New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 4254356251 (1.220)4 (0.020) 4/316 NT 1.7% coding (2111/2424 nt) plsB glycerol‑3‑phosphate O‑acyltransferase
?NC_000913 = 4254384 231 (1.150)coding (2083/2424 nt) plsB glycerol‑3‑phosphate O‑acyltransferase
Rejected: Frequency below/above cutoff threshold.

GCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCATAACGTTGCAGCGTTTCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4254206‑4254356
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcgcACGCTACAGCTGCTGGCCGCAGGCGCGCGCGAAACGCTGCAACGTTATGCCATCACCTTCTGGTTGTTGAGTGCCAACCCGTCGATCAACCGCGGTACGCTGGAGAAAGAGAGCCGCACCGTCGCGCAACGTCTCTCCGTGCTGC  <  NC_000913/4254384‑4254240
                                                                                                                                                                                                                                                                                                        
GCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCATAACGTTGCAGCGTTTC                                                                                                                                                     <  6:176242/149‑1
GCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCATAACGTTGCAGCGTTT                                                                                                                                                      <  8:758268/148‑1
GCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCATAACGTTGCAGCGTTT                                                                                                                                                      <  8:958061/148‑1
 CCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCATAACGTTGCAGCGTTTCG                                                                                                                                                    >  5:209349/1‑149
  CTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCATAACGTTGCAGCGTTTCGC                                                                                                                                                   <  6:497164/149‑1
                                     GCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCATAACGTTGCAGCGTTTCGCACGCTACAGCTGCTGGCCGCAGGCGCGCGCGAAAC                                                                                                                <  6:497552/149‑1
                                         GGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCATAACGTTGCAGCGTTTCGCACGCTACAGCTGCTGGCCGCAGGCGCGCGCGAAACGCTG                                                                                                            <  5:863094/149‑1
                                                                  CTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCATAACGTTGCAGCGTTTCGCACGCTACAGCTGCTGGCCGCAGGCGCGC                                                                                                                       >  5:632870/1‑113
                                                                  CTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCATAACGTTGCAGCGTTTCGCACGCTACAGCTGCTGGCCGCAGGCGCGC                                                                                                                       <  6:632870/113‑1
                                                                                                                                                   TCGCACGCTACAGCTGCTGGCCGCAGGCGCGCGCGAAACGCTGCAACGTTATGCCATCACCTTCTGGTTGTTGAGTGCCAACCCGTCGATCAACCGCGGTACGCTGGAGAAAGAGAGCCGCACCGTCGCGCAACGTCTCTCCGTGCTGC  >  6:872692/1‑149
                                                                                                                                                                                                                                                                                                        
GCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCATAACGTTGCAGCGTTTCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4254206‑4254356
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcgcACGCTACAGCTGCTGGCCGCAGGCGCGCGCGAAACGCTGCAACGTTATGCCATCACCTTCTGGTTGTTGAGTGCCAACCCGTCGATCAACCGCGGTACGCTGGAGAAAGAGAGCCGCACCGTCGCGCAACGTCTCTCCGTGCTGC  <  NC_000913/4254384‑4254240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.