Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,993,145 C→A R665S (CGT→AGT)  cyaA → adenylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,993,1450CA99.0% 275.5 / NA 96R665S (CGT→AGT) cyaAadenylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (44/50);  total (44/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

ATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCT  >  NC_000913/3992873‑3993415
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                              
gtttCCGTAAGCTTGATGTCTTGAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCGGCTGTACCGCAACGCGTGGAACGAAGTGCGTACGCTGAAATTCAACGGCAAGCAATCGATGATCGAAGCCCTAAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGt                                                                                                                                                                                                                                                                              <  3:101422/274‑1 (MQ=255)
 tttCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGt                                                                                                                                                                                                                                                                              <  3:271421/274‑1 (MQ=255)
 tttCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTc                                                                                                                                                                                                                                                                             >  2:179931/1‑275 (MQ=255)
          gCTGGGTGTCTTCACCTTAGGCACAGATAGAAATGGCGTGGTAGGGAGGGTTGACCTGCTTGACCGCAACGCGGGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCa                                                                                                                                                                                                                                                                    <  3:102054/275‑1 (MQ=255)
           cTGGATGTCTTAAGCTTTTGCGGTAATAAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCATACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGGAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCAcc                                                                                                                                                                                                                                                                  <  2:572899/276‑1 (MQ=255)
              gATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCATGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCAccccc                                                                                                                                                                                                                                                               >  2:466866/1‑274 (MQ=255)
                         cTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAAGCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATAAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTCCTGTTATAGCCAGCATCTGAGTGGCTGTATTCGTACTCGCGTGCAGCAATTTGTTTCTGAGCGTATTGAATTTATTCTTTACAGCACCCGCCAGGGAACCg                                                                                                                                                                                                                                                     >  3:285557/1‑275 (MQ=255)
                            tGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCg                                                                                                                                                                                                                                                 >  2:54973/1‑276 (MQ=255)
                              gCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAACGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGGCTTCTGTTATAGCCAGCAGTTGCGCGGCCTAATCCGTACTCGCGTGCAGTAACCGGTTTTTGAGTGTAGTGTATTGAGTCTTTCCAGACCCCGTCGGGAACACGGgtgt                                                                                                                                                                                                                                                >  3:144508/1‑275 (MQ=255)
                                   aaTCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGAGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGACGTTTCAAg                                                                                                                                                                                                                                          >  2:10270/1‑276 (MQ=255)
                                       aaaaTTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGgcg                                                                                                                                                                                                                                       <  3:19380/275‑1 (MQ=255)
                                        aaaTTGCCGGGTAGGTAGCTTTAACCCGCGTTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGgcg                                                                                                                                                                                                                                       <  3:241508/274‑1 (MQ=255)
                                              ccTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTCCAGCAACTGGTTTCTGAGTGTATTGCACTGAGTCTTTCCAGCACCCGCCTGGAAACCGGCCGTTTCAAGGCTCTGcgcg                                                                                                                                                                                                                                >  4:355724/1‑275 (MQ=255)
                                              ccTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCGTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTgtgc                                                                                                                                                                                                                                 >  1:206796/1‑274 (MQ=255)
                                                  gtgggtcggctttaCCTGCGATACCGCAACGCGTGGACAGAAGTGCGAACGCGGCACTTCAACGGCGAGGAATCGATGATCGACACCCTGAAAACTATTCTCTGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGttt                                                                                                                                                                                                                             <  3:373362/261‑1 (MQ=255)
                                                    agtgagggTTGAACTGGTGGAACGCAACTCGTGGAACGAAGTGCGTACGCGGCACTACAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATGCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTgg                                                                                                                                                                                                                         <  4:168158/269‑1 (MQ=255)
                                                      gtagCGTTGACCTCCTGTACCGCAATGCGTGGACCGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTc                                                                                                                                                                                                                       <  2:268011/276‑1 (MQ=255)
                                                        agCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCCACTGGTTTCTCAGTGTATTGACTTGAGTCTTTCCAGCACCCTCCAGCACACCGGGCGTTTCCATGCGCTTCTCGTTTCTGGTcca                                                                                                                                                                                                                     >  4:223373/1‑274 (MQ=255)
                                                        agCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCa                                                                                                                                                                                                                      <  3:48508/275‑1 (MQ=255)
                                                        agCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGAACGCCGCACCGCCAGACAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTAGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCAAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCaa                                                                                                                                                                                                                     >  2:434433/1‑276 (MQ=255)
                                                            ttGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTCCGTACGGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACGGGGCTTTTCACGGGGCTCGTCTTTTCTGGTAAAAc                                                                                                                                                                                                                   >  3:201166/1‑274 (MQ=255)
                                                                    ctgTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCTGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGACTTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGGGTTTCAAGGCGCCGCGCGTTTCTGGCCCACCCTGGTGgtt                                                                                                                                                                                                          >  4:12870/1‑275 (MQ=255)
                                                                    ctgTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCTAGGCGCTGAGCTTTTCTGGTCAACCCTGGGGgtt                                                                                                                                                                                                          >  4:20167/1‑275 (MQ=255)
                                                                           gCAACTCGTGGAACGAAGTGAGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCg                                                                                                                                                                                                  <  2:451277/276‑1 (MQ=255)
                                                                           acAACTCGCGGGACGAAGTGCGTACGCTGCCCTTCAACGGGGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCATGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCg                                                                                                                                                                                                  <  2:47211/275‑1 (MQ=255)
                                                                            cAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGGACTCGCGTGCAGCAACTGGTTTCTGCGTGTATTGAATTGAGTCTTTCCATCACCCGCCAGGAAACCGGGCGTTTCAAGGAGCTGCGCGTTTCTGGGCAAACCTGGGGGTTGTGGTTCGa                                                                                                                                                                                                 >  4:451277/1‑276 (MQ=255)
                                                                             aaCTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGaa                                                                                                                                                                                                <  2:605122/276‑1 (MQ=255)
                                                                              acgcGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGa                                                                                                                                                                                                 <  1:315023/271‑1 (MQ=255)
                                                                              aCTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAAc                                                                                                                                                                                               >  2:330958/1‑276 (MQ=255)
                                                                               cTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTGCTTCGAACg                                                                                                                                                                                              >  2:604319/1‑276 (MQ=255)
                                                                                 cGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGCAACCGGGCGTTTCAAGGCGCTGCCCGTTTCTGGTCAAAACTGGGGGTGGTGCTTCGAAcccc                                                                                                                                                                                            >  4:237336/1‑276 (MQ=255)
                                                                                 cGTGGAACGAAGTGCGTACGCTGCACTTAAACTGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGc                                                                                                                                                                                             <  3:243621/275‑1 (MQ=255)
                                                                                      aaCGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATTGATGATCGAAGCCCTGAAAACTGTTCTCGGCAAAATGCATCAGGACGCCGCAGCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCGTCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGaa                                                                                                                                                                                        <  1:329695/275‑1 (MQ=255)
                                                                                           aatGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCATGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTAt                                                                                                                                                                                   <  1:144508/273‑1 (MQ=255)
                                                                                                    gCTGCACTTCAACTGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACCCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGaa                                                                                                                                                                         <  2:107866/276‑1 (MQ=255)
                                                                                                      tGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCCGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGCATGTTTCGGTACAGaa                                                                                                                                                                         >  1:292981/1‑274 (MQ=255)
                                                                                                       gCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCCAACCTGGGGGTTGTTCTTCGTACGCCTGAATGTATCGGTAACGaaa                                                                                                                                                                        >  1:262050/1‑274 (MQ=255)
                                                                                                                cGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCCCCCGCCAGGACACCGGGTGTTTCAAGGCGCTGCGCGTTTCTGGTCCACCCTGGGGGTTGCTGTTCGAACCCCGGCATGTTTGGGTAGAGGAACCTGTAAACGc                                                                                                                                                             >  4:229360/1‑276 (MQ=255)
                                                                                                                cGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTGGTTTTTGGAACTCCTGGATGGTTCGGTTCAGAACTGGGAAAAGc                                                                                                                                                             >  3:217417/1‑275 (MQ=255)
                                                                                                                    gAGCAATCGATGATAGACACCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGGGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCa                                                                                                                                                           <  1:285557/274‑1 (MQ=255)
                                                                                                                     aGCCATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATc                                                                                                                                                         <  1:217417/275‑1 (MQ=255)
                                                                                                                     aGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTGCCAGCACCCGCCAGGAAACCGGGCGTTTAAAGGTGATGCGCGTTTCTGGTCAATCCTGGGGGTTGTTCTTCGAAGGCCTGAATGTCTCGGTACAGACACTGGCAAACGcc                                                                                                                                                            >  1:330692/1‑272 (MQ=255)
                                                                                                                                  cGAAGCCCTGAAAACTAGTCTCGGCAAAATGCATCAGGACGCCGCTCCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGgcg                                                                                                                                            <  2:355724/275‑1 (MQ=255)
                                                                                                                                       cactaaaaaCGAGTCTCGGCAAAATGCAACATGGCGCCGCACCGCCAGGTAGCGTGGAAGTCTTCTGTTATAGCCATCATCGGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTTAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGAtt                                                                                                                                       <  1:90679/270‑1 (MQ=255)
                                                                                                                                          tGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCaaa                                                                                                                                                                                                                    >  2:59075/1‑195 (MQ=255)
                                                                                                                                             aaaCTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCt                                                                                                                                                                                                                 <  4:59075/195‑1 (MQ=255)
                                                                                                                                              aaCGATTCTCGGGAAAATGCATCAGGACGCCGGACCGCAAGATAGAGTGGAAGGCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATa                                                                                                                                <  2:12870/275‑1 (MQ=255)
                                                                                                                                                   ttCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACTCCTGAATGTATCGGTACAGAAACTGGAAAAACCCATCGAGCTTTATGGCTCGATTTTGCGTAACaaa                                                                                                                           >  3:265843/1‑275 (MQ=255)
                                                                                                                                                   ttCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACaaa                                                                                                                           >  2:584239/1‑275 (MQ=255)
                                                                                                                                                       cGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGc                                                                                                                       <  1:301324/275‑1 (MQ=255)
                                                                                                                                                       cGGCAAAATGCATCACGACGCCGCACCGCCAGATAGCGTTGAAGTCTTCTGGTATAGCCAGCCTCTGCGCGGCTTAAGTCGTACGCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGATACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCa                                                                                                                      <  2:138518/276‑1 (MQ=255)
                                                                                                                                                        ggCAAAAGTCAGCATGACACCTCACCGCCAGAAAGCGTGGAAGTCTTCTGTTATAGCTAGCATGTGAGCGGCTTAATTCGTACTCGCGGGCAGCAACTGGTTTCTGAGTGTCTTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCa                                                                                                                      <  3:139908/275‑1 (MQ=255)
                                                                                                                                                        ggCAAAAGGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCACCTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCa                                                                                                                      <  1:318012/275‑1 (MQ=255)
                                                                                                                                                          cAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACgg                                                                                                                   <  2:20167/276‑1 (MQ=255)
                                                                                                                                                                    cAGGACCGCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTg                                                                                                         <  2:473696/276‑1 (MQ=255)
                                                                                                                                                                            cgcACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTTGACTAACAAACTGCCCGCGCTGGCAGTGCATGTTGa                                                                                                 >  4:483217/1‑276 (MQ=255)
                                                                                                                                                                                tcGCCAGCGAGCGTGGGAAGATTCGTTTATAGCCAGCATCTGGGCGGCTTAATTCGTACTCGCGTGCAGCAACTTGTTTCGTAGGTTATTGAATTGAGTCTTTCCAGCAGCCGCCAGGAGACCGGGCGTTTCAAGGCGCTGCGAGTTTCTGGTCAAACCTGGGGGTTGTTCGTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAAc                                                                                              <  3:330692/274‑1 (MQ=255)
                                                                                                                                                                                 cGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGt                                                                                                                                                                                                                                                                              <  2:119549/98‑1 (MQ=255)
                                                                                                                                                                                   ccAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCTCGGCCTGTCAGTGCAGGTTGAAACCa                                                                                            >  1:65688/1‑274 (MQ=255)
                                                                                                                                                                                                     ttctGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAAAGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAAGCAATCACGTCAAATTACcggc                                                                         >  1:38383/1‑275 (MQ=255)
                                                                                                                                                                                                              ttccAGCATTTGCGCGGTTTAATTCGTACCCGCGTGATGTAACTGGTTTCTGAGTGTATTGAATTTAGTCTGTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCGGGGGGTTGTTCTTCGAACGCCTGAAGGTATCGGTAAAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGtggtgg                                                                 <  3:194815/272‑1 (MQ=255)
                                                                                                                                                                                                              agcacgCATCTGCGCGGCTTAATTCGTACTCTCGTGCAGCAACTGTGTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGtggtgg                                                                 <  3:262050/269‑1 (MQ=255)
                                                                                                                                                                                                               gCCACCAGCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTTGTTTCTGAGTGTATTGCATTGAGTCTTTCCAGCACCCGCCACGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTGCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGa                                                                <  1:206069/274‑1 (MQ=255)
                                                                                                                                                                                                                  tgcctgtggacgGCTTAATTTGGATGGGCGTGCCGCAACTCGTTTTGGGGTTGATTGTAGTGCGTCTTGCCGGGACGCGCCCGGAAACCGGTCGTTTCAAGGCGCTGCGCGTTGCGGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGc                                                            <  4:466866/265‑1 (MQ=255)
                                                                                                                                                                                                                  aGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACTTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCGGTGCAGGTTGAAACCACTCACGTCAAATTACCGGCGGGGGTGGAGGGCt                                                           >  2:278470/1‑276 (MQ=255)
                                                                                                                                                                                                                  aGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAAAGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTCCACGGCCTGTCAGGGCAGGTGGAAACCACGCACGACAAATTACAGGACGTGGTGGACgggt                                                           >  4:46103/1‑274 (MQ=255)
                                                                                                                                                                                                                  aGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGCTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGCTTTCGCATAACAAACTGCACGACCTGTCAGTGCAGGTCGAAACCAATCACGTCAAATTACCGGCGGGGGTGGACGGCt                                                           >  2:509013/1‑276 (MQ=255)
                                                                                                                                                                                                                    cATCTGCGCTGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACTCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGAGCGATTTCGCATAACAAACTGCACGGCCTGTCCGTTCAGGTTGAAACCAATCACGTCAAATTACCGGCGgtggtgtaggggtt                                                          >  3:160182/1‑267 (MQ=255)
                                                                                                                                                                                                                    cATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGttct                                                                                                                                                                                                     >  1:6650/1‑136 (MQ=255)
                                                                                                                                                                                                                    cATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTc                                                                                                                                     >  4:314393/1‑200 (MQ=255)
                                                                                                                                                                                                                    cATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCCCGGCCTGTCAGTGCAGGTTGAACCCCATAACGTCAAATTACCGGCGGTTGTGGACGGCttt                                                         >  4:386330/1‑276 (MQ=255)
                                                                                                                                                                                                                    cATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGGCAGTGCAGGTTGACACCAATCACGCCAAATTAACGGCGGTGGTGGACGGCttt                                                         >  2:382759/1‑276 (MQ=255)
                                                                                                                                                                                                                    cATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGCGCATTGAATTGAGTCTTTCCAGCACCCGCCAGGACACCGGGCGTTTCAAGGCGCTGCGCGTTTCTCGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGACACTGGAAAACGCCATCGAGTTTTATGTCGCGCTTTTGCCTCACAAACTGCCCGGCCTGTAAGTGCAGGTTGCAACCCATCCCGTCAAATTAACGGCGGTAGCGGACGGCt                                                           >  3:230876/1‑274 (MQ=255)
                                                                                                                                                                                                                       cTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCa                                                                                                                                  <  2:314393/200‑1 (MQ=255)
                                                                                                                                                                                                                       cTGCGCGGCTTAATTCGTACGCGCGGGCAGCGACTGGGTTCTGAGTGTATTGAACTGAGTCTTGCCAGCACCCGCGAGGAAACCGGGCGGTTCAGGGCGCTGCGCGTTTCTGGTTAAACCTGGGTGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAAGCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGc                                                       <  2:613832/275‑1 (MQ=255)
                                                                                                                                                                                                                        tGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGa                                                                                                                                                                                                 <  3:6650/136‑1 (MQ=255)
                                                                                                                                                                                                                        tGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTCTCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGGGCAGGTTGAACCCAATCACGTCAAATTACCGGCGGTGGTTGACGGCTTTGc                                                       >  1:194815/1‑274 (MQ=255)
                                                                                                                                                                                                                          cgcgGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGACCGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGAACGGCCGGTCGGTGCAGGTTGAAACCAATCCAGTCAAATTACCGGCGGAGGTGGACGGCGTTGCAAGc                                                   >  2:325403/1‑276 (MQ=255)
                                                                                                                                                                                                                             gtgttacgtCGTACTCGCGTGCAGCAACTGGATTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGa                                                 <  3:118947/267‑1 (MQ=255)
                                                                                                                                                                                                                             ggCTTAAGTCGTCCGCACGTGCAGCAACTTGGTTCTGAGTGTATTGAATTGAATCTCTCCAGCGCCCGCCAGGAAACCGGGCCTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTAGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGa                                                 <  4:10270/275‑1 (MQ=255)
                                                                                                                                                                                                                              gtgtAATACGTACTCGCGCGCGGCAACTGGTTTCTGAGTGTATTGAGTTGAGTGTTAACCACACCCGCCATGAAACCTGGCGTTTCAAGGCGCTGCGAGTTTCTGGTCAAACCGGGGGGTTGTTCTTCGAACGCATGAATGTATCGGGACAGAAACTGGAAAACTCCATCGAGTTTTATGGAGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGa                                                 <  3:206796/271‑1 (MQ=255)
                                                                                                                                                                                                                               cTTAATTCGTTCTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGa                                                 <  1:201166/273‑1 (MQ=255)
                                                                                                                                                                                                                                   attccgtctagcgTGCAGGAACGTGTTTCTGTGTTTATTGAATTGGGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTTTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGa                                            <  1:288338/264‑1 (MQ=255)
                                                                                                                                                                                                                                   agtCGTACTAGCGTGCAGCAACTGGTTTCTGAGGGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGa                                            <  1:9582/272‑1 (MQ=255)
                                                                                                                                                                                                                                      ctgcttctgtgtcagcaACTGTGTTCTGAGTGTATTTAACTGAGTCTTTACAGGCCCCGCAAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGGCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGGACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGatcatc                                       <  4:434433/264‑1 (MQ=255)
                                                                                                                                                                                                                                         aCTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTTCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGAGGGCTTTGCCCGCGAAGGGACAATCCAg                                    >  4:447823/1‑276 (MQ=255)
                                                                                                                                                                                                                                         aCTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGCTCATCCa                                     >  1:366708/1‑275 (MQ=255)
                                                                                                                                                                                                                                              cgTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATCACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCt                                >  2:355545/1‑275 (MQ=255)
                                                                                                                                                                                                                                                       acgggTTTCTGGGTTGATTGAATTGAGTCTTTCCAGCACCGGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCGGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCgaaga                      <  2:372292/273‑1 (MQ=255)
                                                                                                                                                                                                                                                                   gtgtATTGGATTGAGTCTTTCCAGCACCCGCCAAGAGACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGAAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGAc            <  2:412972/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                     gtATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGttcttc                                                                                                                                                                                                   >  4:329177/1‑89 (MQ=255)
                                                                                                                                                                                                                                                                      tATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCCGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACgag         >  2:388464/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                       agtGAATTGAGTCGTTCCAGCACCCGCCAGGACACCGGACGTTTCAAGGGGCGGCGCGTTTCGGGGTAAACCTGGGGGGTGGTCTTCGAACGCTTGAATGTATCGGGACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGaa       <  4:23301/273‑1 (MQ=255)
                                                                                                                                                                                                                                                                        ttGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGaa                                                                                                                                                                                                <  2:329177/89‑1 (MQ=255)
                                                                                                                                                                                                                                                                           aaTTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCt  <  2:483217/276‑1 (MQ=255)
                                                                                                                                                                                                                                                                            aTTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCt  <  1:160182/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                              
ATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCT  >  NC_000913/3992873‑3993415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: