAGGTATGGt                              >  3:378649/1‑272 (MQ=255)
                                                                                                                                                                                                                                      gtcaggccggtggtCTCCGCCGCACGTACAACCATAACCAGGCTAATTACAGATTGAGAGTAAGGCAGACCGTAGTGAGCTCGGCGCCTCAGGTCCAACAAACGCTGCGTCGTTATGAGGGCGATCGGATTAGCGTTCTGCTACCCGCGGTAGAATGCGATGGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGGGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGGGGTTGGCCACGGGGAAGTATGGTCgg                           <  3:188768/265‑1 (MQ=255)
                                                                                                                                                                                                                                       atatGATGGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAg                                                                                   <  2:44288/216‑1 (MQ=255)
                                                                                                                                                                                                                                          tgatgGTGGTTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAATTGGTGGGCCACGGGGAAGTATGGTCGGCCCGt                      >  1:388209/1‑274 (MQ=255)
                                                                                                                                                                                                                                             tggtggTTTCAGCCGCCGGTAACACCCTATCCATTGGATTAACTGGTTGAAACTCAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGATATCAGTGCGATCTGCTTAGCGGTCTGCTACCCGCGGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGCCACGGGGAAGTATGGTCGGCACGTCTGAt                 <  4:265999/276‑1 (MQ=255)
                                                                                                                                                                                                                                                  gtTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTgc                                                                                                                                                                                                                       >  3:29889/1‑73 (MQ=255)
                                                                                                                                                                                                                                                  gtTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTTAGCGCCTGGCGGCGCTGCCCGCCAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGCCGCGGGGGAGGCTGGTCGGCACGTCTGCTGTCt             >  3:253024/1‑275 (MQ=255)
                                                                                                                                                                                                                                                  gtTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGcc                                                                                                      >  2:546538/1‑186 (MQ=255)
                                                                                                                                                                                                                                                  gtTTCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGACTAGCCGTCTGCTACCCGCTGAACACGCCGCTAGCCCCATTAGCCCTGTTGCCAGCGCCCTTGCGCGCCCTGCGGCGCTGCCCATACCCGGTAGTAACGCCGCAGTGTAGCCGGAAGTTGTGGGCCACGGGGAGCGAGGATCGGCACgcatcatgcccg            >  4:591611/1‑265 (MQ=255)
                                                                                                                                                                                                                                                     tCCGCCGCCGGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTgg                                                                                                   <  4:546538/186‑1 (MQ=255)
                                                                                                                                                                                                                                                      ccgccgccgGTAGCACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCAt                                                                                                                                                                                                                   <  1:29889/73‑1 (MQ=255)
                                                                                                                                                                                                                                                              ggTAGAACCCTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGCCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTaa  <  1:275741/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                          
TCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGCCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAA  >  NC_000913/4129735‑4130259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: