Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,3801.C94.9% 236.8 / 3.2 81intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (1/3);  new base C (43/32);  total (45/36)
Fisher's exact test for biased strand distribution p-value = 3.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*NC_0009134,296,3802.G93.7% 242.1 / 6.2 81intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (1/4);  new base G (43/31);  total (45/36)
Fisher's exact test for biased strand distribution p-value = 1.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCG  >  NC_000913/4296189‑4296646
                                                                                                                                                                                                  ||                                                                                                                                                                                                                                                                              
tgccgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATTCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTCACCGTTTGTTAATTTTTCaacttc                                                                                                                                                                                          >  2:470930/4‑272 (MQ=255)
          tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                                                                  <  1:231770/274‑1 (MQ=255)
          gatctgccgtatgCCAGACAGCAGAATTGACTAATTTGAGGTACTTTTGTAGATCGAATAGGGCGTTGATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                                                                  <  3:319269/262‑1 (MQ=14)
            gtaGGCCAACGCCAGAGAGCGAAATAGCCTGATTTAGCGTGATTTGGTAGGTCGGATAAGTCATTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGACTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAccc                                                                                                                                                                                 <  1:66631/271‑1 (MQ=14)
                   tACGCCAGACAGCGCAATAGCCTGTTTTAGCCTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCTACATCACCGCCTGATGCGACGCTTAACGTGTCTTATCAGGCCTACGCCAGACATCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                                                                        <  2:217261/275‑1 (MQ=14)
                    aCGCCAGACAGAAGAATAGCCTGATTTTGCGTGAGTTTGTGGGTCGGGTAAAGCGTTTAAGCCGCATCCTACATCAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCTTACGCCAGACAGCGGAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTGTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                                                                        <  3:219754/274‑1 (MQ=14)
                          gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGACTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACAAAAATGCCTGATGAGACCCCGTCCGCGTCGTATCAGGTCTATCTTACCCTTTGTTTAATTTTTATACCTCTTCACATCCGGTCACACACCCtta                                                                                                                                                                  >  3:361833/1‑273 (MQ=11)
                            aggcgcAATAGCCGGATTTATCGTGATTTTGTAGGTCGAATATGGCATTTATGCCGCATCCCCTATCAGCGCCTGATGCGACTCTTACCGCGTCTTATCAGGCCTACGGCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTG‑‑ACGC‑C‑GC‑‑ATCGGAAATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCa                                                                                                                                                                <  3:252044/272‑1 (MQ=17)
                               cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTTATTTAGCGTGATTTTGTAGGTCGGATAAGGCGCTT‑‑A‑‑CGCAGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTACGTTAATTTTTCAACCTCTTCACAACCTTTATCCAACCCTTcccttccg                                                                                                                                                           >  4:211514/1‑270 (MQ=14)
                               cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACACCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTACATACAg                                                                                                                                                           >  2:214237/1‑276 (MQ=255)
                                  aaTGGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                                                                                                                                         <  2:347881/275‑1 (MQ=255)
                                   taggcagaaTTTAGCGTGTTTTTGTGAGACGAGTAGGGCATTTATGCAGCATCCGACATCAACGCCTGATGCGACGCTGAACACGTCTTATTGAGCCGACGCCAGAAAGCGCAATATCCTGATTTAGCGGGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                                                                                                                                         <  3:183343/266‑1 (MQ=17)
                                    tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTca                                                                                                                                                                                      >  1:242573/1‑244 (MQ=255)
                                    tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAg                                                                                                                                                      >  2:452455/1‑276 (MQ=255)
                                       ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTCT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCAGGTATCAAACCCTTCCATACAGCTCAggc                                                                                                                                                    >  1:252044/1‑274 (MQ=255)
                                        cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                                                                  <  3:242573/244‑1 (MQ=255)
                                           aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCATACTCTTCACACCCGGTATCAAACCCTTCCATCCAGCTCAGGTTCAAc                                                                                                                                               >  2:225823/1‑276 (MQ=255)
                                             gtgtggTGGTTGTGTAGTGCGGATAAGGCGTTGATGCCGCAACCGACATCAACGCCTGATGGGACGCGTAACGGGGCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc                                                                                                                                               <  4:66390/269‑1 (MQ=18)
                                                 cGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATTAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGtt                                                                                                                                                                                                        >  4:187427/1‑213 (MQ=16)
                                                 cGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACTCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATCAGGCGTTT‑‑ACTC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTCTCAGGCCTATCTTACCCTTTGTTTAATTTTTCAAACTCTTCACCCCCGGTATCAAACCCTTCCACACAGCTCAgggttaaacctt                                                                                                                                           >  4:461006/1‑264 (MQ=17)
                                                 cGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGTTT‑‑ATGC‑C‑GA‑‑ATCCGCCATCAATGACTGCTGCGACGCTTGCCGCTCATTCTCAGCCCTATCTTAACCGTTGGTTAATTTTTGAAACTCTTCACACCTGGTACCAAACCCCTCCTTACACTTACCGTCCAACCAGTg                                                                                                                                          >  3:389209/1‑275 (MQ=14)
                                                 cGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTcac                                                                                                                                                                                     >  2:405887/1‑232 (MQ=255)
                                                    gATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAt                                                                                                                                                                                                     <  2:187427/213‑1 (MQ=25)
                                                    gATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                                                                  <  4:405887/232‑1 (MQ=255)
                                                    gATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                                                                                                                                         <  2:461006/257‑1 (MQ=255)
                                                        ttGTAGGTCGGATAGAGCGTTTATGCCGCAGCCGACATCATCGCCTGATGCGACGCTGAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCAGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCt                                                                                                                                  <  2:539025/276‑1 (MQ=18)
                                                                cGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCtt                                                                                                                           >  1:13478/1‑275 (MQ=255)
                                                                      aGGCGTTCATGCCCCATCCGACATCAACTCCTGATGCGAGGCGTAACTCGTCTGAACAGGCCTACGCCAGACAGCGCAATCGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCAACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCGTGttctt                                                                                                                     <  1:269741/275‑1 (MQ=21)
                                                                        gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTccca                                                                                                                                                                                    >  2:242942/1‑210 (MQ=255)
                                                                            ttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTATCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATTAGGCCTATCTTACACGTTGGATAATTTGTCAAACTCTTCACACCCGCTATCAAACCCTTCAATACAGCTCAGGTTCAACAATTTCACCGCCTTTGCCTTGTTctcctcta                                                                                                                >  1:196614/1‑272 (MQ=17)
                                                                             tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCACTGCCTGAGGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTACCCGTTGGTTAATTTTTCCAACCCTTCACACCCGCTATCAACCCCTTCCATCCACCACCGCTTCAACCAGTCACCCCCCTTTTCCttcttcttcaccat                                                                                                               >  4:558208/1‑269 (MQ=14)
                                                                             tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTTTTCTTCTCGATa                                                                                                              >  2:610480/1‑275 (MQ=255)
                                                                              tcgCCGCATCAGAAATCAACTCCTGATGCGACTCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAGTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATa                                                                                                              <  1:286953/272‑1 (MQ=255)
                                                                              aTGCCGCATCCGACATCAACGACTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACATCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATaaa                                                                                                            <  2:349825/276‑1 (MQ=255)
                                                                                   gCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCCATGCCTGCTGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTCATTTTTCAAACGTTTCACACCCGCTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGCTCAACTCCTTTTTCTTTTTCCTTTCGCTTAAACctt                                                                                                       >  4:302976/1‑276 (MQ=17)
                                                                                       ccaaCATCAAAGCGTGTTGGGTCGCTTAACGCGTTTTTTTAGGCGTACGCGAGACAGGGCAATAGACTGATTTAGCGTTATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑CC‑‑ATCCGACATTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACcttctt                                                                                                    <  3:196473/272‑1 (MQ=25)
                                                                                                 cGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCNTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACAAGTTCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCttttga                                                                                          >  4:335621/1‑273 (MQ=255)
                                                                                                       aTGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTATGTGGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTCGGTTAATTTTTCAAAGTCTTCACACCCGGTATCAAACCCTTCCATCCAGCCCAGGTTCAACCATTGCAGCGCCTTTTGCTTGTTCTTCCCGATAAAACCTTCTTCACCGTTTAAGAAcatc                                                                                   >  2:229787/1‑276 (MQ=21)
                                                                                                           gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTc                                                                               <  2:231306/276‑1 (MQ=255)
                                                                                                            aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTc                                                                               <  1:110513/275‑1 (MQ=255)
                                                                                                             cGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTCCCACACAGCCCAGGTTCAACCAGTGCTGCGCCTTTTGCTTGTTCTTCTTCATAAACCCTTCTTCAACGTTTCAGACACTCATTctc                                                                             >  4:160757/1‑274 (MQ=21)
                                                                                                                ttatccctgcTTATCGGGCGTACGCCAGGCAGGTCAATGTCCTGATTGAGCGTTATTTTGTAGGTCGGAGAAGGCGTGTCCT‑‑C‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCNTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGcc                                                                          <  4:333958/267‑1 (MQ=37)
                                                                                                                  aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGANATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGTTTCAACCAGTTCCGCGCCTTTTGCTTGTTCTTCTCGATACACCCTTCTTCACCGTTTAAAAACTTCATtccctcccc                                                                        >  4:326427/1‑275 (MQ=25)
                                                                                                                       gTCTTAACATGCCTACGCCAGACAGCGCAATGGCCTGATTTAGCGTGATTTTGTAGGGCGGATAAGGCGTTT‑‑ACTC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGGCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTAc                                                                    <  1:376897/275‑1 (MQ=255)
                                                                                                                       cgcTTATATGGCATGCGCCAGACAGCGCGATAGACTGATTTAGCGTGAGTTTGTAGGACGGTGAAGGCGTTG‑‑A‑‑C‑T‑GCTAAGCCGACATCAAGTGCGGAGTCGACGCTGGACGCGTCTTATCAGGCATATCTTAACCGTTGGTTAATTTTTCAAACGCTTCACACCCGGTATCAAACCCTTCCATACAGGTCAGGTTCAACCAGTGAAGCGCCTTTTGCGTGTTCTTCGCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTAc                                                                    <  3:126319/273‑1 (MQ=255)
                                                                                                                        tCTTATCAGGTCGACGCACGACAGCGCAATGGCCGGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTG‑‑ACGC‑C‑GA‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTc                                                                  <  2:89331/276‑1 (MQ=37)
                                                                                                                        tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACt                                                                   <  2:421054/275‑1 (MQ=255)
                                                                                                                         cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTCGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTCTCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATCCAGCTCAGGTTCAATCAGTGCAGCGCCTTTTGCTTGTTTTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCCCCCattccac                                                                  >  2:131617/1‑269 (MQ=25)
                                                                                                                            aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTGT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAACTCAGGTTCAACCAGTGCAGCGCACTTGGCCTGTTCTTATCTACAAAAACTTCTTACCCGATTAAGGACTTTTTTCCCTCCCACTACACCGaa                                                              >  4:612653/1‑276 (MQ=18)
                                                                                                                                      cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGtt                                                     <  2:211514/275‑1 (MQ=255)
                                                                                                                                      cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTGGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGTGAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGCATAACCGCtt                                                     >  3:363728/1‑275 (MQ=255)
                                                                                                                                           gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGa                                                <  3:13478/275‑1 (MQ=255)
                                                                                                                                           gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGa                                                <  1:371381/275‑1 (MQ=255)
                                                                                                                                           gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAATGCAGCGCCTTGTGCTTGTTCTTCTCGATAACACCTTCTTCACCGTTTAAGAACCTCCTTCCCCCCCAGTCCTCGGATTAACCGGTTc                                                    >  4:303735/1‑271 (MQ=37)
                                                                                                                                           gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGCTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCGCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGCTTCGGGaa                                               >  2:334503/1‑276 (MQ=255)
                                                                                                                                           gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAACGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCGCAGGTTCAACCAGTGCAGCGCCCTCTGCTTGATCTGCTCGATACAACCTTCTTTACCGTTTAAGAACATCATTCCCGCCAAGTACTCGGTATAACCGGTTCg                                                   >  1:404041/1‑272 (MQ=25)
                                                                                                                                             cggggcgagagacTGATTTAGCGTGATGTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCGGACATCAATGCCTGATTCGACGGTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGa                                                <  3:228671/261‑1 (MQ=38)
                                                                                                                                                cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCCAACCCTTCCATACAGCTCAGGTTCAACCAGTCCAGCGCCTTTTGCTTGTCCTTCTCGATAAAACCTTCTTCACCCTTTAAGAACACCATTCCAGCCCAGTACTCGGACTAACCTGTTCGGAACATTGc                                          >  4:54691/1‑276 (MQ=37)
                                                                                                                                                cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCTTTCCATACAGCTCAGGTTCAACCAGTGCAGCTCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTCTAAGAACATCATTCCCGCCCAGTACTCGGAATAACTGGTTCTGGAACTTg                                           >  1:363383/1‑275 (MQ=37)
                                                                                                                                                cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTg                                           >  1:127467/1‑275 (MQ=255)
                                                                                                                                                cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGc                                          >  2:436099/1‑276 (MQ=255)
                                                                                                                                                cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAAATCATTCCCGCCCAGTACCCGGAATAACCGGTTCGGTAAAtgt                                           >  3:390053/1‑273 (MQ=255)
                                                                                                                                                cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAAAACATCATTCCGGCCCAGTATTCGGCATAACCGGTTCGGGaatatgc                                          >  4:357838/1‑271 (MQ=255)
                                                                                                                                                cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTATTCACCGTTTAAGAACATCATTCCAGCCCAGTAACCGGAATAACCGGTTCGGCAAATTga                                          >  4:130295/1‑275 (MQ=255)
                                                                                                                                                  cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCCTCACCGTTTAAGAACATCATTCCCCCCCAGTACCAGGAATCACCGGTCCGGGAAATTTCa                                         >  3:396247/1‑275 (MQ=37)
                                                                                                                                                   aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTAGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTCTTCGGAATCACCGTTTCGGGAAATGTCaga                                       >  2:270771/1‑276 (MQ=37)
                                                                                                                                                           taattAGCTTGTTTTTGTAGGTGCGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGTGAAATTGCAGAGCTGCg                                 <  1:361833/271‑1 (MQ=39)
                                                                                                                                                           ggtttGGCGTGATTTTGTAGGGAGGAGAAGGCGTTTC‑AG‑C‑C‑GA‑‑ATCCGACAGCAAGGCCTTATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGtt                               <  4:473471/274‑1 (MQ=255)
                                                                                                                                                           gATGTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGtt                               <  2:548494/276‑1 (MQ=255)
                                                                                                                                                            aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGATCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTTTTCACCGTTTAAGAACATCATTccc                                                                             >  2:295775/1‑229 (MQ=255)
                                                                                                                                                                 gggggtttttGTAGGTCGGATAAGGCGTTT‑‑A‑‑CTCAGC‑‑AGCCGACATCAATGCCTGGTGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCNCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAAt                         <  4:334503/270‑1 (MQ=255)
                                                                                                                                                                 gCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCCTTCCCGCCCAGTACTCGGAATAATCGGTTCGGAAAATGACAGAACTGCGCTTGTAAt                         >  4:533349/1‑276 (MQ=38)
                                                                                                                                                                  cGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAACCTCTTCACACCCGGTATCAAACCCTTCAATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGCTAAAACCTTCTTCACCGTTTAAGAACATCTTTCCCCCCCAGTACTAGGAATAACCGGTCCGGGAAATTGCAGAGCTGCGTTTCaaa                          >  3:222198/1‑274 (MQ=38)
                                                                                                                                                                  cGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAACCCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCATTACTCGGACTACCCGGTTCGGGCAATTGCAGAGCTGCGTTTGAAAt                         >  3:312041/1‑275 (MQ=255)
                                                                                                                                                                  cGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACTGTTTAAGAACATCATTCCCGCCCATTACTCGGAATAACCGGTTCGGGAACTTGCAGAGCTGCGTTTgcacta                        >  4:158118/1‑271 (MQ=255)
                                                                                                                                                                      aTTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAACAGTGCAGCACCTTTCGCTTGTTTTTTTATATAAAACTTTTTTCACCGTTTAAGAACAACATTCTCGCCCCGTTCTCGATATAACCGGTTCGGGAAATTCCACAACTGTGTTTGAAATAcgtg                    >  4:356184/1‑273 (MQ=25)
                                                                                                                                                                       ttttGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GA‑‑AGACGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAgg                   <  2:533349/276‑1 (MQ=255)
                                                                                                                                                                                tCGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAg                                                                       >  3:85080/1‑215 (MQ=255)
                                                                                                                                                                                 cGGATAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGCTTGCCTTGt          >  1:377324/1‑275 (MQ=255)
                                                                                                                                                                                    aTAAGGCGTTT‑‑ACGC‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACt                                                                   <  1:85080/215‑1 (MQ=255)
                                                                                                                                                                                        ggCGTTT‑‑ACGC‑C‑GC‑‑ATCTGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCAtcgtcg  <  2:388700/276‑1 (MQ=255)
                                                                                                                                                                                                  ||                                                                                                                                                                                                                                                                              
TAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑C‑GC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCG  >  NC_000913/4296189‑4296646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑